Antibody Gene Synthesis

Rapid Synthesis & Cloning of Antibody Heavy and Light Chain Sequences

Accelerate your antibody discovery pipeline with precision gene synthesis tailored for therapeutic and research applications.

Our antibody gene synthesis service delivers sequence-verified, custom-cloned heavy and light chain DNA constructs in as little as 6 days. Optimize your antibody expression with codon optimization, any custom vector, and rigorous QC — all designed to support your downstream protein production and functional studies.

100% sequence-verified clonal DNA
Custom vector construction for any expression system
Codon optimization for mammalian, E. coli, or yeast hosts
Rapid turnaround from sequence submission to lyophilized DNA

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Service Highlights

End-to-end support from sequence optimization to final clone.

Synthesis and cloning of heavy and light chain genes
Any custom vector accepted
Sequence verification by Sanger sequencing
Lyophilized DNA and glycerol stock deliverables

Therapeutic antibody discovery

Hybridoma & phage display projects

Recombinant antibody production

Why Antibody Gene Synthesis?

Precision DNA Construction for Next-Generation Antibodies

Antibody sequence optimization and vector construction are critical for efficient expression and functional activity. Our service provides the exact DNA constructs you need to move from candidate to characterization.

Complete Synthesis & Cloning

We synthesize your antibody heavy and light chain variable regions (VH, VL) and clone them into your chosen expression vector. Whether you need single-chain variable fragments (scFv), Fab fragments, or full-length IgG, we deliver sequence-verified plasmid DNA ready for transfection.

Synthesis of VH, VL, full-length heavy and light chains
Cloning into any custom vector (mammalian, bacterial, yeast)
100% sequence verification by Sanger

Sequence Optimization

Codon optimization can enhance expression levels in your host system. We analyze and optimize your antibody gene sequences to match the codon bias of CHO, HEK293, E. coli, or other expression hosts, while preserving the encoded amino acid sequence.

Codon optimization for mammalian, E. coli, yeast
Removal of cryptic splice sites and rare codons
GC content and secondary structure balancing

Therapeutic Antibody Development

Generate high-quality DNA constructs for lead candidate expression, screening, and optimization.

Synthesis of multiple variants (e.g., affinity maturation)
Compatible with transient and stable expression systems

Diagnostic & Research Reagents

Produce antibody genes for ELISA, Western blot, flow cytometry, and other immunoassays with consistent quality.

Custom epitope tags and linkers included
Small-scale and large-scale production options

Hybridoma & Phage Display Output

Convert hybridoma or phage display-derived sequences into expression-ready constructs for recombinant production.

Direct cloning from hybridoma or phage display vectors
Humanization or chimerization services available

Technology & Workflow

How We Deliver Your Antibody Genes

Our process integrates advanced synthesis chemistry, automated cloning, and rigorous quality control to ensure every construct meets your specifications.

Sequence Submission & Optimization

Provide your antibody heavy and light chain sequences (or those of your variable regions). We perform codon optimization and eliminate problematic motifs while preserving the native amino acid sequence.

Codon Optimization

Human, mouse, CHO, HEK293, E. coli, yeast codon tables
GC content optimization to avoid secondary structure

Sequence Analysis

Removal of internal restriction sites and repeats
Prediction of mRNA stability and translation efficiency

Gene Synthesis & Cloning

We synthesize the optimized DNA sequences using high-fidelity enzymatic synthesis and clone them into your chosen vector. Simultaneous synthesis of both heavy and light chain constructs is available.

Synthesis

High-fidelity, error-free synthesis for up to 3 kb
Parallel synthesis of multiple constructs

Cloning

Any custom vector accepted (mammalian, lentiviral, bacterial)
Restriction enzyme or Gibson Assembly® compatible

Quality Control & Delivery

Every clone is sequence-verified to 100% accuracy. We provide lyophilized DNA, glycerol stock, and full documentation including sequencing chromatograms.

Sequence Verification

Full-length Sanger sequencing of both strands
Alignment report confirming 100% identity

Deliverables

Lyophilized plasmid DNA (≥4 µg)
Glycerol stock of E. coli clone
Electronic sequence and QC data

Supported Expression Systems

Mammalian (CHO, HEK293, NS0, SP2/0)
E. coli (periplasmic expression)
Yeast (P. pastoris, S. cerevisiae)
Insect cell (baculovirus)

Customizable Options

Signal peptide optimization
Epitope tags (His, HA, FLAG, Myc, etc.)
Flexible linker sequences for scFv or bispecific formats
Multiple cloning site variations

Comparison

Antibody Gene Synthesis Service vs. Traditional Cloning

Our service eliminates the time-consuming steps of PCR amplification, restriction digestion, and ligation, delivering sequence-verified constructs in a fraction of the time.

