Synthetic Biology
Home / Services / By Technology / DNA and RNA Synthesis Services / Genome Synthesis and Assembly / Adenovirus Genome Synthesis
Trusted by Leading Research & Pharma Institutions

Adenovirus Genome Synthesis Services

Accelerate your adenovirus research with custom genome synthesis leveraging advanced DNA assembly technologies. From full-genome reconstruction to targeted modifications, we deliver infectious clones with industry-leading accuracy and comprehensive quality verification.

Full-Genome Assembly
30kb+ Capacity
Infectious Clone
Learn More

Trusted by leading research and pharmaceutical institutions

Johns Hopkins
Moderna
Stanford
Regeneron
MIT
GSK

Why Choose Us

Isothermal assembly for accurate reconstruction
Full-genome capacity up to 36 kb
Sanger & NGS verification included
Expert consultation on design

Full-Genome Synthesis

Complete adenovirus genome assembly

Custom Modifications

E1/E3 deletions, fiber modifications

Virus Recovery Support

HEK293 packaging cell consultation

Genome Size
36 kb
Overview Technology Specifications Workflow Applications FAQ
Service Overview

Complete Adenovirus Genome Solutions

Our platform combines advanced DNA assembly technologies with comprehensive quality control for accurate adenovirus genome reconstruction.

Full-Genome Synthesis

Complete adenovirus genome reconstruction using advanced isothermal assembly technology. Our platform supports genomes up to 36 kb with high fidelity and accuracy.

  • Wild-type genome reconstruction
  • Multi-fragment assembly (6+ fragments)
  • 30-40 bp overlap design optimization

Custom Modifications

Precise genome engineering including E1/E3 deletions, fiber modifications, and transgene insertion. Modular design enables flexible vector customization.

  • E1/E3 gene deletions
  • Fiber protein modifications
  • Reporter gene insertion

Sequence Verified

Complete genome sequencing verification on every construct.

Infectious Clone

Transfection-ready plasmids for virus recovery in packaging cells.

Expert Support

Consultation on vector design and packaging cell line selection.

Ready to Start Your Project?

Get a customized quote for your adenovirus genome synthesis needs.

Technology Platform

Advanced Assembly Technologies

Industry-leading DNA assembly methods for accurate adenovirus genome reconstruction.

Isothermal Assembly

Efficient one-pot assembly at constant temperature using exonuclease, DNA polymerase, and DNA ligase for seamless fragment joining.

50°C Reaction High Fidelity

Modular Assembly

Modular assembly platform enabling flexible genome engineering in E1/E3 regions with standardized components.

Standardized Parts Flexible

Quality Verification

Comprehensive sequencing verification including Sanger sequencing and next-generation sequencing for long constructs.

Sanger + NGS 100% Accuracy

Quality Control

SEQ Complete genome sequencing
RE Restriction enzyme analysis
QC Clone stability verification
COA Certificate of Analysis included

Deliverables

DNA Plasmid DNA in E. coli glycerol stock
MAP Vector map and sequence files
SEQ Sequencing chromatograms
RPT Comprehensive QC report
Specifications

Service Specifications

Comprehensive specifications for diverse research requirements.

Parameter Specification
Genome Size Up to 36 kb (full adenovirus genome)
Assembly Method Isothermal assembly / Gibson assembly
Fragment Overlap 30-40 bp homologous regions
Vector Type Infectious clone in plasmid backbone
Host Strain E. coli (high-efficiency transformation)
Modifications E1/E3 deletions, fiber modifications, transgene insertion
Quality Verification Sanger sequencing, restriction analysis
Delivery Format Glycerol stock, plasmid DNA, sequence files
Workflow

Streamlined Process from Design to Delivery

Our proven workflow ensures quality and efficiency at every stage.

1

Consultation

Project design and sequence review

2

Design

Fragment design and overlap optimization

3

Assembly

Isothermal assembly and transformation

4

Verification

Sequencing and QC analysis

5

Delivery

Clones and documentation shipped

Applications

Diverse Applications in Research & Development

Our adenovirus genome synthesis services support multiple research and therapeutic development areas.

Gene Therapy Development

Adenovirus vectors are widely used in gene therapy due to their high transduction efficiency and ability to infect both dividing and non-dividing cells. Our genome synthesis enables precise engineering of therapeutic vectors.

  • First/second/third generation vector design
  • Gutless adenovirus for large transgenes
  • Targeted modification for safety
  • Non-integrating delivery system
36 kb
Maximum transgene capacity

Vaccine Development

Adenovirus vectors have emerged as leading platforms for vaccine development, including COVID-19 vaccines. Our synthesis services enable rapid generation of vaccine candidates with precise antigen insertion.

