Trusted by Leading Research & Pharma Institutions

CRISPR/Cas9 Knockout Libraries

Accelerate your functional genomics research with genome-wide CRISPR/Cas9 knockout libraries. Our comprehensive platform delivers high-quality sgRNA libraries with validated efficiency for robust genetic screening in drug discovery and target validation.

Genome-Wide Coverage

NGS Verified

High Knockout Efficiency

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Trusted by leading research and pharmaceutical institutions

Stanford

Roche

MIT

Pfizer

Johns Hopkins

Novartis

Why Choose Our Libraries

Validated sgRNA designs with high on-target activity

Comprehensive genome coverage with multiple sgRNAs per gene

Rigorous quality control with NGS verification

20K+

Genes Covered

4-6

sgRNAs per Gene

≥90%

Knockout Efficiency

98%+

Library Coverage

Service Overview

Comprehensive CRISPR/Cas9 Knockout Library Solutions

Our CRISPR/Cas9 knockout libraries enable genome-wide loss-of-function screening with validated efficiency and comprehensive coverage for drug discovery and functional genomics research.

Pooled Lentiviral Libraries

High-titer lentiviral pooled libraries enable efficient transduction into a wide range of cell types including difficult-to-transfect and primary cells. Each library undergoes rigorous quality control with NGS verification to ensure uniform representation.

Genome-wide and targeted subpool options
Multiple sgRNAs per gene for robust knockout
Lentiviral delivery for broad cell type compatibility

Arrayed Library Screening

Arrayed formats enable high-content phenotypic screening without the need for NGS deconvolution. Ready-to-transfect synthetic guides simplify workflows and accelerate discovery timelines.

Binary and multiparametric functional assays
Standard PCR/Sanger quantification
Flexible plate formats available

Validated sgRNA Design

Computationally optimized sgRNAs with proven on-target activity and minimized off-target effects using established design algorithms.

NGS Quality Verification

Every library undergoes next-generation sequencing verification with detailed QC reports for complete transparency.

Custom Library Design

Flexible options for genome-wide, pathway-focused, or user-defined gene sets to match your research objectives.

Technology Platform

Advanced CRISPR Knockout Technologies

State-of-the-art platforms delivering high-efficiency knockout with validated performance.

Validated sgRNA Design

Optimized sgRNA designs using established algorithms including Rule Set 2/3 for enhanced on-target activity. Multiple guides per gene ensure robust knockout efficiency.

Rule Set 2/3 CFD Scoring

High-Efficiency Delivery

Advanced lentiviral packaging systems achieve high-titer preparations (≥10^8 TU/mL) for efficient transduction across diverse cell types including primary cells.

≥10^8 TU/mL High Titer

Rigorous QC Standards

Comprehensive quality control with NGS verification ensures library uniformity, coverage, and representation across all preparations.

NGS Verified 98%+ Coverage

Multi-Guide Design Strategy

Our libraries employ a multi-guide design strategy with 4-6 sgRNAs per gene to ensure robust and consistent knockout efficiency. This approach addresses variability in sgRNA performance and provides redundant coverage for confident hit identification.

Higher probability of effective knockout per gene
Redundancy against sgRNA efficiency variation
Improved statistical power for screening

4-6

sgRNAs per Gene

Higher Confidence Better Coverage

Specifications

Product Specifications

Comprehensive specifications for our CRISPR knockout library products.

Library Types

Human Whole Genome

• 20,000+ protein-coding genes

• 76,000-123,000 sgRNAs

• 4-6 sgRNAs per gene

Mouse Whole Genome

• 20,000+ protein-coding genes

• 86,000+ sgRNAs

• 4-6 sgRNAs per gene

Targeted Subpools

• Kinases, GPCRs, Transcription Factors

• Epigenetic regulators

• Custom gene sets available

Technical Parameters

Vector System

• 3rd generation lentiviral vectors

• All-in-one or two-vector options

• Multiple selection markers

Quality Standards

• Lentiviral titer: ≥10^8 TU/mL

• Library coverage: ≥98%

• NGS verification included

Delivery Formats

• Plasmid DNA

• Lentiviral particles

• Arrayed plates (384-well)

Species Coverage

Species	Gene Coverage	sgRNA Count	Format
Human	20,000+	76,000-123,000	Plasmid / Virus / Arrayed
Mouse	20,000+	86,000+	Plasmid / Virus / Arrayed
Rat	Available	Custom	Plasmid / Virus
Custom Species	On request	Custom	Custom

Workflow

Screening Workflow

A streamlined end-to-end process from library delivery to hit identification.

