Synthetic Biology
Home / Contact Us / Resource / Others / Oligo Synthesis Service / Custom Antisense Oligonucleotides
Trusted by Leading Research & Pharma Institutions

Custom Antisense Oligonucleotides

Accelerate your ASO research and development with our custom antisense oligonucleotide synthesis services. From routine gapmers to complex PMO and LNA-modified sequences, we deliver high-purity therapeutic-grade ASOs with comprehensive quality control to meet your discovery and preclinical needs.

99.5% Purity
ESI-MS Verified
Full Modification Support
Learn More

Trusted by leading research and pharmaceutical institutions

MIT
Biogen
Stanford
Roche
Johns Hopkins
Novartis

Why Choose Us

Complete ASO modification support
GAPMER, SMO, LNA, PMO, PNA
Scale from research to GMP
ESI-MS QC on every batch

Gapmer ASOs

PS backbone, 2'-MOE wings, RNase H active

Splice-Switching

PMO and morpholino derivatives

Conjugated ASOs

GalNAc, CPP, cholesterol conjugates

ASO Market
$2.5B
Overview Technology Specifications Workflow Applications FAQ

Custom Antisense Oligonucleotide Synthesis Services

Advance your RNA-targeted research with precision-engineered antisense oligonucleotides. Our synthesis platform supports the full spectrum of ASO chemistries for therapeutic development and basic research applications.

Gapmer ASO Synthesis

Generation 2 ASOs with PS backbone and 2'-MOE wings for enhanced nuclease resistance and RNase H-mediated mRNA degradation.

  • 5-10-5 and 3-10-3 configurations
  • All-PS or mixed backbone options
  • Fully automated synthesis

Splice-Switching Oligos

Phosphorodiamidate morpholino oligomers (PMO) and derivatives for steric blockade of splicing events.

  • Neutral backbone chemistry
  • exon skipping applications
  • DMD and SMA research

Advanced Modifications

LNA, PNA, 2'-F, cEt and other third-generation modifications for increased affinity and stability.

  • High-affinity gapmers
  • Mixed chemistry designs
  • Custom conjugate synthesis

Ready to Accelerate Your ASO Research?

Get expert consultation on ASO design, modification strategies, and scale-up options for your therapeutic program.

View Specifications

Advanced ASO Synthesis Technology

Our synthesis platform combines automated solid-phase synthesis with comprehensive quality control to deliver therapeutic-grade antisense oligonucleotides.

Solid-Phase Synthesis

Automated phosphoramidite chemistry on controlled pore glass (CPG) supports enables high-throughput synthesis with consistent coupling efficiency >99%.

Comprehensive QC

Every batch undergoes rigorous analytical testing including ESI-MS verification, HPLC purity analysis, and optical density quantification.

Flexible Modification

Support for all major ASO chemistries including PS, 2'-OMe, 2'-MOE, LNA, PMO, PNA, and various conjugate systems.

Supported ASO Chemistries

Backbone
  • Phosphorothioate (PS)
  • Phosphodiester (PO)
  • Phosphorodiamidate (PMO)
  • Peptide Nucleic Acid (PNA)
Sugar
  • 2'-O-Methyl (2'-OMe)
  • 2'-O-Methoxyethyl (2'-MOE)
  • 2'-Fluoro (2'-F)
  • Locked Nucleic Acid (LNA)
Conjugates
  • GalNAc (liver targeting)
  • Cholesterol
  • Cell-penetrating peptides
  • Custom conjugates
Nucleobase
  • 5-Methylcytosine
  • Inosine
  • Base analogs
  • G-clamp

Product Specifications

Comprehensive ASO synthesis options from research-grade to GMP-quality for your specific application needs.

