Trusted by Leading Research & Pharma Institutions

Synthetic Biology Chassis Development

Engineered microbial platforms for industrial bioproduction. From genome reduction to pathway optimization, our comprehensive chassis development solutions accelerate your path from concept to commercial-scale manufacturing.

8 Host Species

Genome Reduction

AI-Assisted Design

Learn More

Trusted by leading research and pharmaceutical institutions

Harvard

Pfizer

MIT

Roche

Stanford

Merck

Why Choose Us

50+ validated chassis platforms

WGS verification on every strain

85-90% editing efficiency

Scale-up support included

Proven Platform Strains

E. coli, Bacillus, Pseudomonas, yeast platforms

Custom Engineering

CRISPR editing, genome reduction, pathway optimization

Scale-Up Support

Shake flask to bioreactor process development

Titer Improvement

10x

Service Overview

Industry-Leading Microbial Chassis Platforms

We provide comprehensive synthetic biology chassis development services, offering validated microbial platforms alongside custom engineering solutions for industrial biotechnology applications.

Proven Platform Strains

Access our catalog of validated chassis organisms including E. coli K-12 and E. coli B derivatives, Bacillus subtilis, Pseudomonas putida, and yeast platforms. Each strain comes with comprehensive characterization data and proven track records in industrial applications.

Full genome annotation and WGS verification
Phenotypic profiling and growth characterization
Cryopreserved stocks with COA documentation

Custom Engineering

Our team delivers tailored chassis engineering solutions including genome reduction, pathway optimization, and regulatory element engineering. We leverage both traditional metabolic engineering and cutting-edge AI-assisted design tools.

CRISPR-based genome editing (85-90% efficiency)
Multi-omics integration and metabolic modeling
Machine learning models for design optimization

Rapid Development

From design to characterized strain in as few as 8 weeks for optimized projects.

Quality Guaranteed

Every strain undergoes WGS authentication, phenotype verification, and purity testing.

Scale-Up Support

Process development from shake flask to bioreactor with tech transfer packages.

Complete Documentation

COA, WGS data, genotype reports, and stability studies included.

Technology Platform

Advanced Chassis Engineering Platforms

Our integrated technology stack combines proven molecular biology methods with state-of-the-art computational tools to deliver chassis strains optimized for your specific production requirements.

Genome Reduction

Systematic identification and removal of non-essential genomic regions to create streamlined chassis with improved genetic stability and metabolic efficiency. Our platform achieves 15-20% genome reduction while maintaining parental growth characteristics.

Comparative Genomics Lambda Red Cre-lox

Metabolic Pathway Engineering

Computational design and experimental validation of heterologous pathways integrated into chassis genomes. We employ dynamic regulatory systems for optimal carbon flux distribution across growth and production phases.

Genome-scale Modeling Dynamic Flux

Precision Genome Editing

Advanced CRISPR-Cas systems enable multiplexed genome modifications with high efficiency. Our optimized protocols achieve 85-90% editing efficiency across diverse bacterial and yeast species.

CRISPR-Cas9 Base Editing Multiplex

AI-Assisted Design

Integration of machine learning models for promoter strength prediction, RBS optimization, and pathway design. Our computational pipeline reduces experimental iterations by up to 60% compared to traditional approaches.

Deep Learning DBTL Cycles

High Efficiency

85-90% editing efficiency across diverse hosts

Rapid Development

From design to characterized strain in 8 weeks

Proven Results

Titers improved up to 10-fold vs wild-type

Specifications

Platform Strain Catalog

Choose from our validated chassis platforms or discuss custom engineering options for your specific application requirements.

Host Organism	Strain Type	Primary Applications	Documentation
E. coli K-12 MG1655 derivative	Wild-type / Reduced Genome	Cloning, plasmid production, enzyme expression	Complete
E. coli B BL21(DE3) derivative	Protein Expression	Recombinant protein, metabolic engineering	Complete
Bacillus subtilis 168 derivative	Secretion Host	Secreted proteins, enzymes, spores	Complete
Pseudomonas putida KT2440 derivative	Industrial Chassis	Chemical production, specialty compounds	Complete
Saccharomyces cerevisiae Baker's yeast	Eukaryotic Platform	Protein processing, glycosylation	Complete
Pichia pastoris Komagataella phaffii	Secretion/Eukaryotic	Complex proteins, antibodies	Complete

Workflow

Streamlined Process from Design to Delivery

Our proven 5-step workflow ensures quality and efficiency at every stage.

