NGS Oligos Synthesis Service

NGS Oligos, encompassing specialized adapters, primers, and index sequences, are the foundational chemical components required for preparing samples for all major Next-Generation Sequencing (NGS) platforms (Illumina, Ion Torrent, PacBio, Oxford Nanopore). The success of complex NGS applications, such as Single-Cell Sequencing, ChIP-Seq, and Ultra-Deep Sequencing, critically depends on the quality and purity of these synthetic oligonucleotides.

CD Biosynsis offers a specialized NGS Oligos Synthesis Service dedicated to meeting the extremely high standards of sequence fidelity and purity required by genomic research. We employ proprietary synthesis and purification technologies (including stringent HPLC and PAGE) to ensure the elimination of truncated sequences, contaminants, and cross-contamination between barcoded adapters. Our comprehensive offering includes standard sequencing adapters, custom dual-index systems, and unique molecular identifiers (UMIs) , all manufactured under strict quality control protocols to guarantee maximum library conversion efficiency and artifact-free sequencing data.

Highlights

Our commitment to NGS Oligos quality ensures high-yield, artifact-free sequencing data:

• Ultra-High Purity: Mandatory HPLC or PAGE purification to remove truncated and failed sequences, which are common causes of chimeric reads and library artifacts.
• Minimal Cross-Contamination: Specialized production lanes and rigorous cleaning protocols eliminate index and barcode cross-talk, crucial for accurate multiplexing.
• Customization Expertise: Ability to synthesize complex structures like single-stranded DNA (ssDNA) adapters, 5' or 3' modifications (e.g., phosphorylation, biotin), and proprietary indexing formats.
• Batch Consistency: Strict SOPs and QC ensure that all oligos, especially high-volume index sets, maintain minimal batch-to-batch variation in concentration and function.

Applications

Reliable NGS oligos are fundamental for achieving high-quality data in cutting-edge genomic research:

Sequencing Library Construction

           

Synthesis of paired-end and single-end adapters for all commercial library preparation kits (Illumina, MGI, PacBio).

High-Throughput Multiplexing

Production of validated dual-index systems (e.g., i5/i7) and large, pre-pooled sets of barcodes for highly multiplexed sequencing runs.

Single-Cell & Ultra-Deep Sequencing

Synthesis of highly complex oligo combinations, including UMIs (Unique Molecular Identifiers) for mitigating PCR bias and improving quantification.

Targeted Sequencing & FISH Probes

Synthesis of capture probes for targeted DNA sequencing (e.g., exome) and long, high-purity FISH probes for genome visualization.

Products & Solutions

A specialized portfolio of high-purity NGS-grade oligonucleotides and accessories.

Sequencing Adapters (Single & Paired-End)

Synthesis of DNA or RNA adapters, available in both double-stranded and specialized single-stranded T-tailed formats.

Custom Index & Barcode Sets (UMIs)

Synthesis of pre-mixed, high-purity i5/i7 index sets and randomized UMI tags for molecular counting applications.

Phosphorylation & Chemical Modifications

Essential 5' modifications (e.g., phosphorylation) are performed using high-yield methods and confirmed by QC for efficient ligation.

RNase-Free & Endotoxin-Controlled Prep

Oligos are prepared and packaged in an RNase-free environment, with optional endotoxin testing suitable for cell-based NGS assays.

High-Volume & Automated Pooling

Capability for synthesizing and precisely pooling hundreds of unique barcodes in a single, ready-to-use mixture for high-throughput screens.

Workflow

Our specialized workflow is designed to eliminate contaminants and maximize the performance of your NGS oligos:

• Design Review and Synthesis: Client submits oligo sequences (adapters, indexes, UMIs). Synthesis is conducted on dedicated synthesizers to prevent cross-contamination, especially among barcode sets.
• High-Stringency Purification: Unlike standard desalted oligos, all NGS oligos undergo HPLC or PAGE purification to ensure minimal levels of N-1 truncated sequences and other contaminants.
• Ligation-Function QC (Optional): Critical modifications (e.g., 5' P) are confirmed by MS. Optional functional QC tests can be performed to verify ligation and PCR efficiency for specific NGS applications.
• Precise Quantification and Pooling: Each oligo is quantified rigorously by UV Spectrophotometry. For multiplexing, sets are precisely pooled into a single mix with equimolar concentration accuracy (pm 2%).
• Final Delivery: Oligos are delivered in an RNase/DNase-free environment with a comprehensive COA including Mass Spec and HPLC purity traces, ensuring compliance with strict NGS standards.

We provide critical assurance for your high-stakes sequencing projects:

• Data Integrity Guarantee: High purity minimizes index hopping, chimeric reads, and sequencing artifacts, leading directly to higher quality, reliable data.
• Cross-Contamination Avoidance: Dedicated process flow for barcodes ensures minimal read misassignment, a common and severe problem in multiplexed sequencing.
• Expert Consultation: Access to specialists for troubleshooting complex UMI design, adapter chemistries, and compatibility with proprietary NGS kits.
• Scalability: Ability to support small research projects as well as large-scale, automated projects requiring thousands of unique UMI sequences or high-volume pre-pooled indexes.