Parameter Research Grade Preclinical Grade GMP Grade
Length 12-50 nucleotides 12-50 nucleotides 12-50 nucleotides
Scale 0.5 mg - 100 mg 100 mg - 10 g 1 g - kg scale
Purity (HPLC) ≥75% ≥85% ≥95%
Coupling Efficiency >98% >99% >99.5%
QC Analysis ESI-MS, HPLC ESI-MS, HPLC, CE Full analytical suite
Documentation COA COA, MS report COA, CMC, batch records

Quality Control

  • ESI-MS or MALDI-TOF mass verification
  • IP-RP-HPLC purity analysis
  • Ion-exchange HPLC (where applicable)
  • OD260 quantification

Delivery Format

  • Lyophilized powder (standard)
  • Tube or plate format available
  • Dry ice shipping
  • Custom packaging options

Regulatory Support

  • FDA, EMA compliance documentation
  • ICH Q7 guidelines
  • DMF availability
  • Technical transfer support

Our Service Process

A streamlined workflow designed to accelerate your ASO research from design to delivery.

1

Sequence Design

Submit your target sequence and modification requirements. Our team provides expert consultation.

2

Synthesis

Automated solid-phase synthesis with continuous monitoring ensures consistent, high-quality output.

3

Purification

HPLC purification tailored to your purity requirements. Multiple methods available.

4

QC Testing

Comprehensive analytical testing including mass spectrometry and purity verification.

5

Delivery

Quality-certified products shipped with full documentation package.

Applications

ASO Applications Across Therapeutics & Research

Our antisense oligonucleotide synthesis services support development in multiple therapeutic and research areas.

Therapeutic Oligonucleotide Development

ASOs with advanced chemical modifications for enhanced stability, delivery, and therapeutic efficacy. From early discovery to clinical development.

  • Gapmer ASOs with PS backbone and 2'-MOE wings
  • PMO-based splice-switching oligomers
  • GalNAc-conjugated liver-targeting ASOs
  • Custom conjugate synthesis for enhanced delivery
$2.5B
ASO Therapeutic Market

Neurological Disease Applications

Specialized ASO chemistries designed for CNS delivery and neuronal targeting. Addressing unmet needs in rare and prevalent neurological disorders.

  • Spinal muscular atrophy (SMA) research
  • ALS and Huntington's disease programs
  • Intrathecal delivery formulation support
  • Blood-brain barrier crossing strategies
6+
FDA-Approved CNS ASOs

Basic Research Applications

High-quality ASOs for gene expression studies, target validation, and mechanism investigation. Reliable tools for advancing your research.

  • mRNA knockdown and splicing modulation
  • Drug target identification and validation
  • RNase H and spliceosome mechanism studies
  • Cellular uptake and delivery optimization
99.5%
Synthesis Purity

What Our Partners Say

Trusted by leading pharmaceutical companies and research institutions worldwide.

"The quality and consistency of their ASO synthesis has significantly accelerated our preclinical pipeline. Their modification expertise is unmatched."

Research Director

Biotechnology Company

"Their GMP-grade ASO production capabilities have been essential for our clinical trial material needs. Reliable quality and timely delivery."

Principal Scientist

Pharmaceutical Company

"Excellent technical support and flexibility in handling custom modification requests. They've become our go-to partner for ASO synthesis."

Senior Scientist

Academic Research Institution

Scientific Literature

Key publications supporting ASO technology and therapeutic applications.

156 Citations

Progress in the Use of Antisense Oligonucleotides for Vaccine Improvement

Batista-Duharte A, Sendra L, Herrero MJ, et al. (2020) Biomolecules

Comprehensive review of ASO applications in vaccine development, covering molecular mechanisms, chemical modifications, and therapeutic potential.

View DOI
316 Citations

Recent progress in non-native nucleic acid modifications

McKenzie LK, El-Khoury R, Thorpe JD, et al. (2021) Chemical Society Reviews

Authoritative review of non-native nucleic acid modifications for therapeutic oligonucleotides, including ASO and siRNA chemistries.

