Antibody gene synthesis is a sophisticated process that involves the creation of artificial antibody genes in a laboratory setting. It encompasses the design, assembly, and production of customized DNA sequences that encode antibodies with specific properties and functions. By leveraging advanced molecular biology techniques, scientists can synthesize antibody genes that are tailored to meet the unique requirements of various research, diagnostic, therapeutic, and drug discovery applications.
de novo antibody gene synthesis (T Tanaka,, et al.,2003)
Antibody gene synthesis offers a powerful tool for researchers and scientists to study and manipulate antibodies for a wide range of purposes. It allows for the production of antibodies with desired characteristics, such as increased affinity, improved stability, or enhanced specificity. This process enables the development of novel antibodies for the treatment of diseases, the detection of specific targets in diagnostic assays, and the exploration of new therapeutic targets in drug discovery.
The main uses of antibody gene synthesis include:
- Generate specific monoclonal antibodies: By synthesizing antibody gene sequences, monoclonal antibodies can be produced against specific antigens.
- Preparation of antibody library: Synthesize a large number of antibody gene sequences to establish an antibody library for screening and searching for antibodies with specific functions.
- Antibody engineering: By changing the sequence of antibody genes, the affinity, specificity, and other functions of antibodies can be altered.
Service Process
Case Studies
FAQs
Service Process
Our service process is designed to provide a seamless experience from start to finish. Here's an overview of how we handle antibody gene synthesis projects:
- Consultation: We understand the importance of understanding your specific requirements and goals for antibody gene synthesis. We begin by engaging in a thorough consultation where we take the time to listen to your needs and expectations. This allows us to have a clear understanding of what you aim to achieve with the synthesized antibody genes.
- Design and Optimization: Based on the information gathered during the consultation phase, our team of experts will design and optimize the DNA sequence for antibody gene synthesis. We carefully consider factors such as target specificity, functionality, and compatibility with your intended applications. Through our meticulous design and optimization process, we strive to create DNA sequences that yield high-quality synthesized antibody genes.
- Synthesis and Assembly: Once the DNA sequence design is finalized, we proceed with the synthesis and assembly of the antibody genes. Leveraging advanced molecular biology techniques, we synthesize the designed DNA sequence and assemble it into functional antibody genes. Throughout this process, we maintain strict quality control measures to ensure the accuracy and reliability of the synthesized antibody genes.
- Quality Control: Quality is of utmost importance to us. To ensure that the synthesized antibody genes meet the highest standards, we conduct rigorous quality control checks. This includes thorough testing and analysis to verify the functionality, specificity, and stability of the synthesized antibody genes. By adhering to stringent quality control protocols, we aim to deliver reliable and consistent results to our clients.
- Delivery: Once the quality control checks are successfully completed, we deliver the final synthesized antibody genes to you. As part of our commitment to transparency and comprehensive documentation, we provide you with detailed sequence information and other relevant documentation. This enables you to have a complete understanding of the synthesized antibody genes and facilitates their integration into your research, diagnostic, therapeutic, or drug discovery projects.
Our service process is designed with efficiency and precision in mind, allowing you to seamlessly incorporate the synthesized antibody genes into your scientific workflow.
Case Studies
The genes for the anti-EGFRvIII scFvs were cloned into the pBudCE4.1 dicistronic vector. The Ig k-chain signal sequence at the 5' end of each sequence allowed the secretion of the molecule in the culture media. "Knobs-into-holes" configuration and the two cysteine residues at the 3' end of each CH3 domain stabilize the construct. Sequence corresponding to a V5 epitope was cloned at the 3' end of one anti-EGFR scFvs and the other anti-EGFR scFv has Myc as well as a 6xHis tag at 3'end.
Schematic of the RAbDMvIII antibody (P Gupta, et al.,2010)
A) 30 ug of U87vIII and U87 wild type cell lysate loaded under reducing conditions on 4-20% SDS-PAGE. A) RAbvIII recognizes 145 kDa band which corresponds to the EGFRvIII and 45 kDa unspecific protein band. A) RAbDMvIII recognizes only 145 kDa protein band. B) RAbDMvIII recognizes 170 KDa protein band in 30 ug of A431 cell lysate (cells overexpressing EGFR) and 145 kDa band in 30 ug of HC2 cell lysate under reducing conditions. B) EGFR antibody recognizes equal amount of protein in both HC2 and A431 cell lysate under reducing conditions. C) RAbDMvIII under non reducing conditions detect only 145 kDa protein band whereas RAbvIII still recognizes 145 kDa and 170 kDa protein bands in non reducing conditions. C) Actin antibody under reducing condition shows equal amount of protein loaded in the wells.
Western Blot analysis of RAbDMvIII antibody (P Gupta, et al.,2010)
FAQs
We understand that you may have some questions regarding our antibody gene synthesis services. Here are answers to some frequently asked questions:
Q: How long does the antibody gene synthesis process take?
A: The turnaround time for antibody gene synthesis can vary depending on the complexity and quantity of the project. During the consultation phase, our team will provide you with an estimated timeline based on your specific requirements. We strive to provide efficient and timely delivery, ensuring that you can move forward with your projects without unnecessary delays.
Q: Can you synthesize antibody genes for different species?
A: Yes, our antibody gene synthesis services cover a wide range of species, including humans, mice, rats, and more. We understand that different research projects may require antibodies specific to different species. Please let us know your specific requirements, and we will accommodate accordingly to ensure that the synthesized antibody genes align with the desired species.
Q: What are the applications of antibody gene synthesis?
A: Antibody gene synthesis has numerous applications across various fields. It plays a crucial role in research, enabling scientists to study specific targets, investigate diseases, and develop new therapies. Furthermore, antibody gene synthesis is utilized in diagnostics to detect specific markers or antigens, as well as in drug discovery to identify potential therapeutic targets. The ability to produce custom antibodies through gene synthesis opens up a world of possibilities for advancing scientific knowledge and improving human health.
Q: How can I request a quote for antibody gene synthesis?
A: Requesting a quote for our antibody gene synthesis services is easy. Simply contact our team via email or phone, and we will be happy to assist you. We understand that each project is unique, and we will work closely with you to provide a customized solution based on your specific needs. Our team is dedicated to delivering high-quality antibody gene synthesis services that align with your goals and expectations.
We look forward to the opportunity to collaborate with you on your antibody gene synthesis projects. Contact us today to discuss your requirements and explore how our services can support your research, diagnostic, therapeutic, or drug discovery endeavors.
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