Synthetic Biology
Strain Engineering for Protease

Strain Engineering for Protease

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Strain Engineering for Protease

CD Biosynsis leverages our extensive experience and technological excellence in synthetic biology to help our customers overcome the challenges of traditional protease production to meet the growing demand for proteases in various fields, including the food industry, chemical industry, and pharmaceutical industry. We also support large-scale, high-efficiency protease production to advance the widespread use of proteases in industrial production.

Advantages of Synthetic Biology-Driven Proteases Production

Proteases represent a large group of different hydrolytic enzymes, usually classified by their site of action, enzyme active site structure and specific reaction mechanisms. They have biochemical and/or physiological roles in many aspects of cellular and organismal functions. However, the development of commercial processes for protease production faces many challenges, among which substrate specificity, enzyme stability and enzyme cost are the main issues faced in industry. In addition, most naturally produced protease have many limitations in industrial applications and need to be modified according to the extreme conditions of the industry. Recent advances in synthetic biology have led to breakthroughs in the development of superior protease-producing microorganisms through genetic and metabolic engineering strategies. Synthetic biology provides engineering ideas and capabilities to dramatically improve the biocatalytic efficiency and enzymatic stability of proteases, thus accelerating the industrial realization of biomanufacturing concepts.

A snapshot of synthetic biology tools used for modification of microbial enzymes.Figure 1. A snapshot of synthetic biology tools used for modification of microbial enzymes. (Shukla P., 2019)

What We Provide

CD Biosynsis' advanced synthetic biology platform and extensive expertise allows us to help our customers develop customized and effective strategies to achieve improved production of proteases.

Featured Services

  • Using recombinant DNA technology to construct recombinant microbial strains to increase protease productivity.
  • Engineering of non-native host strains with the capacity to produce high yields of proteases with desired properties.
  • Optimized production, purification, and characterization of proteases with novel properties, such as detergent stability and collagenolytic activity.
  • Development of nanoparticle-based immobilization strategies to obtain protease with high pH stability thermal stability, and tolerance to organic solvents.
  • Development of high-throughput methods for protease engineering to produce protease variants with improved or novel substrate specificities.


  • Protease-producing microorganisms.
  • High-quality protease products, including acidic, neutral, and alkaline proteases.

How We Can Help

CD Biosynsis offers our expertise and extensive experience in synthetic biology to help our customers develop effective synthetic biology strategies for efficient protease production. Our scientists can develop customized and optimized strategies according to customer requirements for different industrial applications.

Development of Synthetic Biology Chassis for Protease Production

Development of Synthetic Biology Chassis for Proteases Production

Our advanced technologies and extensive experience allow us to develop effective synthetic biology tools and strategies for the engineering and modification of different types of microorganisms as ideal chassis for the production of different types of proteases. We focus on developing proteases with high stability and catalytic efficiency to meet the needs of industrial applications in various extreme environments. The following are the protease-producing microorganisms including bacteria, fungi, and algae that we are capable of developing into synthetic biology chassis. If you are interested in other microbial strains, please fill out the online inquiry form and tell us more about your project.

Aspergillus awamori Aspergillus fumigatus Aspergillus niger Aspergillus oryzae Aspergillus oryzae
Aspergillus saitoi Bacillus clausii Bacillus licheniformis Bacillus stearothermophilus Bacillus subtilis
Bacillus subtilis Clostridium acetobutylicum Conidiobolus coronatus Escherichia coli Exiguobacterium profundum
Lactobacillus brevis Lactobacillus buchneri Lysinibacillus fusiformis Streptomyces avermectnus Shewanella algae

Overcome Challenges in Proteases Production

  • Low catalytic efficiency

    We use our powerful synthetic biology technology platform to analyze the binding efficiency of proteases and their substrates through molecular docking and molecular dynamics simulations to develop proteases with high catalytic efficiency.

  • Low stability at high temperatures and variable pH

    We can engineer microorganisms by mutagenesis and cloning to improve the function and stability of proteases.

  • Relatively high cost

    We use strong promoters to increase the yield of proteases, making large-scale production in bioreactors possible and reducing costs.

Applications of Protease

CD Biosynsis provides customized solutions for synthetic biology-driven protease production to meet a wide range of applications in laboratory and industrial production.

Applications of protease in the medical field

  • Treatment of indigestion with pepsin
  • Treatment of bronchiectasis with acid protease
  • Treatment of vasculitis with elastase

Applications of protease in the laboratory

  • Trypsin can be used in cell culture
  • Proteinase K used as a reagent for molecular biology research
  • Protease reagents for endonuclease and exonuclease cleavage applications

Applications of protease in the manufacturing industry

  • Used in the food processing
  • Used in the leather industry
  • Used in the feed industry
  • Used in the petroleum industry

Want to Learn More?

As a rapidly growing synthetic biology company, CD Biosynsis is committed to helping our customers meet the growing and evolving demand for industrial enzyme production. All of our deliverables will undergo a rigorous quality testing process to ensure the quality and reliability and can be delivered on time. If you are interested in our services or have any further questions, please do not hesitate to contact us.


  1. Shukla P. Synthetic Biology Perspectives of Microbial Enzymes and Their Innovative Applications. Indian J Microbiol, 2019, 59(4): 401-409.
  2. Razzaq A, et al. Microbial Proteases Applications. Front Bioeng Biotechnol, 2019, 7: 110.
Please note that all services are for research use only. Not intended for any clinical use.

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