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Strain Engineering for Alanine

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CD Biosynsis can help our customers develop synthetic biology approaches to construct microbial cell factories or deign cell-free enzymatic cascades for efficient alanine production and develop promising methods for industrial production of alanine. Our scientists are committed to accelerating technological innovation in the large-scale and commercial production of alanine.

Advantages of Synthetic Biology-Driven Alanine Production

Alanine with the molecular formula C3H7NO2 is a nonessential amino acid and the key building block of many cellular proteins. With the increasing problem of fossil fuel depletion and environmental pollution caused by chemical industry, synthetic biology strategies in the biological production of alanine have attracted more attention. The power of synthetic biology allows the replacement of fossil fuels with renewable feedstocks, realizing a flexible feedstock concept. This could drive the transformation of alanine production towards green biomanufacturing and help reduce energy consumption, pollution, and production costs. The development of synthetic biology strategies for alanine production can facilitate its wide applications in various fields such as food, feed, medicine, and environment, and drive growth of the global alanine market.

Figure 1. Two production routes for L-alanine production. (A) Enzymatic process using petroleum-based raw materials; (B) fermentation process using renewable biomass. (Liu P, et al., 2022)Figure 1. Two production routes for L-alanine production. (A) Enzymatic process using petroleum-based raw materials; (B) fermentation process using renewable biomass. (Liu P, et al., 2022)

What We Provide

Leveraging our powerful synthetic biology platform, CD Biosynsis can provide our customers with custom synthetic biology services to help them develop effective strategies to achieve efficient biosynthesis of alanine.

Featured Services

  • Identification, characterization, and expression of key enzymes such as L-alanine dehydrogenase (ALD) in the host strains for efficient alanine production.
  • Integration or deletion of genes involved in the competitive metabolic pathways to overcome challenges posed by the formation of coproducts and increase alanine purity.
  • Rerouting of carbon flux to reduce carbon loss and enrich the metabolic flux for enhanced alanine biosynthesis.
  • Design and characterization of novel alanine biosynthetic pathway composed of highly specialized enzymes.


  • Alanine-producing microorganisms.
  • High-quality L-alanine, D-alanine, DL-alanine, and β-alanine.

How We Can Help

CD Biosynsis has a team of scientists specializing in synthetic biology to help our customers develop customized strategies for high-yield production of alanine. Using our advanced synthetic biology platform, we are committed to developing efficient, eco-friendly, sustainable, and cost-effective process for producing alanine to meet growing market demands.

Enzyme Design for Alanine Synthesis

By combining computational design, in silico screening and experimental validation, we have the ability to determine the enzymes of alanine synthesis and introduce new functions into these enzymes for efficient alanine production. We can redesign enzyme active sites to expand their substrate scope and convert highly substrate-specific enzymes into useful general biocatalysts for alanine synthesis.

Engineering of Microorganisms for Alanine Production

We can develop effective microbial engineering strategies to achieve enhanced production of alanine in different microbial strains using low-cost substrates. Our efforts are currently focused on the metabolic engineering of Escherichia coli, Lactococcus lactis, Arthrobacter oxydans, Bacillus sphaericus, Clostridium sp., Zymomonas mobilis, and Corynebacterium glutamicum for enhanced alanine synthesis. If you are interested in other microorganisms, please fill out the online inquiry form and tell us more about your project.

Cell-Free Enzymatic Cascade Design

Establishing cell-free enzymatic reaction cascade in the absence of a regulatory cell environment allows for the control of metabolic pathways and simplification of alanine production processes. With expertise in cell-free synthetic biology, we can help customers select highly active enzymes, design and establish multi-step metabolic pathways, and optimize the production process to enable the preparative-scale production of alanine directly from substrates such as glucose.

Applications of Alanine

With extensive experience in customer-focused production of alanine, CD Biosynsis has the ability to deliver high-quality products to meet the needs of our customers in different industries.

  • Widely used as a precursor of many significant industrial chemicals, such as MGDA and vitamin B6,
  • Used as an ingredient or intermediate for pharmaceutical products.
  • Used as a nutrition supplement, sweetener, fragrance ingredient, flavor enhancer, and sour corrective agent in food and feed.
  • Used as an antistatic agent, hair conditioning agent, skin-conditioning agent in cosmetic and personal care products.
  • Used as a pre- and postoperative nutrition therapy.

Want to Learn More?

As a rapidly growing synthetic biology company, CD Biosynsis is committed to helping our customers meet the growing and evolving demand for amino acid production. All of our deliverables will undergo a rigorous quality testing process to ensure the quality and reliability and can be delivered on time. If you are interested in our services or have any further questions, please do not hesitate to contact us.


  1. Liu P, et al. Metabolic engineering of microorganisms for L-alanine production. Journal of Industrial Microbiology and Biotechnology, 2022, 49(2): kuab057.
  2. Wang L, et al. Advances in biotechnological production of β-alanine. World Journal of Microbiology and Biotechnology, 2021, 37(5): 1-11.

Please note that all services are for research use only. Not intended for any clinical use.

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