Site-Specific Protein Labeling Service

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Site-specific protein labeling services provide precise and efficient solutions for tagging proteins at specific locations, enabling researchers to study protein function, interactions, localization, and dynamics with high resolution. This advanced technique allows for targeted modification of proteins, enhancing the accuracy and utility of experimental outcomes in various research and biotechnological applications. Our site-specific protein labeling services offer comprehensive support from project design to validation, ensuring that your labeled proteins meet your specific needs.

Site-Specific Protein Labeling via Sortase-Mediated Transpeptidation(MWL Popp, et al.,2009)

Overview Service Process Examples and Solutions Applications Frequently Asked Questions

Overview

Site-specific protein labeling involves the targeted attachment of labels, such as fluorescent dyes, biotin, or other probes, to specific amino acid residues within a protein. This method ensures that the label is precisely placed, allowing for accurate detection and analysis. Our services utilize advanced labeling techniques, including chemical conjugation, enzymatic tagging, and genetic encoding, to achieve high specificity and efficiency in protein labeling.

Service Process

The process of site-specific protein labeling involves several critical and interrelated steps:

Project Consultation: Collaborating with researchers to define the specific labeling requirements, including the type of label, target protein, and intended application.
Label Design and Selection: Choosing the appropriate labeling strategy and selecting the optimal label based on the protein's structure and experimental needs.
Protein Expression and Purification: Producing and purifying the target protein in a suitable expression system to ensure high purity and yield.
Site-Specific Labeling: Applying chemical, enzymatic, or genetic encoding techniques to attach the label at the desired site within the protein. This step ensures high specificity and efficiency.
Purification of Labeled Proteins: Isolating the labeled protein using techniques such as affinity chromatography, size-exclusion chromatography, or ion exchange chromatography to ensure high purity and specificity.
Characterization and Validation: Assessing the labeled protein's purity, labeling efficiency, stability, and functionality using methods such as SDS-PAGE, Western blotting, mass spectrometry, and activity assays.
Optimization and Scale-Up: Refining the labeling process based on initial results and scaling up production to meet the required quantities for research or commercial use.

For more information about our Site-Specific Protein Labeling Services or to discuss your specific needs, please contact us. Our team of experts is available to provide guidance and support for your research and biotechnological projects, ensuring you achieve your scientific and industrial goals.

Examples and Solutions

The following table provides an overview of various case studies in site-specific protein labeling and the solutions we offer to support your research and biotechnological endeavors:

Case Study	Description	Solutions We Offer
Fluorescent Protein Labeling for Imaging	Labeling proteins with fluorescent dyes to study their localization in live cells.	Custom labeling, optimization of labeling conditions, and validation.
Biotinylation for Interaction Studies	Site-specific biotinylation of proteins to facilitate streptavidin-based pulldown assays.	Biotin conjugation, protein purification, and functional assays.
Single-Molecule FRET Studies	Labeling proteins with donor and acceptor fluorophores for single-molecule FRET analysis.	Dual labeling, optimization, and single-molecule analysis.
Enzyme Activity Studies	Site-specific labeling of enzymes to study conformational changes and activity.	Label design, conjugation, and activity assays.
Therapeutic Protein Labeling	Labeling therapeutic proteins to track their distribution and efficacy in vivo.	Custom labeling strategies, in vivo validation, and pharmacokinetic analysis.
Diagnostic Probe Development	Developing labeled proteins for use in ELISA and other diagnostic assays.	Custom labeling, assay development, and validation.

Applications

The applications of site-specific protein labeling are diverse and impactful, including:

Protein Localization Studies: Tracking the precise location of proteins within cells or tissues using fluorescent tags.
Protein-Protein Interaction Analysis: Investigating interactions between proteins using techniques such as Förster Resonance Energy Transfer (FRET) or co-immunoprecipitation.
Single-Molecule Studies: Observing the behavior of individual protein molecules in real-time.
Structural Biology: Enhancing the study of protein structure and conformational changes through targeted labeling.
Drug Development: Screening and validating potential drug candidates by labeling target proteins.
Diagnostic Assays: Developing labeled proteins for use in sensitive and specific diagnostic tests.

Frequently Asked Questions

Q: What is site-specific protein labeling?

A: Site-specific protein labeling involves attaching labels, such as fluorescent dyes or biotin, to specific amino acid residues within a protein. This method ensures precise placement of the label, allowing for accurate detection and analysis of the protein's function and interactions.

Q: How is site-specific protein labeling performed?

A: Site-specific protein labeling is performed through a series of steps including project consultation, label design and selection, protein expression and purification, site-specific labeling, purification of labeled proteins, characterization and validation, and optimization and scale-up. Each step ensures the precise and efficient labeling of proteins.

Q: What are the applications of site-specific protein labeling?

A: Applications include protein localization studies, protein-protein interaction analysis, single-molecule studies, structural biology, drug development, and diagnostic assays. Labeled proteins are essential tools for various research and biotechnological applications.

Q: What are the key steps in the site-specific protein labeling process?

A: Key steps include project consultation, label design and selection, protein expression and purification, site-specific labeling, purification of labeled proteins, characterization and validation, and optimization and scale-up. These steps ensure the successful production of high-quality labeled proteins.

Q: Why is site-specific protein labeling important?

A: Site-specific protein labeling is important for advancing research, developing new therapies, creating diagnostic tools, and improving industrial processes. It provides essential tools for studying and manipulating proteins with high precision in various biological and biotechnological contexts.

Please note that all services are for research use only. Not intended for any clinical use.

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