Trusted by Leading Research & Pharma Institutions

Antibody Gene Synthesis Services

Accelerate your antibody discovery and development with custom antibody gene synthesis. From variable regions to full-length antibodies, we deliver sequence-verified genes optimized for high-level expression in mammalian systems.

NGS Verified

Codon Optimized

100% Sequence Accuracy

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Trusted by leading research and pharmaceutical institutions

MIT

Pfizer

Stanford

Roche

Johns Hopkins

Merck

Why Choose Us

NGS-verified sequence accuracy

Codon optimization for high expression

Mammalian, CHO, and E. coli vectors

Complete QC documentation

Variable Region Genes

VH, VL, scFv, and Fab fragments

Full-Length Antibodies

IgG1, IgG2, IgG4, and bispecifics

Fc Fusion Proteins

Optimized for stability and yield

Success Rate

99.5%

Service Overview

Complete Antibody Gene Synthesis Solutions

From antibody variable regions to full-length therapeutic antibodies, our synthesis platform delivers sequence-perfect genes ready for expression.

Variable Region Synthesis

High-quality synthesis of antibody variable regions including VH, VL, scFv, and Fab fragments. Each gene is designed with optimal codons for your expression system of choice.

Heavy chain variable region (VH)
Light chain variable region (VL)
Single-chain variable fragment (scFv)
Antigen-binding fragment (Fab)

Full-Length Antibody Genes

Complete antibody genes including constant regions for IgG1, IgG2, and IgG4 isotypes. Includes signal peptide, hinge region, and Fc portion for proper secretion and function.

IgG1, IgG2, IgG4 isotypes
Signal peptide for secretion
Human and murine constant regions
Bispecific antibody formats

NGS Verification

Every antibody gene verified by next-generation sequencing for 100% sequence accuracy.

Codon Optimization

Proprietary algorithms optimize codons for CHO, HEK293, and E. coli expression systems.

Multiple Formats

Delivered as plasmid, linear fragment, or cloned into expression vectors.

Technology Platform

Advanced Synthesis Technologies

Our synthesis platform combines industry-leading DNA synthesis with proprietary verification methods.

High-Fidelity Synthesis

Proprietary synthesis platform achieving 99.9% coupling efficiency for long gene sequences with reduced error rates.

99.9% Accuracy Proprietary

NGS Verification

Every antibody gene undergoes next-generation sequencing verification to ensure sequence perfection before delivery.

Illumina 100% Verified

Expression Optimization

Codon optimization algorithms specifically designed for antibody genes, considering mRNA stability and folding.

CHO HEK293 E. coli

Quality Control

NGS Next-gen sequencing verification

SEQ Sanger sequencing confirmation

QC Restriction digest and gel analysis

COA Certificate of Analysis included

Delivery Formats

PLASMID Miniprep to maxiprep in standard vectors

LINEAR Linear dsDNA fragment ready for cloning

EXPRESS Pre-cloned in expression vectors

FREEZE Transient transfection-ready DNA

Specifications

Flexible Options for Diverse Needs

Comprehensive specifications to meet your antibody development requirements.

Parameter	Variable Region	scFv/Fab	Full-Length IgG
Gene Length	400 - 500 bp	700 - 1,500 bp	1,400 - 1,500 bp
Coupling Efficiency	99.9%	99.9%	99.9%
Turnaround Time	10-14 business days	12-16 business days	14-18 business days
Scale Range	1 - 10 μg	1 - 10 μg	1 - 5 mg
Verification	NGS + Sanger	NGS + Sanger	NGS + Sanger
Delivery Format	Plasmid or Linear	Plasmid or Linear	Plasmid (expression ready)

Workflow

Streamlined Process from Design to Delivery

Our proven 5-step workflow ensures quality and efficiency at every stage.

Order

Submit sequence and specifications

Design

Codon optimization and sequence check

Synthesis

High-fidelity gene synthesis

QC

NGS and Sanger verification

Delivery

Plasmid with QC report

Applications

Diverse Applications Across Biotechnology

Our synthesis services support research and development in multiple fields.

Antibody Discovery

Accelerate your antibody discovery pipeline with rapid gene synthesis for hybridoma screening, phage display, and single B cell cloning workflows.

Rapid gene synthesis for hit validation
Humanization and affinity maturation
scFv and Fab library construction
Multi-specific antibody formats

99.5%

Sequence accuracy rate

Therapeutic Development

Support your therapeutic antibody development with GMP-quality gene synthesis, optimized for high-yield production in mammalian cell systems.

CHO expression-optimized sequences
Fc engineering for half-life extension
Bispecific antibody formats
ADC linker attachment sites

5 g/L

Typical CHO expression titer

Diagnostic Applications

High-quality antibody genes for diagnostic assay development, including sandwich ELISA, lateral flow, and immunofluorescence applications.

Sandwich ELISA pairs
Lateral flow assay antibodies
Immunofluorescence (IF/IHC)
Recombinant protein production

12+

Applications supported

Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The NGS verification gives us complete confidence in our antibody sequences. We've eliminated sequencing errors from our discovery workflow and accelerated our timeline significantly."

Senior Scientist

Biopharmaceutical Company

"The codon optimization for CHO cells has improved our expression titers dramatically. The team provided excellent technical support throughout the project."

Research Director

Academic Research Institution

"We've been using this service for our bispecific antibody development. The quality and accuracy have been consistent across multiple projects. Highly recommended."

Principal Investigator

Therapeutic Antibody Company

Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

85 Citations

A rapid cell-free expression and screening platform for antibody discovery

Bhamidi V, et al. Nature Communications. 2023.