Step 1

Library Preparation

Receive high-titer lentiviral library with NGS QC report. Amplify library if needed following our validated protocol.

Step 2

Cell Transduction

Transduce Cas9-expressing cells at low MOI (0.3-0.6) to ensure single sgRNA per cell. Maintain 200-500x coverage.

Step 3

Selection & Screening

Apply phenotypic selection (drug treatment, FACS, survival) based on your experimental design.

Step 4

NGS & Analysis

Extract genomic DNA, amplify sgRNA regions, and perform Illumina NGS for sgRNA abundance quantification.

Step 5

Hit Identification

Bioinformatic analysis using MAGeCK or similar tools to identify enriched/depleted genes from screen data.

Applications

Diverse Applications Across Research

Our CRISPR knockout libraries support research and development across multiple fields.

Drug Discovery & Target Identification

Identify and validate therapeutic targets through genome-wide loss-of-function screening. Our libraries enable systematic interrogation of genetic dependencies for novel drug target discovery.

Synthetic lethality screening
Drug resistance mechanism discovery
Target validation and prioritization
Biomarker identification

20K+

Genes Screened Simultaneously

Oncology Research

Discover cancer vulnerabilities and resistance mechanisms through systematic genetic screening. Identify essential genes and synthetic lethal interactions for therapeutic development.

Cancer dependency mapping
Combination therapy discovery
Tumor microenvironment studies
Immunotherapy target discovery

Comprehensive

Cancer Cell Line Compatibility

Functional Genomics

Systematically characterize gene function across the genome. Our libraries enable comprehensive functional annotation for understanding biological pathways and cellular mechanisms.

Essential gene identification
Pathway analysis
Genetic interaction mapping
Cellular phenotype characterization

High-Resolution

Genome-Wide Functional Analysis

Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The library quality exceeded our expectations. Consistent coverage and uniform distribution across all samples. NGS verification reports were comprehensive and helped us validate our screening results."

Senior Scientist

Pharmaceutical Company

"The multi-guide design significantly improved our hit detection rate. Customer support was responsive and provided valuable technical guidance throughout our screening project."

Research Director

Academic Research Institution

"Successfully identified novel drug targets in our oncology screening. The library coverage and delivery format made the entire workflow straightforward and efficient."

Principal Investigator

Biotechnology Company

Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

316 Citations

CRISPR-Cas9 Library Screening Approach for Anti-Cancer Drug Discovery: Overview and Perspectives

Chan YT, Lu Y, Wu J, Zhang C, et al. Theranostics. 2022;12(7):3329-3344.

Comprehensive review of CRISPR-Cas9 pooled library screening technology for anti-cancer drug discovery, covering positive and negative selection methods, experimental models, and target identification applications.

View DOI

42 Citations

Protocol for Performing Pooled CRISPR-Cas9 Loss-of-Function Screens

Mathiowetz AJ, Roberts MA, Morgens DW, Olzmann JA, Li Z. STAR Protocols. 2023;4(2):102201.

Detailed protocol for designing, amplifying, and screening pooled CRISPR-Cas9 knockout libraries in mammalian cells, including phenotypic selection and NGS analysis.

View DOI

28 Citations

CRISPR-Suppressor Scanning for Systematic Discovery of Drug Resistance Mutations

Ngan DK, et al. Current Protocols. 2023.

Framework for performing pooled CRISPR-Cas9 tiling mutagenesis screens with drug selection to identify drug resistance mutations, including sgRNA library design and analysis.

View DOI

15 Citations

Perturbomics: CRISPR–Cas Screening-Based Functional Genomics Approach for Drug Target Discovery

Multiple authors. Experimental & Molecular Medicine. 2025;57:1443-1454.

Review of CRISPR-based perturbomics advances including single-cell analyses, synthetic lethality interactions, and therapeutic target discovery for cancer and other diseases.

View DOI

12 Citations

Genome-Scale CRISPR-Cas9 Screening in Stem Cells: Theories, Applications and Challenges

Multiple authors. Stem Cell Research & Therapy. 2024.

Comprehensive review of CRISPR-based functional genomics screening applications in stem cells, covering library design, screening protocols, and therapeutic applications.

View DOI

FAQ

Frequently Asked Questions

Find answers to common questions about our CRISPR knockout library services.

We offer both pooled lentiviral libraries and arrayed format libraries. Pooled libraries are ideal for genome-wide negative and positive selection screens, while arrayed libraries enable high-content phenotypic screening without NGS deconvolution. We provide human, mouse, and rat genome-wide libraries as well as targeted subpools (kinases, GPCRs, transcription factors, etc.) and custom library design services.

