Engineering of Phage Chassis for Biosynthesis

Genome minimization involves the targeted removal of non-essential genes from phage genomes, typically those encoding hypothetical proteins with unknown functions. This process creates streamlined phage chassis with up to 48% reduced genome size, providing ample space for integration of biosynthetic pathways, therapeutic payloads, or additional functional modules while maintaining essential replication and infection functions.

We offer multiple CRISPR systems including Cas9 for standard DNA editing, Cas12a (Cpf1) which shows efficient cleavage of modified genomes including those with cytosine hydroxymethylation, and Cas13a for RNA targeting in jumbo phages with proteinaceous nuclei. The choice of system depends on your specific phage target and editing requirements.

All engineered phage chassis undergo rigorous verification including whole genome sequencing (NGS) to confirm intended modifications, plaque assay for titer verification, efficiency of plating (EOP) analysis, and phenotypic characterization. A comprehensive Certificate of Analysis is included with every delivery.

Yes, we support engineering projects targeting a wide range of bacterial hosts including ESKAPE pathogens (Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter species), as well as model organisms like E. coli and Bacillus species. Host range engineering via receptor binding protein modification is also available.

Absolutely. Our platform supports seamless integration of biosynthetic gene clusters and metabolic pathways into minimized phage chassis. We work with you to optimize pathway expression, ensure genetic stability, and verify functional output. This service is particularly valuable for natural product discovery and pharmaceutical intermediate production.

Safety is paramount. All engineered phages undergo thorough genomic analysis to confirm absence of toxin genes, virulence factors, and antibiotic resistance determinants. For therapeutic applications, we can implement additional safety features including lysis-deficient designs and genome containment systems. We comply with relevant biosafety guidelines and provide documentation for regulatory submissions.

Simply click the "Get Free Quote" button to initiate the consultation process. Our technical team will review your requirements and contact you within 1-2 business days to discuss your project goals, timeline, and provide a customized quote. We offer complimentary initial consultations to help define project scope and feasibility.