CD Biosynsis is dedicated to improving the productivity of fumaric acid in cellular systems through genetic engineering, metabolic engineering, and enzyme engineering techniques, and to establishing optimal parameters for biosynthesis through precision fermentation techniques to provide ideas for improving microbial production of organic acids.
Fumaric acid is an important platform chemical that can be easily esterified and polymerized due to its multifunctional structure, making it a raw material for industrial products such as resins, unsaturated polyester resins, and alkyd resins. Fumaric acid has been used in the production and processing of food, beverages, detergents, animal feed, pharmaceuticals, and various industrial products. Currently, the petrochemical production route of fumaric acid uses hydrocarbons as raw materials, and carbon monoxide and carbon dioxide are formed during the production process, causing harm to the environment. With the depletion of crude oil, the increasing demand for fumaric acid, and the emphasis on sustainability, synthetic biology-driven fumaric acid generation has become a focus of research.
Figure 1. The metabolic pathway for fumaric acid production. (Xu Q, et al., 2012)
Based on a synthetic biology platform, we are working to improve the fermentation process of fumaric acid using renewable raw materials and genetically modified microorganisms. The following are the platforms and tools involved in providing fumaric acid biosynthesis services. Included but not limited to.
Bacteria, fungi, and yeast can be used as chassis organisms for the production of fumaric acid. Genetic modification of microorganisms is considered to be an important tool to increase the yield of fumaric acid. We are able to provide the following services to help our customers achieve the modification of fumaric acid production strains.
We offer services for UV, radiation, or chemical inducer-driven microbial mutagenesis and help our customers screen for suitable mutant strains.
Fumaric acid is produced in the oxidative pathway of the tricarboxylic acid cycle and accumulated in the reductive pathway of the tricarboxylic acid cycle. Multiple enzymes are involved in the synthesis of fumaric acid from pyruvate, and we are able to help our customers overexpress genes encoding related enzymes to increase the production of fumaric acid.
We are able to help our customers construct recombinant microorganisms for fumaric acid production. We are also able to rapidly analyze the metabolic profile of the modified microorganisms using metabolomics analysis techniques.
The particulate form of the fungus, which relieves the high viscosity of the fermentation broth and supports the recirculation of subsequent fermentation batches, is considered the most favorable form for fumaric acid production. We are able to control the morphology of the fungi by adjusting the medium composition, trace metal ion concentration, spore concentration, pH, temperature, etc.
We are able to optimize the fermentation conditions for fumaric acid production by controlling fermentation parameters such as temperature, metal ion concentration, and dissolved oxygen concentration to improve the fumaric acid productivity of microorganisms.
CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive fumaric acid production and meet the needs of customers in a variety of industries.
CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in fumaric acid production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.