Mutagenesis is a powerful tool that enables researchers to generate genetic diversity in microorganisms and other organisms. It involves the induction of mutations in the genome of an organism, which can result in the generation of novel phenotypes that may have beneficial properties for various applications, including industrial biotechnology. Synthetic biology has been instrumental in the development of novel methods for mutagenesis that enable the generation of large libraries of mutants with diverse phenotypes.
CD Biosynsis offers services for mutagenesis in strains from a synthetic biology perspective to help researchers in their work.
Figure 1: DNA mutation mechanisms by physical and chemical mutagenesis. (Trovão, M., Schüler, L.M., et al. 2022)
Our mutagenesis services are designed to help researchers generate genetic diversity in microorganisms and other organisms, which can result in the generation of novel phenotypes with beneficial properties for various applications, including industrial biotechnology:
Chemical mutagenesis involves the use of chemical agents that induce random mutations in the genome of an organism. At CD Biosynsis, we use a range of chemical mutagens, including
ethyl methanesulfonate (EMS),
diethyl sulfate (DES),
to induce random mutations in the genome of microorganisms. Chemical mutagenesis is a widely used method for generating mutant libraries, and it has been used successfully to generate mutants with desirable phenotypes for various applications, including the production of biofuels and bioplastics.
Site-directed mutagenesis involves the targeted introduction of specific mutations at precise locations in the genome. At CD Biosynsis, we use a range of site-directed mutagenesis techniques, including
to introduce specific mutations in the genome of microorganisms. Site-directed mutagenesis is a powerful tool for engineering specific traits in microorganisms, and it has been used successfully to engineer microorganisms for various applications, including the production of high-value chemicals and pharmaceuticals.
Random mutagenesis involves the introduction of random mutations in the genome of an organism using physical or chemical methods. At CD Biosynsis, we use a range of random mutagenesis techniques, including high-throughput sequencing, error-prone PCR, and transposon mutagenesis, to introduce random mutations in the genome of microorganisms.
We use NGS to sequence the genomes of mutant libraries and identify mutants with the desired phenotypes. NGS enables us to identify mutants with high precision and sensitivity, which can save our clients time and resources in downstream screening and characterization.
We work closely with our clients to develop mutagenesis strategies that are optimized for their research goals, and we provide comprehensive support throughout the mutagenesis process, including library construction, screening, and characterization.
At CD Biosynsis, we offer a range of services for mutagenesis in strains, including chemical mutagenesis, site-directed mutagenesis, and random mutagenesis. Our services are tailored to the specific needs of our clients, and we work closely with them to develop mutagenesis strategies that are optimized for their research goals. We are able to generate high-quality mutant libraries that are enriched for mutants with the desired phenotypes. Contact us today to learn more about our mutagenesis in strains and how we can help optimize your microbial strains for maximum performance.
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.