Trusted by Leading Research & Pharma Institutions

Prime Gene Editing Services

Accelerate your precision genome engineering with our comprehensive Prime Editing solutions. From chemically synthesized pegRNAs to PE mRNA, we deliver research-grade and clinical-grade components for seamless "search-and-replace" genome editing without double-strand breaks.

Up to 266 nt

>60% Efficiency

RUO to GMP

Learn More

Trusted by leading research and pharmaceutical institutions

Stanford

Pfizer

MIT

Novartis

Johns Hopkins

Roche

Why Choose Us

Up to 266 nt pegRNA synthesis

200+ modification options

Proven >60% editing efficiency

RUO to GMP support

pegRNA Synthesis

110-266 nt, chemically synthesized

PE mRNA

PE2, PE3, PEmax variants

Nicking sgRNA

PE3 system components

Editing Efficiency

>60%

Service Overview

Comprehensive Prime Editing Solutions

Our platform delivers end-to-end Prime Editing support, from chemically synthesized pegRNAs to PE mRNA, enabling precise "search-and-replace" genome editing without double-strand breaks.

pegRNA Synthesis

Our proprietary chemical synthesis platform delivers high-quality pegRNAs up to 266 nucleotides. Default 2'-O-methyl and phosphorothioate modifications enhance stability and editing efficiency in Prime Editing experiments.

Length range: 110-266 nt
Desalt or HPLC purification
200+ modification options

PE mRNA & Components

Comprehensive PE system components including PE2, PE3, and PEmax mRNA variants. Codon-optimized, N1-methylpseudouridine modified for enhanced expression and reduced immunogenicity.

Cap1 structured mRNA
Multiple PE variants available
Validated protocols included

Extended Length

Industry-leading 266 nt synthesis capability for complex pegRNA designs.

Quality Verified

ESI-MS verification and HPLC purity reports for every product.

Flexible Options

From RUO to GMP-grade products to support your research pathway.

Technology Platform

Advanced Prime Editing Technologies

State-of-the-art synthesis and quality control platforms for reliable Prime Editing components.

Chemical Synthesis

Proprietary phosphoramidite chemistry with optimized coupling efficiency for long RNA synthesis.

Up to 99.5% coupling efficiency
110-266 nt length range
Batch-to-batch consistency

Quality Control

Rigorous ESI-MS and HPLC quality verification for every product.

Mass verification
Purity ≥85% (HPLC grade)
Detailed COA reports

Modifications

Extensive modification options to enhance stability and editing efficiency.

2'-O-methyl modifications
Phosphorothioate linkages
200+ custom options

Available PE System Variants

Variant	Description	Application
PE2	Enhanced M-MLV RT with 5 mutations	Standard Prime Editing
PE3	PE2 + nicking sgRNA for non-edited strand	Improved editing efficiency
PEmax	Codon-optimized, additional NLS	Recommended for most applications
PE6	Compact variant (810 bp smaller)	Viral delivery applications

Specifications

Product Specifications

Comprehensive specifications for our Prime Editing components.

pegRNA Specifications

Length Range 110-266 nt

Default Modifications 2'-O-Me, PS

Purification Desalt / HPLC

HPLC Purity ≥85%

Quantity 2-100 nmol

Quality Control ESI-MS + COA

PE mRNA Specifications

Variants PE2, PE3, PEmax

5' Cap Cap1

UTR Optimized

Poly(A) Tail 100 nt

Nucleoside Mod m1Ψ optional

Purity dsRNA-free

Precise Molecular Weight

Mass verification ensures accurate sequence

Batch Consistency

Reproducible results across batches

Low Cytotoxicity

High purity minimizes cellular stress

High Efficiency

Validated >60% editing efficiency

Service Workflow

From Design to Delivery

Streamlined process for your Prime Editing component needs.

Design & Order

Use our online tools to design pegRNAs or specify your PE mRNA requirements

Synthesis

Proprietary chemical synthesis with quality at every step

QC & Verification

ESI-MS mass verification and HPLC purity analysis

Delivery

Secure packaging with comprehensive QC report

Applications

Diverse Applications Across Biotechnology

Our Prime Editing services support research and development in multiple fields.

Gene Therapy Development

Prime Editing enables precise correction of disease-causing mutations without double-strand breaks, offering a safer approach for therapeutic genome editing. The technology supports correction of single nucleotide variants, small insertions, and deletions.

Single nucleotide correction
Sickle cell disease models
β-thalassemia research
Precision medicine development

>90%

Of pathogenic mutations addressable

Cell Therapy Engineering

Engineer CAR-T cells and other immune cells with precision using Prime Editing. Achieve precise knock-ins and corrections without the risks associated with double-strand break-based methods.