Our standard libraries include 4-6 sgRNAs per gene, designed using validated algorithms (Rule Set 2/3) for optimal on-target activity and minimal off-target effects. This multi-guide approach ensures robust knockout efficiency and provides redundancy against sgRNA efficiency variation, improving statistical power for screening experiments.

All libraries undergo rigorous quality control including: NGS verification to confirm library representation and uniformity (≥98% coverage), functional titer testing for lentiviral preparations (≥10^8 TU/mL), and insert validation by PCR. Each delivery includes a comprehensive QC report documenting these quality metrics.

Yes, we offer comprehensive custom library design services. You can provide specific gene lists for pathway-focused or disease-relevant libraries, or request modifications to existing library formats. Our team can also assist with sgRNA design optimization for unique species or specific genomic targets. Contact us to discuss your custom library requirements.

Our lentiviral libraries are compatible with all cells that can be transduced, including dividing and non-dividing cells, primary cells, and difficult-to-transfect cell types. For optimal results, we recommend using cells that stably express Cas9. We also offer pre-packaged all-in-one systems where Cas9 is included in the viral vector for immediate use.

We provide comprehensive support including Illumina sequencing primer sets optimized for minimal bias, detailed protocols for NGS library preparation, and analysis guidelines using established tools like MAGeCK. Our technical team can also provide consultation on experimental design, statistical analysis approaches, and hit validation strategies.

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Get a customized quote for your CRISPR/Cas9 Knockout Libraries project. Our experts will respond within 24 hours.

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Expert consultation

CRISPR/Cas9 Knockout Libraries

Why Choose Our Libraries

Comprehensive CRISPR/Cas9 Knockout Library Solutions

Pooled Lentiviral Libraries

Arrayed Library Screening

Validated sgRNA Design

NGS Quality Verification

Custom Library Design

Ready to Accelerate Your Research?

Advanced CRISPR Knockout Technologies

Validated sgRNA Design

High-Efficiency Delivery

Rigorous QC Standards

Multi-Guide Design Strategy

Product Specifications

Library Types

Technical Parameters

Species Coverage

Screening Workflow

Library Preparation

Cell Transduction

Selection & Screening

NGS & Analysis

Hit Identification

Diverse Applications Across Research

Drug Discovery & Target Identification

Oncology Research

Functional Genomics

What Our Clients Say

Scientific Foundation

CRISPR-Cas9 Library Screening Approach for Anti-Cancer Drug Discovery: Overview and Perspectives

Protocol for Performing Pooled CRISPR-Cas9 Loss-of-Function Screens

CRISPR-Suppressor Scanning for Systematic Discovery of Drug Resistance Mutations

Perturbomics: CRISPR–Cas Screening-Based Functional Genomics Approach for Drug Target Discovery

Genome-Scale CRISPR-Cas9 Screening in Stem Cells: Theories, Applications and Challenges

Frequently Asked Questions

Ready to Start Your Project?

Recommended Services

Related Resources

Request a Quote

CRISPR/Cas9 Knockout Libraries

Why Choose Our Libraries

Comprehensive CRISPR/Cas9 Knockout Library Solutions

Pooled Lentiviral Libraries

Arrayed Library Screening

Validated sgRNA Design

NGS Quality Verification

Custom Library Design

Ready to Accelerate Your Research?

Advanced CRISPR Knockout Technologies

Validated sgRNA Design

High-Efficiency Delivery

Rigorous QC Standards

Multi-Guide Design Strategy

Product Specifications

Library Types

Technical Parameters

Species Coverage

Screening Workflow

Library Preparation

Cell Transduction

Selection & Screening

NGS & Analysis

Hit Identification

Diverse Applications Across Research

Drug Discovery & Target Identification

Oncology Research

Functional Genomics

What Our Clients Say

Scientific Foundation

CRISPR-Cas9 Library Screening Approach for Anti-Cancer Drug Discovery: Overview and Perspectives

Protocol for Performing Pooled CRISPR-Cas9 Loss-of-Function Screens

CRISPR-Suppressor Scanning for Systematic Discovery of Drug Resistance Mutations

Perturbomics: CRISPR–Cas Screening-Based Functional Genomics Approach for Drug Target Discovery

Genome-Scale CRISPR-Cas9 Screening in Stem Cells: Theories, Applications and Challenges

Frequently Asked Questions

Ready to Start Your Project?

Recommended Services

Related Resources