Trusted by Leading Research & Pharma Institutions

Cell-Free Antibody Production Services

Accelerate your antibody discovery with our automated cell-free protein synthesis platform. From gene sequence to functional antibody data in less than 24 hours—bypassing cell toxicity and cultivation bottlenecks.

24hr Turnaround

No Cell Toxicity

High Throughput

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Trusted by leading research and pharmaceutical institutions

Harvard

Pfizer

MIT

Roche

Stanford

Merck

Why Choose Us

Gene to functional data in 24 hours

Express toxic proteins

Automated high-throughput platform

Scalable from screening to production

Rapid Screening

10,000+ variants per campaign

Mammalian CFPS

Proper folding and modifications

No Toxicity

Express difficult proteins

Assembly

99%+

Service Overview

Why Cell-Free Antibody Production?

Traditional cell-based antibody production faces long cultivation cycles, cell toxicity issues, and complex scaling challenges. Our cell-free protein synthesis (CFPS) platform eliminates these bottlenecks.

Rapid Speed

Move from digital gene sequences to functional antibody data in less than 24 hours. No transfection, no cell culture, no waiting weeks for results. Our streamlined workflow compresses months of traditional development into days.

24-hour turnaround for screening
Automated high-throughput workflow
Rapid variant screening

No Cell Toxicity

Express difficult or toxic proteins that would typically compromise a living host cell. Our open reaction system handles what cells cannot, including membrane proteins, regulatory kinases, and antimicrobial peptides.

Express toxic proteins
Membrane proteins supported
No host cell limitations

Batch Consistency

Automated environments remove biological variability.

Cold-Chain Independent

Field-ready systems for remote deployment.

High Throughput

Screen thousands of variants per campaign.

Flexible Formats

scFv, Fab, IgG, and antibody fragments.

Technology Platform

Advanced Cell-Free Protein Synthesis

Advanced CFPS platforms optimized for rapid antibody expression and screening.

Mammalian CFPS

Mammalian cell-free systems provide proper disulfide bond formation, authentic glycosylation, and authentic folding for functional antibodies. Ideal for therapeutic antibody development.

Proper Folding Glycosylation

E. coli PURE

The PURE system offers defined components for precise control of translation. Perfect for producing unmodified antibodies and rapid screening of antibody fragments.

Defined System High Yield

Integrated Automation

Automated workflows combine DNA assembly, CFPS expression, and binding assays. Single researcher can screen hundreds to thousands of variants per day.

HTP Screening Automated

Quality Control

SDS-PAGE Purity verification

MS Mass verification

Binding Functional assays

COA Full documentation

Antibody Formats

scFv Single-chain variable fragment

Fab Antigen-binding fragment

IgG Full-length antibodies

VHH Nanobodies

Specifications

Flexible Options for Diverse Needs

Comprehensive specifications to meet your research requirements.

Parameter	Mammalian CFPS	E. coli PURE
Formats	IgG, Fab, scFv, VHH	scFv, Fab, VHH
Scale Range	10 μg - 10 mg	100 μg - 50 mg
Turnaround Time	24-48 hours	4-24 hours
Glycosylation	Authentic human-like	None (aglycosylated)
Throughput	High (automation)	Very high
Best For	Therapeutic screening	Research, rapid screening

Workflow

Streamlined Process from Design to Delivery

Our proven workflow ensures quality and efficiency at every stage.

Design

Sequence optimization

Assembly

DNA template preparation

Expression

Cell-free synthesis

QC

Quality verification

Delivery

Results and data report

Applications

Diverse Applications Across Research

Our cell-free antibody services support research in multiple fields.

Antibody Discovery

Rapid screening of antibody variants for binding affinity and specificity. Our high-throughput CFPS platform enables exploration of large sequence spaces in days rather than months.

Rapid variant screening
Large library screening
Epitope mapping
Specificity profiling

10,000+

Variants per campaign

Protein Engineering

Rapid iteration for antibody humanization, affinity maturation, and format optimization. Express and test hundreds of variants to identify optimal candidates for further development.

Humanization
Affinity maturation
Format optimization
Stability engineering

10x

Faster than CHO

Diagnostic Development

Rapid production of antibody reagents for diagnostic assay development. Cold-chain independent systems enable field deployment in resource-limited settings.

Reagent production
Quality control antibodies
Field deployment
Rapid prototyping

No Cold

Chain Required

Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The cell-free platform dramatically accelerated our antibody discovery. We screened 5,000 variants in one week and identified leads that took months with traditional methods."

Senior Scientist

Biotechnology Company

"We expressed toxic membrane protein antibodies that failed in CHO. The cell-free system delivered functional protein in 24 hours. A major breakthrough for our projects."

