Trusted by Leading Research & Pharma Institutions

Custom sgRNA Libraries

Q: What delivery formats are available?

We offer flexible delivery options: plasmid DNA (standard), lentiviral particles (pre-packaged, ready-to-transduce), and E. coli glycerol stocks. Lentiviral format includes titer determination and detailed QC report. Custom packaging in AAV or adenovirus is also available.

Q: Can I customize the vector backbone?

Yes, we offer extensive vector customization options including: selection markers (puromycin, hygromycin, blasticidin), promoters (EF1a, CMV, UBC), fluorescent tags, inducible systems, and CRISPR modalities (CRISPRko, CRISPRa, CRISPRi). Our team can also clone into customer-provided vectors.

Q: What species are supported?

We support human, mouse, and rat genomes with validated sgRNA design databases. For other species, we can design custom sgRNAs based on your genomic sequences. Contact our team to discuss your specific requirements.

Q: Do you include control sgRNAs?

Yes, every library includes carefully designed control sgRNAs: non-targeting controls, safe harbor targeting controls, and essential gene positive controls. These are essential for screening data analysis and quality assessment.

Accelerate your CRISPR screening with custom sgRNA libraries designed for maximum efficiency and minimal off-target effects. Our proprietary synthesis platform delivers NGS-verified pooled libraries with industry-leading coverage and uniformity for genome-wide functional genomics studies.

NGS Verified

>99% Coverage

4-6 sgRNAs/gene

Learn More

Trusted by leading research and pharmaceutical institutions

MIT

Novartis

Johns Hopkins

Pfizer

Stanford

Roche

Why Choose Us

Proprietary synthesis platform

NGS-verified coverage >99%

4-6 optimized sgRNAs per gene

Expert consultation included

Genome-Wide Libraries

Up to 90,000 sgRNAs with complete coverage

Multiple CRISPR Modalities

CRISPRko, CRISPRa, CRISPRi support

Flexible Delivery Formats

Plasmid, lentivirus, or ready-to-transduce

Library Coverage

>99%

Service Overview

Comprehensive Custom sgRNA Library Solutions

Our proprietary synthesis platform delivers high-quality pooled sgRNA libraries with unmatched coverage and uniformity for CRISPR functional genomics screening.

Advanced Synthesis Platform

Our proprietary semiconductor-based electrochemical DNA synthesis platform enables no sequence restriction with top-notch library coverage and uniform distribution. We synthesize up to 90,000 unique sgRNA sequences in a single pool.

Up to 90,000 sgRNAs per library
Proprietary synthesis technology
No sequence restrictions

NGS Quality Verification

Every library undergoes comprehensive next-generation sequencing verification to ensure >99% coverage and uniform sgRNA distribution. Detailed QC reports are provided with every delivery.

>99% coverage guaranteed
Skew ratio <10
Complete NGS documentation

Optimized sgRNA Design

4-6 highly active sgRNAs per gene designed using validated algorithms for maximum on-target efficiency.

Multiple Modalities

Support for CRISPRko, CRISPRa, CRISPRi, and single-cell screening applications.

Flexible Delivery

Plasmid DNA, lentiviral particles, or ready-to-transduce formats available.

Technology Platform

Advanced Library Construction Technologies

Industry-leading synthesis and cloning platforms ensuring high-quality output for every project.

Proprietary Synthesis

Advanced semiconductor-based electrochemical DNA synthesis enabling high-complexity oligo pools without sequence restrictions.

No Limits High Fidelity

Gibson Assembly

Highly efficient Gibson Assembly cloning for seamless integration of sgRNA oligos into any lentiviral vector backbone.

High Efficiency scarless

NGS Validation

Comprehensive next-generation sequencing verification ensuring >99% coverage and uniform distribution of all sgRNAs.

