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Overview of protocols of Golden Gate cloning for assembly of multiple DNA fragments.

DNA Assembly and Cloning

DNA assembly and cloning are critical techniques in synthetic biology that enable researchers to create and modify DNA sequences for various applications, including gene editing, protein engineering, and synthetic biology. Traditional cloning methods, such as restriction enzyme digestion and ligation, can be time-consuming and often result in unwanted mutations or errors. However, recent advancements in DNA assembly and cloning techniques have enabled researchers to construct complex DNA sequences with greater speed, accuracy, and efficiency.

Potential uses of synthetic genetic circuits.

Genetic Circuits Design

Genetic circuits are complex systems of interacting genes that can be designed to perform specific functions, such as producing valuable chemicals or sensing environmental signals. The field of synthetic biology has enabled the creation of increasingly sophisticated genetic circuits, which has great potential for various industries. CD Biosynsis provides a wide range of services for genetic circuits design in this field.

The abstract formation of an orthogonal DNA replication system by Plasmid/DNA polymerase pair.

Orthogonal Genetic Systems in Strains

In recent years, the field of synthetic biology has made significant advancements in developing new genetic systems that allow for precise and efficient control of gene expression and editing. One promising approach is the use of orthogonal genetic systems, which enable the simultaneous regulation and editing of multiple genes with high specificity and efficiency. CD Biosynsis is leading the way in providing cutting-edge services in this exciting orthogonal genetic systems field.

Biocontainment strategies and biosafety guidelines.

Biocontainment Strategies in Strains

In the field of synthetic biology, genetically modified organisms (GMOs) have been engineered to produce a variety of products at an industrial scale. However, concerns have been raised surrounding the ecological risks associated with the release of GMOs outside of controlled environments. Biocontainment systems are needed to neutralize these GMOs and prevent them from causing harm to the environment. In addition, alternative selection markers are needed to replace costly and risky antibiotics.

Key advantages of cellular and cell-free bioproduction.

Cell-Free Systems

Cell-free systems are gaining popularity as a platform for protein synthesis due to their rapid and efficient nature. These systems are free from cell walls and membranes and can be used to synthesize proteins directly from DNA templates. They offer several advantages over traditional methods of protein expression, including increased speed, simplicity, and cost-effectiveness. Synthetic biology has played a significant role in advancing cell-free systems by providing new methods for DNA synthesis and assembly, as well as new enzymes and substrates for protein synthesis.

A whole-cell biocatalytic strategy was developed to acylate esculin, and the introduction of medium- and long-length fatty acids chains in acylation endowed esculin with non-selective cytotoxicity.

Whole-Cell Biosynthesis

Whole-cell biosynthesis is a rapidly growing field in synthetic biology that involves the use of living cells as a platform for the production of valuable compounds. This approach offers a number of advantages over traditional chemical synthesis, including improved efficiency, reduced waste, and the ability to use renewable resources. CD Biosynsis is a leading company in the field of whole-cell biosynthesis, providing a range of services for the production of a wide variety of compounds using genetically modified microorganisms.

The non-recombinant approach to display antigens and enzymes on the surface of bacterial spores.

Spore Engineering

Spore engineering is a cutting-edge technology that has revolutionized the field of synthetic biology. It involves the genetic modification of bacterial spores, which are highly resistant structures that can survive harsh environmental conditions. Spore engineering has various applications in the fields of bioremediation, bioproduction, drug delivery, and biosensing. CD Biosynsis is a leading company in spore engineering, providing innovative solutions to meet the needs of our clients.

Elimination of the apramycin resistance gene in the host strain genome.

Antibiotic Resistance Marker Removal

The use of antibiotic resistance genes as selection markers in synthetic biology strains has been a common practice for many years. However, the increasing concern over the spread of antibiotic resistance has prompted scientists to develop alternative selection systems. The use of antibiotic-free selection systems is not only a safer option but also more cost-effective in the long run.

Schematic diagram of the biodegradation of toxic recalcitrant compounds.

Biodegradation Pathway Engineering

Synthetic biology has emerged as a promising approach for engineering microorganisms to perform complex metabolic tasks, including biodegradation. Biodegradation pathway engineering involves the modification of microbial metabolism to degrade specific contaminants or pollutants. At CD Biosynsis, we offer a range of services for biodegradation pathway engineering, utilizing the latest synthetic biology tools and techniques to design and optimize microbial consortia for biodegradation applications.

Major aspects of the de novo protein design.

De Novo Protein Design

De novo protein design is revolutionizing synthetic biology by enabling the creation of tailor-made proteins with unprecedented functions. Our services empower industries and researchers to harness the potential of these engineered biomolecules for diverse applications, from therapeutics to materials science.

Fig 1. Sustainable production of chemicals by combining industrial process optimization and genetic engineering approaches.

Chemical production Metabolic pathways Engineering

CD Biosynsis is committed to providing high-quality services to our clients. Our specialized synthetic biology platform specializes in providing tools, libraries of gene parts for metabolic pathways, and bioengineering for chemical production, as well as designing and optimizing metabolic pathways for maximum efficiency.

Fig 1. Direct combinatorial pathway optimization.

Biosynthesis Pathway design and optimization services

CD Biosynsis has expertise in pathway engineering, computational tools, and collaborative approaches that are well suited to meet the diverse needs of our clients in synthetic biotechnology.

Service process flow for site-directed mutagenesis at CD Biosynsis.

