CD Biosynsis is able to use metabolic engineering and genetic engineering to alter the metabolic flow of flavonoids, expand their metabolic pathways, and construct new metabolic pathways to modify the metabolic purpose of cells to meet the specific needs of humans with flavonoid production.
Flavonoids are widely distributed in the plant world, and more than 2,000 kinds of natural flavonoids have been found. Flavonoid compounds are secondary metabolites of the flavonoid biosynthetic pathway and are highly abundant in the flowers and leaves of plants. Anthocyanins are one of the end products of flavonoid metabolism. Flavonoids have various effects such as antibacterial, anti-inflammatory, and antioxidant. The research, development, and application of flavonoids have become an important direction in the fields of food, medicine, chemical, and biological engineering. As the application of flavonoid compounds continues to expand, flavonoids produced from plant extracts are no longer able to meet the market demand due to the influence of plant growth. Therefore, the use of synthetic biology can enable the large-scale production of flavonoids.
Figure 1. The flavonoid biosynthetic pathway in plants contains eight branches and four important intermediate metabolites. (Liu W, et al., 2021)
With the development and maturation of proteomic, genomic, and transcriptomic analysis technologies in exploring the molecular mechanisms of plant secondary metabolism, we are able to regulate the flavonoid production process using metabolic and genetic engineering to achieve large-scale production of flavonoids without plant growth cycle limitations.
Development of Microbial Chassis for Flavonoid Production
Microorganisms are fast-growing, easy to culture, and suitable for large-scale fermentation production. The synthesis of flavonoids using microorganisms has high feasibility and use value. We are able to express flavonoid synthesis-related genes in microorganisms and carry out fermentation and achieve large-scale and industrial production of flavonoids through the optimization of strain selection and fermentation condition control. The following are the microbial chassis that has been used for the production of flavonoids. Please contact us directly if you have other chassis of interest for flavonoid production.
|Escherichia coli||Saccharomyces cerevisiae||Symphoricarpos albus||Corynebacterium glutamicum|
|Yarrowia lipolytica||Lactococcus lactis||Pichia pastoris|
Microbial Co-Culture for Flavonoid Synthesis
Based on the well-defined molecular mechanism of the flavonoid synthesis pathway, we are able to produce flavonoids by modular co-culture of two or more microorganisms. Through the co-culture system, we divide the flavonoid synthesis pathway into different modules to provide better reaction conditions for the key enzymes in the synthesis pathway, reduce the metabolic burden of microbial strains, and improve the efficiency of flavonoid production. We are able to improve strains using physical mutagenesis, chemical mutagenesis, and conformational mutagenesis. The following table shows the co-cultivation system we have successfully built. If you would like to build other strain combinations or more than two microbial co-culture systems, you can contact us directly or fill out the online form to let us know and we will be happy to explore new co-culture systems with you.
|Escherichia coli–Saccharomyces cerevisiae||Escherichia coli–Escherichia coli||Saccharomyces cerevisiae–Saccharomyces cerevisiae|
CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive flavonoid production and meet the needs of customers in a variety of industries.
CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in flavonoids production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.