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Protein Modification and Labeling Services

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Protein modification and labeling services offer advanced solutions for altering and tagging proteins to study their function, interactions, localization, and activity. These services provide essential tools for researchers in fields such as biochemistry, molecular biology, pharmacology, and biotechnology. Our protein modification and labeling services encompass a wide range of techniques and applications, ensuring that your modified and labeled proteins meet your specific research and biotechnological needs.

Biotin ligase catalyzed protein modification (M Sunbul, et al.,2009)

Overview Service Process Examples and Solutions Applications Frequently Asked Questions


Protein modification involves the chemical or enzymatic alteration of proteins to change their properties, such as stability, solubility, or activity. Labeling proteins with various tags or probes allows researchers to track, detect, and analyze proteins in complex biological systems. Our services utilize state-of-the-art technologies and methodologies to provide precise and efficient protein modifications and labeling tailored to your requirements.

Service Process

The process of protein modification and labeling involves several critical and interrelated steps:

  1. Project Consultation: Collaborating with researchers to define the specific modification or labeling requirements, including the type of modification, target protein, and intended application.
  2. Protein Expression and Purification: Producing and purifying the target protein in a suitable expression system, ensuring high purity and yield.
  3. Modification and Labeling: Applying chemical, enzymatic, or recombinant techniques to modify or label the protein. This may include conjugation with fluorescent dyes, biotin, enzymes, or other tags.
  4. Purification of Modified/Labeled Proteins: Isolating the modified or labeled protein using techniques such as affinity chromatography, size-exclusion chromatography, or ion exchange chromatography to ensure high purity and specificity.
  5. Characterization and Validation: Assessing the modified or labeled protein's purity, stability, functionality, and labeling efficiency using methods such as SDS-PAGE, Western blotting, mass spectrometry, and activity assays.
  6. Optimization and Scale-Up: Refining the modification or labeling process based on initial results and scaling up production to meet the required quantities for research or commercial use.

For more information about our Protein Modification and Labeling Services or to discuss your specific needs, please contact us. Our team of experts is available to provide guidance and support for your research and biotechnological projects, ensuring you achieve your scientific and industrial goals.

Examples and Solutions

The following table provides an overview of various case studies in protein modification and labeling and the solutions we offer to support your research and biotechnological endeavors:

Case Study Description Solutions We Offer
Fluorescent Protein Labeling Labeling proteins with fluorescent dyes for cellular imaging studies. Custom labeling, optimization of labeling conditions, and validation.
Biotinylation for Pulldown Assays Modifying proteins with biotin tags to facilitate streptavidin-based pulldown assays. Biotin conjugation, protein purification, and functional assays.
Enzyme Activity Enhancement Modifying enzymes to improve their catalytic activity and stability. Chemical modification, activity assays, and stability testing.
Therapeutic Protein PEGylation Conjugating therapeutic proteins with polyethylene glycol (PEG) to enhance their pharmacokinetic properties. PEGylation, purification, and pharmacokinetic analysis.
Protein-Protein Interaction Studies Labeling proteins with tags for co-immunoprecipitation and FRET studies. Tag conjugation, interaction assays, and fluorescence measurements.
Diagnostic Probe Development Developing labeled proteins for use in ELISA and other diagnostic assays. Custom labeling, assay development, and validation.


The applications of protein modification and labeling are diverse and impactful, including:

  • Protein Function Studies: Modifying proteins to study their biological roles, interactions, and mechanisms.
  • Cellular Imaging: Labeling proteins with fluorescent or other tags to visualize their localization and dynamics in live cells.
  • Protein-Protein Interaction Analysis: Tagging proteins to investigate their interactions and complexes within cells.
  • Drug Development: Modifying proteins to enhance their therapeutic properties or to screen for potential drug candidates.
  • Diagnostic Assays: Labeling proteins with probes for use in diagnostic tests and biosensors.
  • Enzyme Engineering: Modifying enzymes to improve their catalytic properties, stability, or substrate specificity.

Frequently Asked Questions

Q: What is protein modification and labeling?

A: Protein modification involves chemically or enzymatically altering proteins to change their properties, such as stability or activity. Protein labeling involves attaching tags or probes to proteins to enable detection, tracking, and analysis in various applications.

Q: How are protein modification and labeling performed?

A: Protein modification and labeling are performed through a series of steps including project consultation, protein expression and purification, modification and labeling, purification of modified/labeled proteins, characterization and validation, and optimization and scale-up. Each step ensures the precise and efficient modification or labeling of proteins.

Q: What are the applications of protein modification and labeling?

A: Applications include protein function studies, cellular imaging, protein-protein interaction analysis, drug development, diagnostic assays, and enzyme engineering. Modified and labeled proteins are essential tools for various research and biotechnological applications.

Q: What are the key steps in the protein modification and labeling process?

A: Key steps include project consultation, protein expression and purification, modification and labeling, purification of modified/labeled proteins, characterization and validation, and optimization and scale-up. These steps ensure the successful production of high-quality modified and labeled proteins.

Q: Why are protein modification and labeling important?

A: Protein modification and labeling are important for advancing research, developing new therapies, creating diagnostic tools, and improving industrial processes. They provide essential tools for studying and manipulating proteins in various biological and biotechnological contexts.

Please note that all services are for research use only. Not intended for any clinical use.

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