Synthetic biology Chassis Development

Engineered microbial and Mammalian Hosts for High-Performance Production

Transform standard host strains into optimized chassis for efficient biosynthesis of biologics, materials, and specialty chemicals.

Custom metabolic pathway engineering
CRISPR-based precision genome editing
Targeted strain screening and characterization

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Glyco-Engineered Chassis

Specialized development of glycofactories with optimized sugar donor pools and glycosyltransferase expression for high-fidelity product output.

Concurrent knockout and heterologous pathway integration
Metabolic flux analysis to identify bottlenecks
Humanization of yeast or bacterial strains

Microbial Hosts

Mammalian Hosts

Custom Pathways

Our Approach

Rational Chassis Development Through Integrated Synthetic biology

We combine precision genome editing with metabolic pathway reconstruction to create robust production strains tailored to your target product.

Precision Genome Editing

Our CRISPR-based platform enables concurrent modifications of multiple genetic loci, including targeted knockouts and site-specific integration of heterologous biosynthetic pathways.

Multi-locus knockouts of endogenous proteases or glycosidases
Site-specific integration of heterologous glyco-pathways
Whole-genome sequencing for off-target detection

Metabolic Pathway Reconstruction

Using dynamic flux analysis and rational design, we redirect cellular resources toward the target metabolite by eliminating competing pathways and optimizing precursor supply.

Flux balance modeling of carbon and energy networks
Redirection of metabolic resources toward target biosynthesis
Elimination of competing metabolite sinks

Host Selection

Choose the most suitable microbial or mammalian host based on desired post-translational modifications and application requirements.

Design-Build-Test Cycle

Iterative rounds of genetic design, construction, and phenotypic screening to converge on a high-performance chassis.

Comprehensive QC

Validation at genotype and phenotype levels, including growth kinetics, product titer, and off-target assessment.

Project Deliverables

What You Receive at Project Completion

Each project delivers a fully characterized engineered strain along with a comprehensive data report.

Physical Deliverables

Engineered chassis strain (bacterial or yeast glycerol stock)
Sequence-verified plasmids and genetic constructs
Parental control strain for comparison
Detailed strain documentation

Data Deliverables

Genome editing confirmation report (Sanger or NGS)
Growth kinetics and metabolite titer data
Metabolic flux analysis summary (if scoped)
QC results including off-target assessment

Service Scope

Customizable Project Parameters

Each project is scoped individually based on your target product and host requirements. The table below outlines typical parameters.

Parameter	Typical project scope
Host organism	E. coli, S. cerevisiae, P. pastoris, or other microbial/mammalian cells; custom host available upon request
Number of genetic modifications	As scoped per project; typical range 1–5 targeted loci
Editing type	Knockout is primary; knock-in and precise point mutations available case-by-case
Integration method	CRISPR-Cas9 or variant; site-specific recombinase when applicable
Strain screening approach	Colony PCR, restriction digest, or sequencing for genotype; growth curve and product titer for phenotype
Off-target analysis	In silico prediction plus targeted amplicon sequencing; whole-genome sequencing when scoped
Metabolic analysis	Targeted metabolite profiling or flux analysis based on project needs
Project deliverable format	Glycerol stock, sequenced plasmid, QC report

Workflow

How We Engineer Your Chassis

A streamlined five-step process from initial discussion to final strain delivery.

Host Selection and Pathway Design

We work with you to select the optimal host and design the metabolic modifications needed to achieve your target product. This includes identifying pathways to knock out or introduce.

Genetic Tool Construction

Our team builds CRISPR vectors, repair templates, and any necessary donor constructs for the planned edits. All constructs are sequence-verified before use.

Genome Editing and Strain Generation

Transformation of the host with editing tools, followed by isolation of edited clones. We perform multi-locus editing when required, using optimized protocols for efficient modification.

Strain Screening and Characterization

Clones are genotyped by PCR/sequencing and phenotyped for growth kinetics and product titer. Metabolic flux analysis is applied if scoped to confirm desired pathway redirection.

Data Analysis and Report Delivery

We compile all experimental data, including QC results, into a project report including methods, QC, and datasets. The engineered strain and associated materials are delivered along with the documentation.

Applications

Where Engineered Chassis Make an Impact

Our chassis development service supports a wide range of industries requiring stable, high-yield production strains.

Therapeutic Protein Production

Engineer microbial hosts to produce complex biologics such as glycosylated antibodies, cytokines, and enzymes with improved fidelity and yield.

Humanized glycosylation patterns
Immunogenicity risk assessed analytically case by case
Higher product consistency

Specialty Chemical Synthesis

Evaluate metabolic pathways for the production of fine chemicals, amino acids, vitamins, and other high-value compounds.

Increased titer and yield
Reduced byproduct formation
Improved substrate utilization

Bio-based Polymers and Materials

Develop chassis strains that convert renewable feedstocks into biodegradable polymers, building blocks for plastics.

Renewable feedstocks
Reduced environmental footprint
Scalable production

FAQ

Common Questions About Chassis Development

Answers to typical concerns when planning a strain engineering project.

Each project includes host selection, design of genetic modifications, construction of editing tools, generation of engineered strains, screening and characterization (growth kinetics, product titer), and a final report with QC data. Physical deliverables include glycerol stocks, plasmids, and control strains.
We perform in silico off-target prediction using genome alignment tools, followed by targeted amplicon sequencing of predicted off-target sites. Whole-genome sequencing can be added if scoped to provide a comprehensive off-target profile.
Yes, we can accept customer-provided host strains subject to confidentiality and material transfer agreements. The final engineered strain and all generated materials remain your exclusive property.
Project duration depends on the number of targeted modifications, screening depth, and host organism. A timeline will be defined in the project proposal after consultation and is typically scoped to meet your deadline.

Scientific Literature

References

Yang X, Ye M, Wang F, et al. A Nitrate/Nitrite Biosensor Designed with an Antiterminator for In Vivo Diagnosis of Colitis Based on Bacteroides thetaiotaomicron. ACS synthetic biology. 2025;14(2):453-462. View on PubMed
Seco EM, Fernández LÁ. Efficient markerless integration of genes in the chromosome of probiotic E. coli by bacterial conjugation. Microbial biotechnology. 2022;15(5):1374-1391. View on PubMed
Zheng C, Li Y, Wu X, et al. Advances in the Synthesis and Physiological Metabolic Regulation of Nicotinamide Mononucleotide. Nutrients. 2024;16(14). View on PubMed
Kim K, Choe D, Lee DH, et al. Engineering Biology to Construct Microbial Chassis for the Production of Difficult-to-Express Proteins. International journal of molecular sciences. 2020;21(3). View on PubMed
Brown EA, Brevi A, Zong DM, et al. Engineering commensal microbes for host health. Cell host & microbe. 2026;34(7):1241-1261. View on PubMed

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