SsDNA Synthesis represents the synthesis process of single stranded DNA. Single stranded DNA (ssDNA) is a molecule with only one DNA strand. The process of synthesizing single stranded DNA can be achieved through various methods, including in vitro synthesis, DNA replication, and DNA repair. This process can be carried out by synthesizing ssDNA oligos in the laboratory, or by synthesizing ssDNA through DNA replication in cells. SSDNA synthesis has important application value in many laboratory research fields, such as primer synthesis, gene cloning, DNA sequencing, and gene expression.
we specialize in providing high-quality ssDNA synthesis services to researchers and scientists. With our expertise and advanced technologies, we can help you obtain the precise ssDNA molecules you need for your research projects
These applications below showcase the versatility of ssDNA synthesis in various fields, including molecular biology, genetics, diagnostics, and biotechnology.
| Application |
Description |
| Oligonucleotide Probes |
Designing short ssDNA sequences as probes for hybridization-based assays, such as fluorescence in situ hybridization (FISH). |
| Primer Design for PCR |
Synthesizing ssDNA primers for use in polymerase chain reaction (PCR) to amplify specific DNA sequences. |
| Site-Directed Mutagenesis |
Generating short ssDNA sequences for introducing specific mutations into a DNA sequence. |
| Sequencing Templates |
Producing ssDNA templates for DNA sequencing reactions, including Sanger sequencing or next-generation sequencing (NGS). |
| Molecular Cloning |
Designing and synthesizing ssDNA fragments for molecular cloning purposes, such as insertion into plasmids or vectors. |
| Functional Genomic Studies |
Creating ssDNA probes for studying gene expression, RNA structure, or interactions with other molecules. |
| Antisense Oligonucleotides |
Developing ssDNA sequences that are complementary to specific mRNA targets for gene silencing or modulation of gene expression. |
| Diagnostic Assays |
Designing ssDNA probes or primers for diagnostic tests, such as detecting specific pathogens or genetic mutations. |
| Protein Binding Studies |
Generating ssDNA sequences for studying protein-DNA interactions in vitro or in vivo. |
| Nucleic Acid Aptamers |
Developing ssDNA sequences with specific binding properties for applications in biosensors or targeted drug delivery. |
| Library Construction |
Constructing libraries of diverse ssDNA sequences for various screening or selection processes, such as in phage display or aptamer selection. |
Service Process
Case Studies
FAQs
Service Process
We have a streamlined service process to ensure a smooth and efficient experience for our clients. Here are the steps involved in our ssDNA synthesis service:
- Consultation: Our team will work closely with you to understand your research requirements and design the ssDNA sequence accordingly. We value your input and strive to create ssDNA molecules that meet your specific needs.
- Synthesis: Using advanced synthesis techniques, we will synthesize the desired ssDNA molecule with high accuracy and efficiency. Our experienced scientists and technicians will carefully handle each step of the synthesis process to ensure the quality and integrity of the synthesized ssDNA.
- Quality Control: Each synthesized ssDNA product undergoes stringent quality control measures to ensure its integrity and purity. We employ a range of quality control techniques and tests to verify the accuracy and reliability of the synthesized ssDNA molecules.
- Delivery: Once the synthesis and quality control processes are complete, the synthesized ssDNA will be securely packaged and delivered to your specified location within the agreed timeline. We take great care in packaging and handling to ensure the stability and integrity of the ssDNA molecules during transportation.
To learn more about our ssDNA synthesis services or to discuss your specific project requirements, please contact us. Our dedicated team is ready to answer any questions you may have and provide you with the necessary information to get started on your ssDNA synthesis project.
Case Studies
Binding of HmtSSB to preformed ssDNA molecules. (A) Schematic of the experimental setup. The complementary strands of a DNA hairpin (2540 bp) were tethered between an optically trapped bead (gray) and a bead on top of a micropipette (black) (Materials and Methods). Mechanical unwinding of the DNA hairpin in the presence of a 30-mer oligonucleotide (Oligont, red line) complementary to the loop end prevents re-winding of the hairpin at low forces, providing a method to manipulate long ssDNA molecules. At constant force (∼3 pN), wrapping of ssDNA by HmtSSB decreases the distance between the beads (x). The final product of the reaction is a 5080-nt long ssDNA–HmtSSB complex. Alternatively, a DNA hairpin containing a dsDNA handle at the 3 end was used to measure the FECs at 300 mM NaCl and 50 mM NaCl/4 mM MgCl2 (Materials and Methods). (B) Independent traces showing the change in extension (x in nm/nt) with time due to protein binding to the ssDNA (50 nM HmtSSB and 50 mM NaCl). Traces were shifted along the time axis for clarity of display. (C) Experimental FECs of protein–DNA complexes assembled under varying protein concentrations, salt concentrations and type. For all panels red and blue curves correspond to the stretching FECs of protein–DNA complexes assembled at the indicated protein concentrations (nM). Two FECs per protein concentration condition are shown. Light red and light blue curves correspond to the initial ssDNA FECs under each condition. Dashed black line corresponds to the WLC model for ssDNA elasticity with a persistence length of 0.75 nm and stretch modulus of 800 pN.
(JA Morin, et al.,2017)
FAQs
Here are some frequently asked questions about our ssDNA synthesis services:
Q: What is the typical turnaround time for ssDNA synthesis?
A: Our standard turnaround time for ssDNA synthesis is [X] business days. However, this may vary depending on the complexity and scale of the project. We strive to provide fast turnaround times without compromising on the quality of the synthesized ssDNA.
Q: Can you synthesize ssDNA with modified nucleotides?
A: Yes, we have extensive expertise in synthesizing ssDNA molecules with various modifications, including modified nucleotides. If you have specific requirements for modified ssDNA, please contact us to discuss your project in detail.
Q: Do you provide purification services for synthesized ssDNA?
A: Yes, we offer purification services to ensure the highest quality ssDNA molecules. Our purification methods remove impurities and unwanted contaminants, resulting in pure ssDNA products. We understand the importance of purity in research applications and take the necessary steps to provide you with clean ssDNA molecules.
Q: Are your ssDNA synthesis services suitable for large-scale projects?
A: Absolutely! Our ssDNA synthesis services are scalable and can accommodate both small-scale and large-scale projects. We have the capacity to fulfill varying project sizes and volumes. Whether you need a few milligrams or several grams of synthesized ssDNA, we can meet your requirements.
We understand that every research project is unique, and we are committed to providing you with customized ssDNA synthesis services that meet your specific needs. Contact us today to discuss your project requirements and find out how we can support your research endeavors.
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