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Strain Engineering for Glutamate

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CD Biosynsis has established a powerful synthetic biology platform for improving the economic production of glutamic acid and its derivatives and leading the way to sustainable manufacturing. Our experienced scientists can help our customers develop efficient strategies for glutamic acid-producing microorganism improvement and process optimization to meet the growing global demand for glutamic acid.

Advantages of Synthetic Biology-Driven Glutamic Acid Production

Glutamic acid with the molecular formula C5H9NO4 is a nonessential amino acid found in animal and plant proteins. Its ionic form, known as glutamate, plays a critical role in neural activation as a neurotransmitter. The glutamic acid market has been growing and continues to expand due to the wide range of applications of glutamic acid and its derivatives in various industries such as food and pharmaceuticals. Traditional fermentation process can be affected by many factors such as permeability, substrate concentration, carbon source, nitrogen source, medium pH, temperature, growth factors, and oxygen supply. Synthetic biology approaches based on engineering concepts allow the construction of microbial cell factories under precise control to reprogram cellular properties and provide access to novel low-cost carbon sources. The rapid development of synthetic biology has opened up new avenues for improving the microbial production of glutamic acid and its derivatives.

Figure 1. Systems metabolic engineering of C. glutamicum for the production of L-glutamate. (Ma Q, et al., 2017)Figure 1. Systems metabolic engineering of C. glutamicum for the production of L-glutamate. (Ma Q, et al., 2017)

What We Provide

Leveraging our powerful synthetic biology platform, CD Biosynsis can provide our customers with custom synthetic biology services to help them develop effective strategies to achieve efficient biosynthesis of glutamate.

Featured Services

  • Heterologous expression of key enzymes such as xylose isomerase to achieve diversified utilization of carbon sources.
  • Analysis and engineering of microbial metabolic pathways of glutamate and γ-PGA synthesis for accumulation enhancement.
  • Functional design of mechanosensitive channels for improved glutamate excretion efficiency.
  • Design and use of synthetic sRNA systems for microbial metabolic engineering to enhance glutamate production.


  • Glutamic acid-producing microorganisms and γ-PGA-producing microorganisms.
  • High-quality glutamic acid and its derivatives, including but not limited to L-glutamic acid, β-glutamic acid, D-glutamic acid, and γ-PGA.

How We Can Help

CD Biosynsis has been exploring microbial metabolic pathway for glutamic acid production and focused on the biosynthetic mechanism. We have the ability to use synthetic biology techniques to improve productivities of glutamic acid-producing microorganisms or to design and create innovative metabolic pathways to improve the yield and efficiency of the biosynthetic processes. By construction of synthetic biology-powered microbial cell factories for efficient production of glutamic acid and its derivatives, we are committed to meeting our customers' needs in an environmentally sustainable and cost-effective way.

Synthetic Biology-Driven Production of Glutamic Acid/Glutamate

We have developed powerful synthetic biology toolboxes for different species of microorganisms, including but not limited to omics tools, computational tools, genome-wide editing tools, synthetic regulatory tools for microbial engineering, and metabolite biosensors. We have expertise and experience to develop effective strategies for engineering of glutamic acid-producing microorganisms to increase productivity and yields. We can also help isolate and characterize novel microbial strains with ability to secrete high concentration of glutamic acid and tolerance to high glutamic acid concentration. The following is a collection of glutamic acid-producing microorganisms.

Corynebacterium glutamicum Corynebacterium acetoacidophilum Corynebacterium lilum Corynebacterium callunae
Corynebacterium herculis Escherichia coli Brevibacterium flavum Pseudomonas reptilivora
Lactobacillus plantarum Micrococcus glutamicus Brevibacterium aminogenes Brevibacterium divaricatum
Brevibacterium lactofermentum Brevibacterium roseum Brevibacterium saccharolyticum Brevibacterium immariophilium

Synthetic Biology-Driven Production of Poly-γ-Glutamic Acid

Poly-γ-glutamic acid (γ-PGA) is a natural occurring biodegradable biopolymer made up of D/L-glutamic acids. We can help develop synthetic biology strategies for improving γ-PGA production including modifying microbial biosynthetic pathways, enhancing the production of glutamic acid as the precursor for γ-PGA synthesis, and decreasing competitive byproduct production. The microorganisms that have the potential to be developed as synthetic biology chassis for γ-PGA production are listed below. We are also working to discover microorganisms capable of vigorous γ-PGA biosynthesis from renewable feedstocks to help our customers develop sustainable production processes and reduce costs.

Bacillus subtilis Bacillus licheniformis Bacillus velezensis Bacillus natto
Bacillus methylotrophicus Bacillus anthracis Bacillus thuringensis Bacillus cereus
Bacillus pumilus Bacillus amyloliquefaciens Bacillus mojavensis Bacillus atrophaeus
Bacillus megaterium Fusobacterium nucleatum Corynebacterium glutamicum Escherichia coli
Enterococcus faecium Staphylococcus epidermidis Natrialba aegyptiaca Lysinibacillus sphaericus

* If you are interested in other microorganisms or other derivatives of glutamic acid, please fill out the online inquiry form and tell us more about your project.

Applications of Glutamic Acid

As a rapidly growing and customer-focused synthetic biology company, CD Biosynsis offers customized services to help our customers in different fields achieve sustainable production of glutamic acid and its derivatives.

  • Used as a flavor enhancer and food additive in the food industry.
  • Used in food packaging materials.
  • Used as an additive in feed supplements.
  • Used as a precursor for peptide and protein synthesis.
  • Used as an intermediate in organic synthesis and agrochemicals.
  • Used as a plant biostimulant in the agricultural industry.
  • Used as a drug carrier and vaccine adjuvant in the pharmaceutical industry.
  • Used as a hydrophilic humectant in cosmetic industry.

Want to Learn More?

As a rapidly growing synthetic biology company, CD Biosynsis is committed to helping our customers meet the growing and evolving demand for amino acid production. All of our deliverables will undergo a rigorous quality testing process to ensure the quality and reliability and can be delivered on time. If you are interested in our services or have any further questions, please do not hesitate to contact us.


  1. Ma Q, et al. Systems metabolic engineering strategies for the production of amino acids. Synthetic and systems biotechnology, 2017, 2(2): 87-96.
  2. Hirasawa T, Shimizu H. Glutamic acid fermentation: discovery of glutamic acid-producing microorganisms, analysis of the production mechanism, metabolic engineering, and industrial production process. Industrial biotechnology: products and processes, 2016.

Please note that all services are for research use only. Not intended for any clinical use.

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