Development of a workflow combining cell-free DNA template generation, cell-free protein synthesis, and antibody fragment binding measurements in hours rather than weeks.

View DOI

62 Citations

Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening

Segal-Halevist D, et al. Nature Biomedical Engineering. 2023.

Discovery of low-nanomolar nanobodies from yeast display libraries and high-picomolar single-chain antibody fragments from unselected synthetic libraries.

View DOI

28 Citations

Highly reliable GIGA-sized synthetic human therapeutic antibody library construction

Huang C-Y, et al. Frontiers in Immunology. 2023.

A highly reliable GIGA-scale synthetic human therapeutic antibody library construction method, providing a new technology platform for cancer immunotherapy.

View DOI

42 Citations

A Cell-free Expression Pipeline for the Generation and Functional Characterization of Nanobodies

Haueis M, et al. Frontiers in Bioengineering and Biotechnology. 2022.

Synthesis of functional nanobodies in a mammalian CHO cell lysate system, enabling on-demand synthesis within 3 hours without requiring a translocation step.

View DOI

45 Citations

Predicting antibody binders and generating synthetic antibodies using deep learning

Akbar R, et al. mAbs. 2022.

Using deep learning models to predict antibody binding sites and generate synthetic antibodies, providing new computational methods for antibody engineering and discovery.

View DOI

FAQ

Frequently Asked Questions

Find answers to common questions about our service.

Our antibody gene synthesis includes: codon-optimized DNA sequence, gene synthesis using proprietary high-fidelity platform, NGS verification for 100% sequence accuracy, Sanger sequencing confirmation, restriction digest QC, Certificate of Analysis, and delivery as plasmid DNA or linear fragment. For full-length antibodies, genes are pre-cloned into expression vectors ready for transient or stable transfection.

Every antibody gene undergoes dual verification: next-generation sequencing (Illumina) to confirm the entire gene sequence, followed by Sanger sequencing as an additional confirmation step. This two-tier verification ensures 100% sequence accuracy before delivery, eliminating common errors in traditional gene synthesis.

We support codon optimization for multiple expression systems including CHO (Chinese hamster ovary), HEK293 (human embryonic kidney), and E. coli. For therapeutic applications, we optimize for high-yield CHO expression to maximize protein productivity. Signal peptides and secretion signals are included based on your target system.

Standard turnaround times are: Variable region genes (VH/VL): 10-14 business days; scFv/Fab fragments: 12-16 business days; Full-length IgG antibodies: 14-18 business days. Express services are available for urgent projects with reduced timelines. Rush service pricing applies.

Yes, we offer antibody humanization as an additional service. Our humanization process maintains the original antigen-binding affinity while reducing immunogenicity by grafting complementarity-determining regions (CDRs) onto human framework sequences. Contact us for a custom quote based on your specific antibody sequences.

We offer multiple delivery formats: plasmid DNA in standard bacterial vectors (miniprep to maxiprep scales), linear double-stranded DNA fragment ready for your cloning workflow, or pre-cloned into expression vectors including pcDNA, pCMV, and proprietary mammalian expression vectors. Expression-ready formats are ideal for immediate transient transfection experiments.

Ready to Start Your Project?

Get a customized quote for your Antibody gene synthesis service project. Our experts will respond within 24 hours.

No obligation

24h response

Expert consultation

Antibody Gene Synthesis Services

Why Choose Us

Variable Region Genes

Full-Length Antibodies

Fc Fusion Proteins

Complete Antibody Gene Synthesis Solutions

Variable Region Synthesis

Full-Length Antibody Genes

NGS Verification

Codon Optimization

Multiple Formats

Ready to Start Your Antibody Project?

Advanced Synthesis Technologies

High-Fidelity Synthesis

NGS Verification

Expression Optimization

Quality Control

Delivery Formats

Flexible Options for Diverse Needs

Streamlined Process from Design to Delivery

Order

Design

Synthesis

QC

Delivery

Diverse Applications Across Biotechnology

Antibody Discovery

Therapeutic Development

Diagnostic Applications

What Our Clients Say

Scientific Foundation

A rapid cell-free expression and screening platform for antibody discovery

Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening

Highly reliable GIGA-sized synthetic human therapeutic antibody library construction

A Cell-free Expression Pipeline for the Generation and Functional Characterization of Nanobodies

Predicting antibody binders and generating synthetic antibodies using deep learning

Frequently Asked Questions

Ready to Start Your Project?

Recommended Services

Related Resources

Request a Quote

Antibody Gene Synthesis Services

Why Choose Us

Variable Region Genes

Full-Length Antibodies

Fc Fusion Proteins

Complete Antibody Gene Synthesis Solutions

Variable Region Synthesis

Full-Length Antibody Genes

NGS Verification

Codon Optimization

Multiple Formats

Ready to Start Your Antibody Project?

Advanced Synthesis Technologies

High-Fidelity Synthesis

NGS Verification

Expression Optimization

Quality Control

Delivery Formats

Flexible Options for Diverse Needs

Streamlined Process from Design to Delivery

Order

Design

Synthesis

QC

Delivery

Diverse Applications Across Biotechnology

Antibody Discovery

Therapeutic Development

Diagnostic Applications

What Our Clients Say

Scientific Foundation

A rapid cell-free expression and screening platform for antibody discovery

Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening

Highly reliable GIGA-sized synthetic human therapeutic antibody library construction

A Cell-free Expression Pipeline for the Generation and Functional Characterization of Nanobodies

Predicting antibody binders and generating synthetic antibodies using deep learning

Frequently Asked Questions

Ready to Start Your Project?

Recommended Services

Related Resources