  • Rapid vaccine prototype generation
  • Strong humoral and cellular immunity
  • Multiple serotype options
  • Scalable manufacturing compatibility
100%
Sequence accuracy guaranteed

Oncolytic Virotherapy

Engineered adenoviruses can selectively replicate in and destroy tumor cells while sparing normal tissues. Our genome synthesis enables precise deletion of viral genes for tumor-specific replication.

  • Tumor-selective replication
  • Arming with therapeutic transgenes
  • Fiber modification for tumor targeting
  • Combination therapy support
5+
Targeting modification options
Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The adenovirus genome synthesis service exceeded our expectations. The isothermal assembly was fast and the sequence verification confirmed perfect accuracy. Highly recommended for any gene therapy project."

S
Senior Scientist
Gene Therapy Company

"We needed a gutless adenovirus construct for our vaccine study. The team provided excellent consultation on design and delivered a perfect construct. Will definitely use again for future projects."

J
Research Director
Vaccine Research Institution

"The modular assembly platform made it easy to customize our oncolytic adenovirus. Professional service with comprehensive documentation. Our go-to provider for adenovirus genome engineering."

L
Principal Investigator
Oncology Research Lab
Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research in adenovirus engineering.

15 Citations

Construction of Adenoviral Vectors using DNA Assembly Technology

Zou X, Zhu Y, Li C, et al. Journal of Visualized Experiments (J Vis Exp). 2022.

DNA assembly technology for constructing adenoviral vectors through infection clone generation and reporter gene insertion strategies.

View DOI
8 Citations

Novel and efficient method for the reconstruction of adenoviruses through isothermal assembly

Wen K, Resch MD, Mazboudi R, et al. Frontiers in Medical Technology. 2023.

Isothermal assembly method for rapid adenovirus reconstruction in 4 days with 10-day recombinant virus recovery.

View DOI
85 Citations

Synthetic Biology Approaches for Engineering Next-Generation Adenoviral Gene Therapies

Collins L, Thrasher A, Currie D. ACS Nano. 2021.

Synthetic biology methods for engineering adenovirus vectors with enhanced tropism, safety, and therapeutic cargo capacity.

View DOI
12 Citations

Construction and application of adenoviral vectors

Multiple authors. PMC. 2023.

Comprehensive review of adenovirus vector construction methods including Cre/loxP systems and pAdEasy strategies.

View DOI
25 Citations

AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses

Jang Y, Bunz F. STAR Protocols. 2022.

Modular assembly platform for recombinant adenovirus construction with 3-hour assembly time and 5-day infectious virus production.

View DOI
FAQ

Frequently Asked Questions

Find answers to common questions about our service.

We can synthesize full adenovirus genomes up to 36 kb, which covers all standard serotypes including Ad5 and other commonly used vectors. Our isothermal assembly technology enables efficient reconstruction of large genomic DNA fragments with high accuracy.
We primarily use isothermal assembly (also known as Gibson assembly), which combines exonuclease, DNA polymerase, and DNA ligase in a single reaction at 50°C. This method requires 30-40 bp overlapping regions between fragments and provides highly accurate assembly of multiple DNA fragments.
We offer a wide range of modifications including E1/E3 gene deletions for replication-defective vectors, fiber protein modifications (RGD, Ad5/F35 chimeras) for altered tropism, and transgene insertion in E1 or E3 regions. Gutless adenovirus constructs are also available upon request.
Every clone undergoes comprehensive quality control including complete genome sequencing by Sanger sequencing, restriction enzyme analysis for pattern verification, and clone stability testing. For constructs larger than 5 kb, NGS verification is performed to ensure 100% sequence accuracy.
Deliverables include E. coli glycerol stock of the infectious clone, purified plasmid DNA, vector map files, complete sequencing chromatograms, and a Certificate of Analysis with QC results. Optional services include virus recovery in HEK293 cells and custom documentation.
Yes, expert consultation is included with every project. Our team can assist with sequence optimization, modification strategy selection, packaging cell line recommendations, and experimental design for virus recovery and application development.

Ready to Start Your Project?

Get a customized quote for your Adenovirus Genome Synthesis project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

Recommended Services

· Small Genome Synthesis
· Codon optimization service

Related Resources

· Cost and Efficiency Analysis of CRlSPR-Cas9 Knockout Services in Mice and Cell Lines
· Validating CRISPR Knockouts: Best Practices for qPCR, Sequencing, and Functional Assays
· Step-by-Step Guide to Generating CRISPR Knockout Cell Lines for Research
Quick Links

Home

Products

Services

About Us

Contact Us

Follow Us
CONTACT US

USA

Tel:

Email:

Japan

Tel:

Email:

Korea

Tel:

Email:

Copyright © 2026 CD Biosynsis. All rights reserved.