Site-Directed Mutagenesis

Site-directed mutagenesis is a very useful in vitro technique to create specific, targeted mutations in a known DNA sequence. It can efficiently change the characterization of target proteins. Site-directed mutagenesis can be used as a precision tool to enable synthetic biology. It plays an essential role in a variety of synthetic biology research, such as the study of gene regulatory elements, protein structure and functions, enzyme active sites and novel proteins.

Figure 1. Comparison of various commercial recombinant protein expression systems. (Raskin I, et al., 2002)

Cell-Based Protein Expression

CD Biosynsis has a team of experienced and highly skilled scientists to provide synthetic biology researchers around the world with high-quality cell-based protein expression services to facilitate their cutting-edge research.

Figure 1. Schematic diagram of the backbone building process at CD Biosynsis.

Protein Backbone Building

CD Biosynsis provides professional backbone building services for synthetic biology researchers around the world to facilitate their cutting-edge research. Our aim is to obtain protein backbone configurations that satisfy a set of predefined requirements by the customers.

CRISPR‒Cas9-based genome-editing approaches.

Genetic Engineering

Genetic engineering has revolutionized various industries, including healthcare, agriculture, and biomanufacturing. The field of synthetic biology, which involves the design and construction of new biological systems or redesigning existing ones, has further expanded the possibilities of genetic engineering. Genetic engineering has been a rapidly evolving field over the past few decades, with the development of new technologies and techniques that have made it possible to manipulate genes with unprecedented precision and efficiency. The potential of genetic engineering to revolutionize various industries has been well-established, but it also raises ethical and regulatory challenges that need to be addressed.

Diagram of modular plasmid design.

Plasmid Design and Transformation

Plasmids are small, self-replicating circular DNA molecules that play a crucial role in synthetic biology. They are used to introduce genes of interest into host cells, allowing for the manipulation and engineering of biological systems.

DNA Assembly and Cloning Services

DNA Assembly and Cloning Services

Through DNA assembly and cloning services, CD Biosynsis aims to provide researchers and innovators with streamlined access to complex genetic constructs, which will alleviate the technological challenges associated with DNA manipulation and enable researchers to focus on their core objectives, facilitating their breakthroughs in different fields.

Figure 1. Large-scale reengineering of organisms. (Standage-Beier K & Wang X, 2017)

Genome Reprogramming Service

CD Biosynsis provides custom genome reprogramming services for synthetic biology researchers around the world to facilitate their cutting-edge research. Our strong expertise coupled with advanced techniques can help bring diverse synthetic applications and creative ideas to fruition.

Novel CRISPR System

Novel CRISPR Gene Editing Service

In a world where scientific breakthroughs hinge on innovative tools, our novel CRISPR system stands at the forefront of genetic engineering innovation. With its precision, versatility, and diverse applications, it empowers researchers and industry leaders to push the boundaries of what's possible in the realm of synthetic biology.

Fig 1. Unbiased libraries in protein directed evolution.

Protein library design

CD Biosynsis's synthetic biology platform is dedicated to providing high-quality protein library design services to help biologists around the world design protein variant collections to support innovation in a variety of fields.

(CF Peddle, et al.,2017)

sgRNA Design Service

SgRNA (short guide RNA) design is the process of designing RNA molecules for the CRISPR-Cas9 genome editing tool. The CRISPR-Cas9 system is composed of Cas9 protein and guiding RNA (sgRNA), which can accurately cleave specific DNA sequences, thereby achieving genome editing and repair.

Strain Engineering for Protein and Peptide

CRISPR/Cas9 genome editing system(Y Cui, et al.,2018)

CRISPR-Cas9 sgRNA synthesis

CRISPR-Cas9 sgRNA synthesis refers to the use of molecular biology techniques to synthesize sgRNA (single guide RNA) molecules in the CRISPR-Cas9 system. CRISPR-Cas9 is a technology used for gene editing that can accurately modify DNA sequences. SgRNA is a key component of the CRISPR-Cas9 system, which can guide Cas9 enzymes to locate target DNA sequences and perform cleavage or repair. Therefore, synthesizing sgRNA is a necessary step for conducting CRISPR-Cas9 gene editing experiments. The synthesis of sgRNA is usually achieved through in vitro synthesis methods, using chemical synthesis or in vitro transcription.

Strain Engineering for Industrial Enzyme

 Engineering protein, metabolism and artificial cell in the open cell-free system.

Biosynthesis Cell free system

CD Biosynsis harnesses the transformative power of the Cell-Free System to catalyze innovation across the biotech landscape. With an unwavering focus on precision, quality, and versatility, we empower researchers and biotech entities to explore new horizons and unlock the full potential of molecular biology. Join us on the journey to redefine the future of biotechnology through our pioneering Cell-Free System Services.

Biosynthetic gene cluster and proposed biosynthetic pathway of 1.

Biosynthesis Gene Cluster

CD Biosynsis specializes in Biosynthesis of gene cluster services, offering cutting-edge solutions for synthetic biology applications. Our comprehensive services encompass gene cluster analysis, design, and optimization to drive innovation in biotechnology.

Schematic representation of cell-free methodology modification for the production of diverse proteins.

Protein Biosynthesis

CD Biosynsis specializes in cutting-edge protein biosynthesis services. Leveraging synthetic biology techniques, we deliver precise and efficient protein synthesis solutions tailored to your unique research and industrial needs.

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