View DOI
109 Citations

Chemical synthesis and biological application of modified oligonucleotides

Glazier DA, Liao J, Roberts BL, Li X, Yang K, et al. (2020) Bioconjugate Chemistry

Review of RNA chemical synthesis methods and modified versions, covering PS, 2'-F, 2'-OMe, 2'-MOE, LNA, and PMO chemistries.

View DOI
38 Citations

Drug discovery perspectives of antisense oligonucleotides

Kim Y (2023) Biomolecules & Therapeutics

Recent advances in ASO drug discovery covering chemical modification strategies, pharmacokinetics, and clinical applications.

View DOI
45 Citations

Antisense oligonucleotides: A novel frontier in pharmacological strategy

Collotta D, et al. (2023) Frontiers in Pharmacology

Frontiers article discussing ASO mechanisms, delivery strategies, and clinical translation challenges in modern pharmacology.

View DOI

Frequently Asked Questions

Find answers to common questions about our ASO synthesis services.

What chemical modifications do you support for ASO synthesis?
We support all major ASO chemistries including phosphorothioate (PS) backbone, 2'-O-methyl (2'-OMe), 2'-O-methoxyethyl (2'-MOE), 2'-fluoro (2'-F), locked nucleic acid (LNA), constrained ethyl (cEt), phosphorodiamidate morpholino (PMO), and peptide nucleic acid (PNA). We also offer various conjugate options including GalNAc for liver targeting, cholesterol, and cell-penetrating peptides.
What purity levels can you achieve for ASOs?
We offer multiple purity grades: research grade (≥75%), preclinical grade (≥85%), and GMP grade (≥95%). For most research applications, 75-85% purity is sufficient. For preclinical and IND-enabling studies, we recommend ≥85% purity with full analytical characterization.
What quality control methods are performed on ASO products?
Every batch undergoes comprehensive QC testing including ESI-MS or MALDI-TOF mass verification to confirm identity, IP-RP-HPLC or IEX-HPLC for purity analysis, UV quantification (OD260) for concentration, and solubility testing. GMP-grade products include additional characterization per regulatory requirements.
Can you synthesize modified ASOs like gapmers and PMOs?
Yes, we specialize in all ASO formats including gapmer ASOs with mixed PS/2'-MOE chemistry for RNase H activity, splice-switching PMO oligomers for exon skipping applications, mixed chemistry designs combining different modifications, and conjugated ASOs with GalNAc, peptides, or other targeting moieties.
What scale options are available for ASO synthesis?
We offer flexible scale options from milligrams to kilograms: research scale (0.5 mg - 100 mg) for initial screening and feasibility, preclinical scale (100 mg - 10 g) for animal studies, and GMP scale (1 g - kg level) for clinical trial material. Scale-up capabilities are available for late-stage development and commercial supply.
Do you provide regulatory documentation for ASO products?
Yes, GMP-grade products include comprehensive regulatory documentation: Certificate of Analysis (COA), CMC documentation, batch records, stability data, and DMF availability for IND submissions. We comply with FDA, EMA, and ICH Q7 guidelines. Our team can also support technical transfer and regulatory consultations.

Ready to Start Your Project?

Get a customized quote for your Custom Antisense Oligonucleotides project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

Recommended Services

· Custom Oligo Libraries
· Custom CpG ODNs Synthesis
· Diagnostic Probes Oligos Synthesis
· Custom Modified Oligos
· NGS Oligos Synthesis
· Custom DNA oligos

Related Resources

· Cost and Efficiency Analysis of CRlSPR-Cas9 Knockout Services in Mice and Cell Lines
· Validating CRISPR Knockouts: Best Practices for qPCR, Sequencing, and Functional Assays
· Step-by-Step Guide to Generating CRISPR Knockout Cell Lines for Research
Quick Links

Home

Products

Services

About Us

Contact Us

Follow Us
CONTACT US

USA

Tel:

Email:

Japan

Tel:

Email:

Korea

Tel:

Email:

Copyright © 2026 CD Biosynsis. All rights reserved.