Introduction of Plant Virus-Based Vector Systems
Plant virus-based vector systems have emerged as an efficient and practical tool for the production of recombinant proteins in plants. This technology has revolutionized the field of synthetic biology and has been widely adopted for the production of high-value proteins, such as vaccines, antibodies, and enzymes.
CD Biosynsis is a leading company in the field of synthetic biology, with years of experience in the industry. We have developed a strong expertise in the use of plant virus-based vector systems for the expression of recombinant proteins in plants.
Figure 1: TMV based plant-derived vaccine for Non-Hodgkins Lymphoma. (Hefferon K. L. 2012)
Our Services for Plant Virus-Based Vector Systems
Vector Design and Construction
Vector design and construction is a critical step in the production of recombinant proteins using plant virus-based vector systems. CD Biosynsis has a team of experts who can design and construct the appropriate vector for your specific protein expression needs. Our process involves:
- Analyzing the protein of interest and determining the most appropriate plant virus-based vector system for the expression of the protein.
- Designing the vector construct to include the gene of interest, a promoter, and other regulatory elements necessary for expression.
- Cloning the vector construct into the appropriate plant virus-based vector system.
Agroinfiltration
Agroinfiltration is a simple and effective method for the rapid production of recombinant proteins in plants using plant virus-based vector systems. CD Biosynsis offers agroinfiltration services for the transient expression of recombinant proteins in plants. Our process involves:
- Growing the plants in a controlled environment.
- Preparing the Agrobacterium strain containing the plant virus-based vector system.
- Infiltrating the Agrobacterium into the plant leaves using a syringe or vacuum infiltration.
Magnifection
Magnifection is a high-yield process that relies on Agrobacterium as a vector to deliver DNA copies of one or more viral RNA replicons to plant cells. CD Biosynsis offers magnifection services for the large-scale production of recombinant proteins in plants using plant virus-based vector systems. Our process involves:
- Growing the plants in a controlled environment.
- Preparing the Agrobacterium strain containing the plant virus-based vector system.
- Co-infecting the plants with Agrobacterium containing the plant virus-based vector system and another Agrobacterium strain containing the replication machinery.
- Harvesting the plants and extracting the recombinant protein.
Analytical Services
CD Biosynsis provides analytical services for the characterization of recombinant proteins produced using plant virus-based vector systems. Our analytical services include protein quantification, purity analysis, and functional assays. Our process involves:
- Quantifying the amount of recombinant protein produced.
- Analyzing the purity of the recombinant protein using techniques such as SDS-PAGE and HPLC.
- Testing the functional activity of the recombinant protein using appropriate assays.
Our Advantages
- High Yield: Plant virus-based vector systems allow for the large-scale production of recombinant proteins in plants, resulting in high yields of the desired product.
- Fast Manufacturing: The use of plant virus-based vector systems allows for the rapid production of recombinant proteins in plants, making it an ideal technology for the production of time-sensitive products, such as vaccines.
- Cost-Effective: Plant virus-based vector systems are a cost-effective alternative to conventional protein production methods, such as mammalian cell culture or microbial fermentation.
- Safe: Plant virus-based vector systems do not incorporate the gene of interest into the host plant genome, making it a safe technology for the production of recombinant proteins in plants.
CD Biosynsis offers a comprehensive range of services for the production of recombinant proteins using plant virus-based vector systems. Our services include vector design and construction, agroinfiltration, magnifection, and analytical services. Our technology offers several advantages over conventional protein production methods, such as high yield, fast manufacturing, cost-effectiveness, and safety. Contact us today to learn more about our services and how we can help you with your protein expression needs.
References
- Hefferon K. L. Plant virus expression vectors set the stage as production platforms for biopharmaceutical proteins. Virology, 2012, 433(1): 1-6.
- Hefferon K. L. Plant Virus Expression Vectors: A Powerhouse for Global Health. Biomedicines, 2017, 5(3): 44.
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