CAR-T cell engineering
Hematopoietic stem cells
TCR engineering
Multiplex editing

41%

HSC editing efficiency (literature)

Basic Research Applications

Support your genomics research with high-quality Prime Editing components. Ideal for gene function studies, disease modeling, and pathway analysis.

Gene function studies
Disease model construction
Pathway analysis
Agricultural biotechnology

>60%

Editing efficiency validated

Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The pegRNA quality exceeded our expectations. Sequence accuracy was perfect and the HPLC purity report gave us confidence in our experiments. Highly recommended for Prime Editing research!"

Senior Scientist

Biotechnology Company

"Fast delivery and excellent communication throughout our gene therapy project. The PE mRNA quality was outstanding and the technical support team provided valuable experimental guidance."

Research Director

Pharmaceutical Company

"We've used this service for multiple Prime Editing projects in our lab. Consistent quality and professional service every time. The online design tool is also very helpful."

Lead Researcher

Academic Research Institution

Scientific Literature

Supporting Research

Key publications in Prime Editing technology for your reference.

156 citations

Ex vivo prime editing of patient haematopoietic stem cells rescues sickle-cell disease phenotypes

Everette KA et al. Nature Biomedical Engineering, 2023

View DOI

89 citations

Increasing intracellular dNTP levels improves prime editing efficiency

Liu P et al. Nature Communications, 2024

View DOI

68 citations

Prime Editing: A Revolutionary Technology for Precise Treatment of Genetic Disorders

Zhang L et al. Clinical Pharmacology & Therapeutics, 2024

View DOI

42 citations

Prime editor with rational design and AI-driven optimization for reverse editing window

Chen J et al. Nature Communications, 2025

View DOI

85 citations

Prime editing in mammals: From promise to practicalities

Anzalone AV et al. Molecular Therapy: Nucleic Acids, 2025

View DOI

FAQ

Frequently Asked Questions

Find answers to common questions about our Prime Editing services.

What is the standard length of pegRNA?

Standard pegRNA length ranges from 110-266 nucleotides, depending on the spacer length, PBS length, and RT template. Our synthesis platform supports the full range needed for Prime Editing experiments.

What modifications are recommended for pegRNA?

We recommend including 2'-O-methyl modifications and phosphorothioate linkages at both ends of the pegRNA. These modifications enhance nuclease resistance and improve editing efficiency. Our default modification strategy includes these enhancements.

What purification methods are available?

We offer both desalt and HPLC purification. Desalt purification is suitable for most applications, while HPLC purification (guaranteeing ≥85% purity) is recommended for high-precision experiments or critical applications.

What PE variants do you offer?

We offer PE2, PE3, PEmax, and PE6 mRNA variants. PEmax is our recommended standard for most applications due to its optimized codon usage and enhanced nuclear localization. Contact us for specific variant recommendations.

What is the expected editing efficiency?

Under optimized conditions, our pegRNAs have demonstrated editing efficiencies exceeding 60% in HEK293T cells. Actual efficiency may vary depending on target locus, cell type, and delivery method. We provide validated protocols to help maximize your results.

Do you support GMP-grade products?

Yes, we support projects from RUO (Research Use Only) through INDEdit to cGMP-grade production. This comprehensive pathway supports your transition from research to clinical applications.

What quality reports are included?

Every product includes Certificate of Analysis (COA) and ESI-MS mass verification reports. HPLC-grade products also include HPLC purity reports. All reports are accessible through our online portal.

Request a Quote

Prime Gene Editing Services

Why Choose Us

pegRNA Synthesis

PE mRNA

Nicking sgRNA

Comprehensive Prime Editing Solutions

pegRNA Synthesis

PE mRNA & Components

Extended Length

Quality Verified

Flexible Options

Ready to Start Your Prime Editing Project?

Advanced Prime Editing Technologies

Chemical Synthesis

Quality Control

Modifications

Available PE System Variants

Product Specifications

pegRNA Specifications

PE mRNA Specifications

Precise Molecular Weight

Batch Consistency

Low Cytotoxicity

High Efficiency

From Design to Delivery

Design & Order

Synthesis

QC & Verification

Delivery

Diverse Applications Across Biotechnology

Gene Therapy Development

Cell Therapy Engineering

Basic Research Applications

What Our Clients Say

Supporting Research

Ex vivo prime editing of patient haematopoietic stem cells rescues sickle-cell disease phenotypes

Increasing intracellular dNTP levels improves prime editing efficiency

Prime Editing: A Revolutionary Technology for Precise Treatment of Genetic Disorders

Prime editor with rational design and AI-driven optimization for reverse editing window

Prime editing in mammals: From promise to practicalities

Frequently Asked Questions

Ready to Start Your Project?

Recommended Services

Related Resources