QC Verified Documented

Optimized sgRNA Design

GC GC content 40-80%

Length 17-24 nt spacer length

Off-target Bioinformatic screening

sgRNAs/gene 4-6 guides per gene

Validation Empirically validated designs

Controls Non-targeting & positive controls

Specifications

Flexible Options for Diverse Needs

Comprehensive specifications to meet your research requirements.

Parameter	Standard Library	High-Complexity Library	Genome-Wide Library
Library Size	100 - 10,000 sgRNAs	10,000 - 50,000 sgRNAs	50,000 - 90,000 sgRNAs
sgRNAs per Gene	4-6	4-6	4-6
Coverage	>99%	>99%	>98%
Skew Ratio	<10	<10	<15
Delivery Format	Plasmid DNA	Plasmid / Lentivirus	Plasmid / Lentivirus
Species	Human, Mouse	Human, Mouse, Rat	Human, Mouse

Workflow

Streamlined Process from Design to Delivery

Our proven 5-step workflow ensures quality and efficiency at every stage.

Gene List

Submit your target genes or pathway

Design

Optimized sgRNA sequence design

Synthesis

High-fidelity oligo pool synthesis

Clone

Gibson Assembly into vector

QC & Delivery

NGS verification and shipment

Applications

Diverse Applications Across Biotechnology

Our custom sgRNA library services support research and development in multiple fields.

Genome-Wide CRISPR Screens

Comprehensive pooled sgRNA libraries covering the entire genome for unbiased functional genomics screens. Our libraries enable systematic identification of genes affecting cell viability, drug sensitivity, and biological processes.

Complete human and mouse genome coverage
Up to 90,000 sgRNAs in single pool
Optimized for essential gene identification
Compatible with MAGeCK analysis

90,000+

Maximum sgRNAs per library

Targeted Pathway Screening

Focused sgRNA libraries targeting specific gene families, pathways, or disease-related gene sets. Higher sgRNA density per gene provides improved statistical power for targeted studies.

Kinases, transcription factors, GPCRs
6-10 sgRNAs per gene for confidence
Custom gene sets available
Reduced screening cost

6-10

sgRNAs per gene

Drug Target Discovery

High-throughput CRISPR screening platforms for identifying and validating therapeutic targets. Our validated libraries accelerate the drug discovery pipeline from target identification to preclinical validation.

Synthetic lethality screening
Drug resistance mechanism discovery
Oncology target identification
Comprehensive analysis support

>90%

Hit validation rate

Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The coverage and uniformity exceeded our expectations. The NGS report was comprehensive and the library performed beautifully in our genome-wide screen. Highly recommended!"

Senior Scientist

Biotechnology Company

"Fast turnaround and excellent technical support throughout the project. The custom pathway library they designed helped us identify novel drug targets in record time."

Research Director

Pharmaceutical Company

"We've used this service for multiple CRISPR screening projects. Consistent quality and professional service every time. The expert consultation was particularly valuable."

Principal Investigator

Academic Research Institution

Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

187 Citations

Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library

Henkel L, Rauscher B, Schmitt B, Winter J, Boutros M. BMC Biology. 2020.

Novel empirically designed genome-scale sgRNA library with optimized on-target activity and reduced off-target effects for enhanced screening sensitivity.

View DOI

312 Citations

Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs

Jost M, Santos DA, Saunders RA, et al. Nature Biotechnology. 2020.

Systematic development of attenuated sgRNA libraries for titrating gene expression levels using deep learning-optimized mismatch rules.

View DOI

256 Citations

Improved sgRNA design for CRISPR screening

Doench JG, Fusi N, Sullender M, et al. Nature Biotechnology. 2020.

Optimization of sgRNA design algorithms and development of the Brunello library with significantly improved performance over earlier designs.

View DOI

178 Citations

Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing

Replogle JM, Bonnar JL, Phan NQ, et al. Nature Genetics. 2020.

Development of direct-capture Perturb-seq for pooled single-cell CRISPR screens with combinatorial perturbation libraries.

