Epigenome Editing Services

It depends on the modification type. Histone modifications are often transient, while DNA methylation via dCas9-DNMT can lead to stable, heritable gene silencing across cell divisions. DNA demethylation using dCas9-TET1 can permanently remove methylation marks once established.

Absolutely. One of the greatest strengths of epigenome editing is targeting distal enhancers and insulators to study the "dark matter" of the genome and its role in long-range gene regulation. This capability is essential for understanding complex regulatory networks.

We use specialized assays including Methylation-Specific PCR, Bisulfite Sequencing, and ChIP-qPCR to verify the addition or removal of chemical marks at the target site. RNA-seq can confirm downstream transcriptional changes.

Yes. Since epigenome editing does not rely on HDR, it is highly effective in post-mitotic cells such as primary neurons, cardiomyocytes, and resting immune cells. This makes it ideal for studying differentiated cells and hard-to-transfect primary tissues.

We offer multiple delivery options: RNP complexes for minimal toxicity, lentiviral vectors for stable integration, AAV for in vivo applications, and mRNA for transient expression. The optimal method depends on your cell type and experimental goals.

DNA methylation editors typically affect 300-500 bp regions, while demethylation editors (TET1) work within 150-200 bp downstream of the PAM site. Histone editors provide target-specific modifications without defined ranges. These can be tailored based on your experimental requirements.