Custom DNA oligos are short, single-stranded DNA sequences that are synthesized to order. They play a crucial role in various fields of molecular biology research, genetic testing, and biotechnology applications. These oligos are designed with specific sequences to meet the unique requirements of researchers and scientists.
Services Offered
Service Process
FAQs
Service Offered
Custom DNA oligos are versatile tools used in various molecular biology applications, and their quality, sequence accuracy, and purity are critical for the success of downstream experiments. Below is a table outlining the application areas and requirements for custom DNA oligos:
| Application Area |
Description |
Requirements |
| PCR Amplification |
Primer sequences for polymerase chain reaction (PCR). |
Knowledge of target sequence, primer design software, DNA synthesizer, suitable reagents (nucleotides, phosphoramidites). |
| DNA Sequencing |
Sequencing primers for Sanger or next-generation sequencing. |
Design of sequencing primers, DNA synthesizer, high-quality reagents, purification methods, compatibility with sequencing platform. |
| Gene Synthesis |
Construction of synthetic genes for various purposes. |
Accurate sequence design, DNA synthesizer, high-quality reagents, purification methods, sequence verification. |
| Site-Directed Mutagenesis |
Introduction of specific mutations into DNA sequences. |
Design of mutagenic primers, DNA synthesizer, reagents for PCR, sequence verification methods. |
| Probe Hybridization |
Production of probes for molecular detection applications, such as fluorescence in situ hybridization (FISH). |
Design of probe sequence, labeled nucleotides or post-synthesis labeling, purification methods, quality control. |
| Amplicon Sequencing |
Primers for targeted sequencing of specific genomic regions. |
Design of amplicon-specific primers, DNA synthesizer, high-quality reagents, purification methods, compatibility with sequencing platform. |
| Real-Time PCR (qPCR) |
Primers and probes for quantitative PCR applications. |
Design of qPCR primers and probes, DNA synthesizer, high-quality reagents, purification methods, compatibility with qPCR instrumentation. |
| Functional Genomics |
Synthesis of custom oligos for various functional genomics studies. |
Design of oligo sequences, DNA synthesizer, purification methods, validation assays. |
Service Process
- Design Consultation: Our expert team will work closely with you to understand your research needs and design the custom DNA oligos accordingly. We will discuss your project requirements, including specific sequences, modifications, and any other customization options you may require.
- Synthesis and Quality Control: Once the design is finalized, our laboratory will begin the synthesis of the DNA oligos using advanced techniques. Throughout the synthesis process, rigorous quality control measures are implemented to ensure the accuracy and purity of the oligos. We utilize state-of-the-art equipment and follow strict protocols to maintain the highest standards of quality.
- Packaging and Delivery: After synthesis and quality control, the DNA oligos will be carefully packaged to maintain their integrity during transit. We understand the importance of preserving the quality of the oligos, and we take every precaution to ensure their safe delivery. The packaged oligos will be delivered to your specified location, ensuring that they reach you in optimal condition.
We are committed to providing you with high-quality custom DNA oligos that meet your specific research needs. Our experienced team, advanced technology, fast turnaround times, and competitive pricing make us the ideal partner for all your custom DNA oligo requirements. Contact us today to discuss your project and let us help you advance your research!
FAQs
Here are some commonly asked questions about our custom DNA oligos:
Q: What is the turnaround time for custom DNA oligos?
A: The turnaround time for custom DNA oligos can vary depending on the specific requirements and order volume. However, we prioritize timely delivery and strive to provide an estimated timeframe for completion. Please contact our team for more information regarding your specific project.
Q: Can you assist with the design of the DNA oligos?
A: Absolutely! Our team of experts is available to provide design consultation and support throughout the entire process. We will work closely with you to understand your research goals and help you design the DNA oligos that meet your specific requirements.
Q: Are there any storage recommendations for the DNA oligos?
A: To maintain the stability and integrity of the DNA oligos for long-term use, we recommend storing them at -20°C. This temperature ensures that the oligos remain in optimal condition and are ready for use whenever you need them.
Q: What applications can custom DNA oligos be used for?
A: Custom DNA oligos have a wide range of applications in molecular biology research and biotechnology. They can be used for techniques such as PCR (Polymerase Chain Reaction), DNA sequencing, gene expression analysis, molecular cloning, and much more. The versatility of custom DNA oligos makes them essential tools for various research applications.
Q: Can I order DNA oligos with modified bases or labels?
A: Yes, we offer options for modified bases and labels for your custom DNA oligos. We understand that specific modifications or labels may be required for your research project.
Q: What is the recommended purification method for custom DNA oligos?
A: We offer various purification options for custom DNA oligos, including standard desalting, HPLC purification, and PAGE purification. The choice of purification method depends on the specific requirements of your research project. Our experts can guide you in selecting the most suitable purification method for your needs.
Q: Can you provide technical support for troubleshooting experiments involving DNA oligos?
A: Absolutely! Our team of experts is available to provide technical support and troubleshooting assistance for experiments involving DNA oligos. We understand that research experiments can sometimes encounter challenges, and we are here to help you overcome them.
Q: Do you offer bulk discounts for large orders of custom DNA oligos?
A: Yes, we offer bulk discounts for large orders of custom DNA oligos. Please contact our team to discuss your order volume and specific requirements, and we will provide you with a competitive pricing quote.
Q: What quality control measures do you have in place for custom DNA oligos?
A: We have stringent quality control measures in place to ensure the accuracy and purity of our custom DNA oligos. Our quality control includes analytical testing to verify the sequence and length of the oligos, as well as purity assessment to ensure minimal levels of impurities.
Q: Can you provide documentation for the quality control tests performed on the DNA oligos?
A: Yes, we can provide documentation for the quality control tests performed on the DNA oligos upon request. Our goal is to ensure transparency and provide you with the necessary information to validate the quality of our products.
Q: What is the shelf life of the custom DNA oligos?
A: When stored properly at -20°C, our custom DNA oligos have a shelf life of up to 24 months. We recommend following proper storage guidelines to maintain the stability and integrity of the oligos throughout their shelf life.
Q: Can you assist with the design of primers for PCR experiments?
A: Yes, our team can assist you with the design of primers for PCR experiments. We have extensive experience in primer design and can help you optimize the primer sequences for your specific PCR applications.
Q: Do you offer DNA oligos with modified backbones for specific applications?
A: Yes, we offer DNA oligos with modified backbones for specific applications. Modified backbones can enhance stability, nuclease resistance, or other properties based on your research needs. Please discuss your requirements with our team, and we will assist you accordingly.
Q: What is your process for ensuring the confidentiality of customer sequences?
A: we prioritize the confidentiality and privacy of our customers' sequences. We have strict data protection measures in place to ensure that your sequences and research information remain confidential. We adhere to industry-standard security practices and protocols to safeguard your data.
Q: Can you provide a certificate of analysis for the custom DNA oligos?
A: Yes, we can provide a certificate of analysis (CoA) for the custom DNA oligos upon request. The CoA includes detailed information about the synthesis, purification, and quality control results of the oligos.
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