View DOI

203 Citations

Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning

Wang D, Zhang C, Wang B, et al. Nature Communications. 2019.

Deep learning-based sgRNA design optimization for high-fidelity Cas9 variants, significantly improving editing specificity.

View DOI

FAQ

Frequently Asked Questions

Find answers to common questions about our service.

We typically design 4-6 sgRNAs per gene, optimized using validated algorithms for maximum on-target efficiency and minimal off-target activity. For targeted pathway libraries, we can increase this to 6-10 sgRNAs per gene for enhanced statistical power.

Every library comes with comprehensive NGS quality control reports including: coverage (>99%), skew ratio (<10), empty vector content (<5%), and sgRNA distribution analysis. We guarantee these quality metrics or we'll remake the library at no additional cost.

We offer flexible delivery options: plasmid DNA (standard), lentiviral particles (pre-packaged, ready-to-transduce), and E. coli glycerol stocks. Lentiviral format includes titer determination and detailed QC report. Custom packaging in AAV or adenovirus is also available.

Yes, we offer extensive vector customization options including: selection markers (puromycin, hygromycin, blasticidin), promoters (EF1a, CMV, UBC), fluorescent tags, inducible systems, and CRISPR modalities (CRISPRko, CRISPRa, CRISPRi). Our team can also clone into customer-provided vectors.

We support human, mouse, and rat genomes with validated sgRNA design databases. For other species, we can design custom sgRNAs based on your genomic sequences. Contact our team to discuss your specific requirements.

Yes, every library includes carefully designed control sgRNAs: non-targeting controls, safe harbor targeting controls, and essential gene positive controls. These are essential for screening data analysis and quality assessment.

Ready to Start Your Project?

Get a customized quote for your Custom sgRNA Libraries Service project. Our experts will respond within 24 hours.

No obligation

24h response

Expert consultation

Custom sgRNA Libraries

Why Choose Us

Genome-Wide Libraries

Multiple CRISPR Modalities

Flexible Delivery Formats

Comprehensive Custom sgRNA Library Solutions

Advanced Synthesis Platform

NGS Quality Verification

Optimized sgRNA Design

Multiple Modalities

Flexible Delivery

Ready to Accelerate Your CRISPR Screening?

Advanced Library Construction Technologies

Proprietary Synthesis

Gibson Assembly

NGS Validation

Optimized sgRNA Design

Flexible Options for Diverse Needs

Streamlined Process from Design to Delivery

Gene List

Design

Synthesis

Clone

QC & Delivery

Diverse Applications Across Biotechnology

Genome-Wide CRISPR Screens

Targeted Pathway Screening

Drug Target Discovery

What Our Clients Say

Scientific Foundation

Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library

Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs

Improved sgRNA design for CRISPR screening

Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing

Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning

Frequently Asked Questions

Ready to Start Your Project?

Recommended Services

Related Resources

Request a Quote

Custom sgRNA Libraries

Why Choose Us

Genome-Wide Libraries

Multiple CRISPR Modalities

Flexible Delivery Formats

Comprehensive Custom sgRNA Library Solutions

Advanced Synthesis Platform

NGS Quality Verification

Optimized sgRNA Design

Multiple Modalities

Flexible Delivery

Ready to Accelerate Your CRISPR Screening?

Advanced Library Construction Technologies

Proprietary Synthesis

Gibson Assembly

NGS Validation

Optimized sgRNA Design

Flexible Options for Diverse Needs

Streamlined Process from Design to Delivery

Gene List

Design

Synthesis

Clone

QC & Delivery

Diverse Applications Across Biotechnology

Genome-Wide CRISPR Screens

Targeted Pathway Screening

Drug Target Discovery

What Our Clients Say

Scientific Foundation

Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library

Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs

Improved sgRNA design for CRISPR screening

Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing

Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning

Frequently Asked Questions

Ready to Start Your Project?

Recommended Services

Related Resources