CD Biosynsis is committed to helping our customers develop synthetic biology strategies to improve the production of propanediol (PDO), reduce production costs, and accelerate the industrial production of bio-based PDO. Our scientists with expertise in synthetic biology chassis development, metabolic engineering, and fermentation biology have the ability to develop more efficient microbial cell factories that can produce 1,2-PDO or 1,3-PDO from inexpensive feedstocks.
Advantages of Synthetic Biology-Driven Propanediol Production
Propanediol (PDO) with the molecular formula C3H8O2 is an important platform chemical and a widely studied value-added product with high industrial demand. Due to the depletion of natural resources and the increase of government regulations, there is an urgent need to make a transition from the chemical synthesis of PDO to more economical and environmentally sustainable bio-based production. However, microbial production of PDO is largely limited by low efficiency and a number of factors hinder the commercialization process. Advances in synthetic biology and metabolic engineering hold promise for overcoming these obstacles. New strategies and tools of synthetic biology offer an attractive and efficient route for PDO production. Industrialization of synthetic biology-driven microbial production process is also an effective way to reduce the cost of PDO production.
Figure 1. Natural (A) and engineered metabolic pathways (B–D) enabling biosynthesis of 1,3-propanediol (PDO). (Frazão C J R, et al., 2019)
What We Provide
Leveraging our powerful synthetic biology platform, CD Biosynsis can provide our customers with custom synthetic biology services to help them develop effective strategies to achieve efficient biosynthesis of propanediol.
Featured Services
- Enhancement of major metabolic pathways for PDO production by overexpressing endogenous genes and introducing heterologous genes.
- Redistribution of carbon flux and enhancement of substrate utilization range in engineered microorganisms.
- Development of efficient strategies for the knockout of genes encoding competing pathways to reduce by-products.
- Engineering of non-natural PDO synthetic metabolic pathways to reduce the accumulation of toxic intermediates and improve PDO production.
Key Strategies and Technologies
Deliverables
- Propanediol-producing microorganisms.
- High-quality 1,3-propanediol and 1,2-propanediol.
How We Can Help
CD Biosynsis can develop effective synthetic biology approaches to help our customers achieve efficient PDO production in a wide range of engineered microorganisms. Our scientists have the ability to accelerate the application of microbial chassis and PDO biosynthetic pathways in industrialized manufacturing.
Discovery and Engineering of Microorganisms for PDO Production
We can help discover natural microorganisms with the capability to produce PDO or we can develop engineered microbial strains with high PDO productivity. A collection of microbial strains can be used for PDO production is listed below. If you can't find the strain you are interested in, please fill out the online inquiry form and tell us more about your project.
| Bacteroides ruminicola |
Salmonella typhimurium |
Klebsiella pneumoniae |
Clostridium butyricum |
Clostridium pasteurianum |
| Clostridium thermobutyricum |
Clostridium sphenoides |
Clostridium strain AK-1 |
Lactobacillus buchneri |
Escherichia coli |
| Corynebacterium glutamicum |
Synechococcus elongatus PCC 7942 |
Synechocystis sp. PCC6803 |
Salmonella typhimurium |
Lactobacillus brevis |
| Candida polymorpha |
Pichia robertsii |
Saccharomyces cerevisiae |
Prototheca wickerhamii |
Torulopsis famata |
Design and Engineering of Biosynthetic Pathways for PDO Production
Our scientists have been investigating biosynthetic pathways and key enzymes for PDO production such as deoxyhexose pathway, methylglyoxal pathway, lactate pathway, and glycerol disproportionation pathway. We can develop synthetic biology tools and strategies to improve PDO production and reduce costs by enhancing major metabolic pathways, redistributing carbon flux, enhancing substrate utilization, reducing the accumulation of toxic intermediates, and engineering non-natural PDO synthetic pathways. We have the capability to construct cell factories and develop bioprocesses for more-efficient industrial bio-production of PDO.
Applications of Propanediol
CD Biosynsis is committed to increasing the production of bio-based PDO using advanced synthetic biology technologies to meet the growing demand in various applications.
- Used as an antifreeze in the food, chemical, and pharmaceutical industries.
- Used as a preservative and solvent in food, cosmetics, personal care products and pharmaceutical products.
- Used as a building block in the production of polymers, such as polytrimethylene terephthalate (PTT) and polyurethane.
- Used in the production of various industrial products, such as coatings, adhesives, laminates, plastics, film, and fiber.
- Used as an anti-mold agent and an emulsifier in food products.
- Used as a raw material of surfactant, plasticizer, emulsifier, demulsifier, moisturizer, and airplane deicing fluid.
Want to Learn More?
As a rapidly growing synthetic biology company, CD Biosynsis is committed to helping our customers meet the growing and evolving demand for bio-based chemical production. All of our deliverables will undergo a rigorous quality testing process to ensure the quality and reliability and can be delivered on time. If you are interested in our services or have any further questions, please do not hesitate to contact us.
References
- Tao Y, et al. A comprehensive review on microbial production of 1, 2-propanediol: micro-organisms, metabolic pathways, and metabolic engineering. Biotechnology for Biofuels, 2021, 14(1): 1-16.
- Frazão C J R, et al. Construction of a synthetic pathway for the production of 1, 3-propanediol from glucose. Scientific reports, 2019, 9(1): 1-12.
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