Gene Knockout Services

Knockout (KO) permanently eliminates gene expression at the DNA level, resulting in complete absence of the target protein. Knockdown (KD) using siRNA/shRNA temporarily reduces gene expression without altering the genome. KO provides definitive loss-of-function evidence and is stable across cell generations, while KD effects are transient (typically 3-7 days) and may have incomplete silencing.

We use multiple validation methods: (1) Sanger sequencing of the targeted region to confirm indels, (2) PCR analysis to detect fragment deletions, (3) Western blot to confirm absence of protein expression, and (4) functional assays where applicable. Every delivery includes Sanger sequencing data and detailed QC reports.

Yes. Our automated optimization platform tests 200+ conditions for each cell line to identify optimal transfection parameters. We have successfully generated knockouts in primary cells, iPSCs, neurons, and other traditionally difficult cell types. Contact us to discuss your specific cell line requirements.

We offer three main strategies: (1) Frameshift knockout - single or multiple sgRNAs create indels causing frameshift mutations, (2) Fragment deletion - dual sgRNAs delete a portion of the coding sequence for complete knockout, and (3) Conditional knockout - Cre-lox system for temporal or tissue-specific gene inactivation.

Standard service delivers validated clonal cell lines (single-cell derived) with confirmed homozygous knockout. We also offer cell pool options for faster delivery when clonal isolation is not required. Cell pools typically achieve >50% knockout efficiency and can be used for initial screening or further clonal selection.

All deliveries include: Sanger sequencing validation, STR (Short Tandem Repeat) authentication to confirm cell line identity, mycoplasma testing to ensure contamination-free cells, and detailed experimental documentation. Optional add-ons include Western blot verification, off-target analysis, and expression stability testing.

Simply submit your target gene and cell line information through our quote request form or contact us directly. Our scientific team will evaluate your requirements and recommend the optimal knockout strategy. We provide free technical consultation to ensure your project design meets research goals.