Consultation

Project scope and timeline agreement

Construction

Strain engineering and pathway integration

Screening

Performance ranking and selection

Characterization

WGS verification and stability studies

Delivery

Strain banking and tech transfer

Standard Timeline

12-18 weeks for custom development

Communication

Bi-weekly updates with direct scientist access

Revision Policy

Two rounds of design modifications included

Applications

Diverse Applications Across Biotechnology

Our chassis platforms support diverse industrial biotechnology applications from small-molecule synthesis to complex protein production.

Metabolic Engineering for Small Molecules

Engineered chassis strains enable efficient production of amino acids, organic acids, alcohols, and other value-added chemicals through optimized metabolic pathways and precursor supply networks.

Amino acids (L-lysine, L-glutamate, L-threonine)
Organic acids (succinic acid, lactic acid, adipic acid)
Alcohols and platform chemicals
Vitamins and cofactors

10x

Titer improvement vs wild-type

Recombinant Protein Production

From industrial enzymes to therapeutic proteins, our optimized expression platforms deliver high yields with proper folding and post-translational modifications for diverse applications.

Industrial enzymes (lipases, proteases, cellulases)
Therapeutic proteins and antibodies
Fusion proteins and conjugates
Membrane proteins and complexes

99.8%

Genetic stability over 50 passages

Specialty Compound Biosynthesis

Engineered chassis enable production of complex natural products, pharmaceutical intermediates, and specialty chemicals through heterologous pathway reconstruction and optimization.

Polyketides and nonribosomal peptides
Terpenoids and sterols
Flavonoids and phenolics
Alkaloids and amino acid derivatives

8 weeks

Rapid development timeline

Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The chassis strain delivered exceeded our productivity targets by three-fold while maintaining excellent genetic stability through multiple fermentation cycles. Their technical team provided exceptional support throughout the project."

Senior Scientist

Biotechnology Company

"Working with this team transformed our metabolic engineering workflow. The genome-reduced strain they developed has become our standard platform for small molecule production, and the documentation quality has streamlined our regulatory submissions."

Research Director

Pharmaceutical Company

"Their expertise in yeast platform engineering helped us achieve glycosylation patterns critical for our therapeutic protein program. The collaboration was highly professional and results-oriented."

Principal Investigator

Academic Research Institution

Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

187 Citations

Engineering and modification of microbial chassis for systems and synthetic biology

Chi H, Wang X, Shao Y, Qin Y, Deng Z, Wang L, Chen S. Synthetic and Systems Biotechnology. 2019.

Comprehensive review of strategies for constructing and modifying microbial chassis organisms for industrial biotechnology applications.

View DOI

49 Citations

Model-guided dynamic control of essential metabolic nodes boosts acetyl-coenzyme A-dependent bioproduction in rewired Pseudomonas putida

Kozaeva E, Volkova S, Matos MRA, Mezzina MP, Wulff T, Volke DC, Nielsen LK, Nikel PI. Metabolic Engineering. 2021.

Demonstrates metabolic model-guided optimization of Pseudomonas putida for enhanced acetyl-CoA availability and improved biopolymer production.

View DOI

156 Citations

New opportunities for genetic code expansion in synthetic yeast

Sanders JEJ, Hoffmann SA, Moody ER, McCullough M, Green AP, Cai Y. Current Opinion in Biotechnology. 2022.

Reviews the Sc2.0 synthetic yeast platform for genetic code expansion and non-canonical amino acid incorporation in eukaryotic systems.

View DOI

78 Citations

De Novo Production of Hydroxytyrosol by Saccharomyces cerevisiae–Escherichia coli Coculture Engineering

Liu Y, Song D, Hu H, et al. ACS Synthetic Biology. 2022.

Demonstrates microbial coculture engineering for efficient production of high-value phenolic compounds through division of labor.

