Gene Synthesis Service

Gene Synthesis is the chemical and enzymatic manufacturing of a complete, double-stranded, sequence-verified gene, typically cloned into a standard or custom plasmid vector. This service is essential for researchers needing ready-to-use, high-quality genetic material for protein expression, functional genomics, metabolic pathway construction, and vaccine development. It is the most reliable method for obtaining complex or long DNA sequences that are difficult or impossible to procure through traditional cloning or PCR.

CD Biosynsis’s Gene Synthesis platform is engineered for speed, accuracy, and complexity . We specialize in synthesizing challenging sequences—including those with high GC content, extensive repeats, or secondary structures—and provide proprietary Codon Optimization to maximize the expression of your target protein in any host organism. Every synthesized gene is guaranteed to be 100% sequence-accurate, saving you valuable time and resources in the critical "Build" phase of your synthetic biology projects.

Highlights

Key advantages of choosing our Gene Synthesis service for your projects:

• Guaranteed 100% Accuracy: Full-length sequencing verification (Sanger and/or NGS) ensures every base in the cloned gene is correct, eliminating costly downstream errors.
• Advanced Codon Optimization: Free, AI-driven optimization algorithms dramatically enhance target protein expression and solubility across diverse hosts (E. coli, Mammalian, Yeast, Insect, Plant).
• Complexity Mastered: Successfully synthesize long genes (up to 20 kb+) and those with challenging features such as homopolymers, toxic sequences, or high/low GC content.
• Flexible Delivery: Genes are delivered fully cloned into your choice of standard or custom vector, ready for immediate use in transfection, expression, or downstream assembly.

Applications

Our synthesized genes power critical research and development efforts across the biotech spectrum:

Recombinant Protein Production

           

High-yield synthesis of genes for industrial enzymes, therapeutic antibodies, and complex protein targets using optimized expression systems.

Synthetic & Metabolic Pathway Construction

Accurate, high-fidelity synthesis of multiple genes simultaneously, facilitating the rapid construction of entire novel metabolic or regulatory pathways.

Functional Genomics Studies

Creation of synthetic gene libraries, truncated genes, or fusion constructs for high-throughput screening, target validation, and mutagenesis campaigns.

Vaccine & Therapeutic Development

Fast, high-quality synthesis of viral antigens, therapeutic proteins, or DNA/RNA vaccine candidates for preclinical studies.

Solutions

We provide comprehensive solutions to ensure seamless integration into your research pipeline.

Advanced Codon Optimization (OptiGen™)

Algorithm-driven optimization of mRNA stability, ribosome binding efficiency, and protein folding to maximize expression in your host system.

Complex Sequence Assembly

Utilization of proprietary error-correction and multi-fragment assembly methods to efficiently build sequences up to 20 kb and beyond with high accuracy.

Free Subcloning & Vector Selection

Cloning of the synthesized gene into your preferred destination vector (over 100 common vectors available) or into your custom-provided plasmid at no additional cost.

High-Quality Plasmid Prep

Delivery of microgram quantities of high-purity, endotoxin-free plasmid DNA, suitable for sensitive cell transfection and in vivo studies.

Large DNA Fragment & Pathway Synthesis

Specialized service for synthesizing multiple genes simultaneously for large-scale projects, such as pathway engineering or whole-genome modifications.

Workflow

Our validated Gene Synthesis workflow ensures accuracy and timely delivery of your critical research material:

• Sequence Review & Codon Optimization: Upon submission, we analyze the sequence complexity and perform our complimentary OptiGen™ codon optimization to maximize protein expression in your specified host.
• Oligo Synthesis & Assembly: The full gene sequence is broken into small oligonucleotide components. These oligos are chemically synthesized and then assembled enzymatically and/or via PCR-based methods into the full-length gene fragment.
• Cloning & Quality Control: The assembled gene is cloned into the client's vector of choice. Presumptive clones are screened and then subjected to full double-strand sequencing (Sanger/NGS) for 100% sequence verification.
• Plasmid Preparation & Final QC: Verified positive clones are expanded, and the high-quality plasmid DNA is purified. Final QC checks for concentration, integrity, and (if requested) endotoxin levels.
• Delivery & Documentation: The sequence-verified plasmid DNA is shipped with a comprehensive data package, including the Certificate of Analysis (COA), full sequencing chromatograms, final sequence file, and vector map.

We are dedicated to providing the highest level of product quality and technical support:

• Zero Error Guarantee: We guarantee 100% sequence accuracy for all cloned genes; if an error is found, we resynthesize the gene at no cost.
• Free Technical Consultation: Access to our molecular biology experts for advice on codon optimization, vector selection, and downstream application strategies.
• Intellectual Property Protection: Strict confidentiality protocols and secure data handling maintain the proprietary nature of your submitted sequences.
• Reliable Timelines: Clear project timelines are provided upfront, ensuring your project stays on schedule for the downstream "Build" and "Test" phases.