Introduction
Mutagenesis is a powerful tool that enables researchers to generate genetic diversity in microorganisms and other organisms. It involves the induction of mutations in the genome of an organism, which can result in the generation of novel phenotypes that may have beneficial properties for various applications, including industrial biotechnology. Synthetic biology has been instrumental in the development of novel methods for mutagenesis that enable the generation of large libraries of mutants with diverse phenotypes.
CD Biosynsis offers services for mutagenesis in strains from a synthetic biology perspective to help researchers in their work.
Figure 1: DNA mutation mechanisms by physical and chemical mutagenesis. (Trovão, M., Schüler, L.M., et al. 2022)
Our Services for Mutagenesis
Our mutagenesis services are designed to help researchers generate genetic diversity in microorganisms and other organisms, which can result in the generation of novel phenotypes with beneficial properties for various applications, including industrial biotechnology:
Chemical Mutagenesis
Chemical mutagenesis involves the use of chemical agents that induce random mutations in the genome of an organism. At CD Biosynsis, we use a range of chemical mutagens, including
ethyl methanesulfonate (EMS),
N-methyl-N'-nitro-N-nitrosoguanidine (MNNG),
diethyl sulfate (DES),
to induce random mutations in the genome of microorganisms. Chemical mutagenesis is a widely used method for generating mutant libraries, and it has been used successfully to generate mutants with desirable phenotypes for various applications, including the production of biofuels and bioplastics.
Site-Directed Mutagenesis
Site-directed mutagenesis involves the targeted introduction of specific mutations at precise locations in the genome. At CD Biosynsis, we use a range of site-directed mutagenesis techniques, including
PCR-based mutagenesis,
CRISPR-Cas9,
homologous recombination,
to introduce specific mutations in the genome of microorganisms. Site-directed mutagenesis is a powerful tool for engineering specific traits in microorganisms, and it has been used successfully to engineer microorganisms for various applications, including the production of high-value chemicals and pharmaceuticals.
Random Mutagenesis
Random mutagenesis involves the introduction of random mutations in the genome of an organism using physical or chemical methods. At CD Biosynsis, we use a range of random mutagenesis techniques, including high-throughput sequencing, error-prone PCR, and transposon mutagenesis, to introduce random mutations in the genome of microorganisms.
Next-Generation Sequencing
We use NGS to sequence the genomes of mutant libraries and identify mutants with the desired phenotypes. NGS enables us to identify mutants with high precision and sensitivity, which can save our clients time and resources in downstream screening and characterization.
Comprehensive Support
We work closely with our clients to develop mutagenesis strategies that are optimized for their research goals, and we provide comprehensive support throughout the mutagenesis process, including library construction, screening, and characterization.
Our Advantages
- Expertise
Our team of experienced scientists has extensive expertise in mutagenesis and synthetic biology, and we have a track record of success in generating mutant libraries for a variety of applications.
- State-of-the-Art Equipment
We use state-of-the-art technologies, including next-generation sequencing, to screen the mutant libraries that we generate and identify mutants with the desired phenotypes.
- Customization
- Rapid Turnaround Time:
We offer a fast turnaround time, which enables our clients to obtain their mutant libraries quickly and start their research projects in a timely manner.
- Cost-Effective Solutions
At CD Biosynsis, we offer a range of services for mutagenesis in strains, including chemical mutagenesis, site-directed mutagenesis, and random mutagenesis. Our services are tailored to the specific needs of our clients, and we work closely with them to develop mutagenesis strategies that are optimized for their research goals. We are able to generate high-quality mutant libraries that are enriched for mutants with the desired phenotypes. Contact us today to learn more about our mutagenesis in strains and how we can help optimize your microbial strains for maximum performance.
References
- Trovão, M., Schüler, L.M., et al. Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production. Marine Drugs 2022, 20, 440.
- Muteeb, G., Sen, R.. Random Mutagenesis Using a Mutator Strain. In: Braman, J. (eds) In Vitro Mutagenesis Protocols. Methods in Molecular Biology, 2010, vol 634. Humana Press, Totowa, NJ.
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