Research Director

Pharmaceutical Company

"The mammalian CFPS produced antibodies with authentic glycosylation that functioned in our ADCC assays. Fast, reliable, and excellent technical support."

Lead Researcher

Academic Research Institution

Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

74 Citations

A rapid cell-free expression and screening platform for antibody discovery

Hunt AC, Vögeli B, Hassan AO, Guerrero L, et al. Nature Communications. 2023.

Integrated workflow combining cell-free DNA assembly, CFPS, and AlphaLISA for rapid antibody discovery.

View DOI

85 Citations

Cell-free synthesis meets antibody production: A review

Stech M, Kubick S. Antibodies. 2015.

Review of eukaryotic cell-free translation systems for antibody production and glycoengineering applications.

View DOI

71 Citations

Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system

Yin G, Garces ED, Yang J, et al. mAbs. 2012.

Scalable CFPS production of aglycosylated antibodies and antibody fragments with functional activity.

View DOI

38 Citations

An Integrated In Vivo/In Vitro Protein Production Platform for Site-Specific Antibody Drug Conjugates

Hanson J, Groff D, Carlos A, et al. Bioengineering. 2023.

Hybrid platform combining cell-free expression and in vivo production for ADC development.

View DOI

52 Citations

A Simplified and Robust Protocol for Immunoglobulin Expression in Escherichia coli Cell-Free Protein Synthesis Systems

Cai Q, Hanson JA, Steiner AR, et al. Biotechnology Progress. 2015.

E. coli CFPS system optimized for secretory expression of functional full-length antibodies.

View DOI

FAQ

Frequently Asked Questions

Find answers to common questions about our service.

What is cell-free protein synthesis (CFPS)?

Cell-free protein synthesis uses cell extracts or purified components to produce proteins outside of living cells. The reaction mixture contains all necessary machinery for transcription and translation, allowing protein production without the constraints of cell viability, membrane transport, or toxicity.

What are the advantages of cell-free over cell-based expression?

Cell-free systems offer several advantages: faster turnaround (hours vs days/weeks), ability to express toxic proteins, easier scale-up control, direct access to reaction conditions, and compatibility with unnatural amino acids. They are ideal for screening large numbers of variants.

What antibody formats can you produce?

We support multiple formats including scFv (single-chain variable fragment), Fab (antigen-binding fragment), full-length IgG, and VHH (nanobodies). Our mammalian CFPS platform provides authentic glycosylation for IgG formats, while E. coli PURE system offers rapid production of antibody fragments.

What is the typical turnaround time?

E. coli PURE system: 4-24 hours for antibody fragments. Mammalian CFPS: 24-48 hours for full-length antibodies. High-throughput screening campaigns can deliver functional data on hundreds to thousands of variants within one week.

Are the antibodies functional in downstream assays?

Yes, our cell-free produced antibodies demonstrate functional activity in binding assays, flow cytometry, and cell-based assays. Our mammalian CFPS platform produces antibodies with proper folding, disulfide bonds, and glycosylation, ensuring authentic biological activity.

Can you express difficult or toxic proteins?

One major advantage of cell-free systems is the ability to express proteins that would be toxic to living cells. Membrane proteins, regulatory kinases, antimicrobial peptides, and other challenging targets can be successfully produced using our CFPS platforms without concerns about cell viability.

Ready to Start Your Project?

Get a customized quote for your Cell-Free Antibody Production Service project. Our experts will respond within 24 hours.

No obligation

24h response

Expert consultation

Request a Quote

Cell-Free Antibody Production Services

Why Choose Us

Rapid Screening

Mammalian CFPS

No Toxicity

Why Cell-Free Antibody Production?

Rapid Speed

No Cell Toxicity

Batch Consistency

Cold-Chain Independent

High Throughput

Flexible Formats

Ready to Accelerate Your Antibody Discovery?

Advanced Cell-Free Protein Synthesis

Mammalian CFPS

E. coli PURE

Integrated Automation

Quality Control

Antibody Formats

Flexible Options for Diverse Needs

Streamlined Process from Design to Delivery

Design

Assembly

Expression

QC

Delivery

Diverse Applications Across Research

Antibody Discovery

Protein Engineering

Diagnostic Development

What Our Clients Say

Scientific Foundation

A rapid cell-free expression and screening platform for antibody discovery

Cell-free synthesis meets antibody production: A review

Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system

An Integrated In Vivo/In Vitro Protein Production Platform for Site-Specific Antibody Drug Conjugates

A Simplified and Robust Protocol for Immunoglobulin Expression in Escherichia coli Cell-Free Protein Synthesis Systems

Frequently Asked Questions

Ready to Start Your Project?

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