View DOI

95 Citations

A programmable protein expression platform (SynPic-X) in Pichia pastoris based on CRISPR-Cas and signal amplifier modules

Liu Q, Cai M, et al. Science Advances. 2022.

Development of CRISPR-Cas based programmable expression system for Pichia pastoris achieving high-level, tunable protein production.

View DOI

FAQ

Frequently Asked Questions

Find answers to common questions about our service.

What chassis organisms do you offer?

We offer validated platforms across eight major host organisms: E. coli K-12 and B derivatives, Bacillus subtilis, Pseudomonas putida, Corynebacterium glutamicum, Saccharomyces cerevisiae, Pichia pastoris, and Yarrowia lipolytica. Each comes with comprehensive characterization data and documentation packages.

What is the typical timeline for custom chassis development?

Standard custom chassis development projects require 12-18 weeks from project kick-off to final strain delivery. Timeline depends on project complexity, number of modifications, and screening requirements. Expedited timelines may be available for select projects.

What level of genome modification is possible?

Our capabilities span from single gene knockouts to multi-site modifications including genome reduction (up to 15% genome removal), pathway integration (up to 15 genes), regulatory element engineering, and chromosomal rearrangements. Complex projects are scoped during consultation.

What documentation is provided with strains?

Every strain delivery includes: Certificate of Analysis (COA), whole-genome sequencing data, genotype verification report, passage stability data, growth characterization curves, handling and storage protocols, and bioinformatics annotation files. Regulatory documentation packages available upon request.

Can you support scale-up to manufacturing?

Yes, we provide integrated scale-up support including shake flask to bioreactor process development, fermentation optimization, and comprehensive tech transfer packages for manufacturing teams. Our technical consultants remain available throughout your scale-up journey.

What quality control standards are followed?

All strains undergo rigorous QC including: whole-genome sequencing authentication, mycoplasma testing, sterility testing, viability verification, and phenotypic characterization. Our quality management system follows ISO standards and supports regulatory compliance for pharmaceutical and food applications.

What is the turnaround time for catalog strains?

Catalog strains are typically available within 3-5 business days for standard formats. Cryopreserved glycerol stocks, multiple aliquots, and custom documentation packages may extend lead time. Express shipping options are available for urgent requirements.

Do you offer strain authentication services?

Yes, we provide comprehensive strain authentication services including whole-genome sequencing, SNP profiling, PFGE analysis, and phenotype verification. These services are available as standalone offerings or as part of our strain development programs.

Ready to Start Your Chassis Development Project?

Contact our technical team to discuss your requirements, timeline, and receive a customized quote for your synthetic biology chassis development needs.

Ready to Start Your Project?

Get a customized quote for your Synthetic Biology Chassis Development project. Our experts will respond within 24 hours.

No obligation

24h response

Expert consultation

Request a Quote

Synthetic Biology Chassis Development

Why Choose Us

Proven Platform Strains

Custom Engineering

Scale-Up Support

Industry-Leading Microbial Chassis Platforms

Proven Platform Strains

Custom Engineering

Rapid Development

Quality Guaranteed

Scale-Up Support

Complete Documentation

Ready to Build Your Production Platform?

Advanced Chassis Engineering Platforms

Genome Reduction

Metabolic Pathway Engineering

Precision Genome Editing

AI-Assisted Design

Platform Strain Catalog

Streamlined Process from Design to Delivery

Consultation

Construction

Screening

Characterization

Delivery

Diverse Applications Across Biotechnology

Metabolic Engineering for Small Molecules

Recombinant Protein Production

Specialty Compound Biosynthesis

What Our Clients Say

Scientific Foundation

Engineering and modification of microbial chassis for systems and synthetic biology

Model-guided dynamic control of essential metabolic nodes boosts acetyl-coenzyme A-dependent bioproduction in rewired Pseudomonas putida

New opportunities for genetic code expansion in synthetic yeast

De Novo Production of Hydroxytyrosol by Saccharomyces cerevisiae–Escherichia coli Coculture Engineering

A programmable protein expression platform (SynPic-X) in Pichia pastoris based on CRISPR-Cas and signal amplifier modules

Frequently Asked Questions

Ready to Start Your Chassis Development Project?

Ready to Start Your Project?

Recommended Services

Related Resources