Feature	Our Antibody Gene Synthesis Service	Traditional Cloning from cDNA
Turnaround Time	As few as 6 days for standard constructs	2–4 weeks typically
Sequence Verification	100% sequence-verified by Sanger	Often limited to restriction digest or partial sequencing
Codon Optimization	Included for any host system	Not available
Custom Vector Cloning	Any vector accepted	Limited to vector with compatible restriction sites
Multiple Constructs	Parallel synthesis of heavy and light chains	Sequential cloning required
Error-Free Synthesis	Enzymatic synthesis with proofreading	PCR errors possible

Workflow

From Sequence to Clone in a Few Simple Steps

Our streamlined process ensures you receive sequence-verified antibody gene constructs without unnecessary delays.

1. Submit Sequences

Provide your antibody heavy and light chain sequences (or variable regions) via our online portal. Include your desired vector, expression host, and any special requirements (e.g., tags, linkers).

2. Sequence Optimization

Our team analyzes your sequences for codon optimization, removes problematic motifs, and ensures compatibility with your chosen expression system. You receive an optimization report for approval.

3. Synthesis & Cloning

We synthesize the optimized DNA using high-fidelity enzymatic synthesis and clone it into your chosen vector. Both heavy and light chain constructs are processed simultaneously.

4. Quality Control

Every clone undergoes full-length Sanger sequencing of both strands. We verify 100% sequence identity and provide an alignment report. Only clones passing QC are released.

5. Delivery

You receive lyophilized plasmid DNA, a glycerol stock of the transformed E. coli clone, and electronic data including sequencing chromatograms and QC certificates.

Applications

Where Antibody Gene Synthesis Makes a Difference

From therapeutic discovery to diagnostic reagent production, our service supports a wide range of antibody development workflows.

Accelerate Lead Candidate Generation

In therapeutic antibody development, speed and accuracy are paramount. Our gene synthesis service enables you to quickly convert hybridoma-derived sequences, phage display outputs, or in silico-designed antibodies into expression-ready constructs. Codon optimization for CHO or HEK293 cells can improve expression yields, while simultaneous synthesis of heavy and light chain genes reduces the time from candidate identification to functional testing.

Parallel synthesis of heavy and light chains for IgG, scFv, Fab
Codon optimization for mammalian expression systems
Compatible with transient and stable cell line generation

Reliable DNA for Research Reagents

Single-vector or dual-vector expression systems
Custom tags (His, Fc, FLAG) for purification
Small-scale to large-scale synthesis options

Rapid Cloning for Assay Development

Direct cloning from hybridoma or single-cell PCR
Optimization for E. coli or mammalian expression
Sequence verification eliminates cloning errors

FAQ

Antibody Gene Synthesis — Common Questions

Find answers to frequently asked questions about our service, including turnaround, sequence requirements, and customization options.

We synthesize antibody gene fragments from as small as 100 bp up to 3 kb. For typical antibody variable regions (VH, VL ~350 bp each) or full-length heavy and light chains (1.5–2 kb), our synthesis process is fully compatible. If you need larger constructs, please contact us to discuss options.
Yes, we accept any custom vector you provide. We can clone your antibody gene using restriction enzyme-based or Gibson Assembly® methods. Please provide at least 1 µg of purified vector DNA and a sequence map. We will confirm compatibility before proceeding.
Yes, codon optimization is included in our service. We analyze your antibody sequence and optimize it for the expression host you specify (e.g., CHO, HEK293, E. coli, yeast). The optimization preserves the amino acid sequence while removing rare codons, cryptic splice sites, and secondary structures that could reduce expression.
Every clone is verified by full-length Sanger sequencing of both strands. We compare the sequencing data to the reference sequence and provide an alignment report confirming 100% sequence identity. Only clones that pass this rigorous QC are shipped.

Scientific Literature

References

Ganesh K, Neuberger MS. The relationship between hypothesis and experiment in unveiling the mechanisms of antibody gene diversification. FASEB J 2011 Apr. PubMed
Willis JR, Briney BS, DeLuca SL. Human germline antibody gene segments encode polyspecific antibodies. PLoS Comput Biol 2013 Apr. PubMed
Hsia HE, Zanini C, Simonneau C. Improved targeted delivery of antisense oligonucleotide with an antibody mask. Nucleic Acids Res 2025 Oct 28. PubMed
Zhou X, Ma F, Xie J. Diverse immunoglobulin gene usage and convergent epitope targeting in neutralizing antibody responses to SARS-CoV-2. Cell Rep 2021 May 11. PubMed
Brücher D, Franc V, Smith SN. Malignant tissues produce divergent antibody glycosylation of relevance for cancer gene therapy effectiveness. MAbs 2020 Jan-Dec. PubMed

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