CD Biosynsis is committed to helping our customers develop synthetic biology strategies to create cost-effective ways to produce ribosomal peptides and break the limitations of ribosomal peptides in traditional production processes. Our expertise in synthetic biology chassis development and metabolic engineering can help our customers overcome all difficulties in the production and application of ribosomal peptides.
Ribosomal peptides or ribosomally synthesized and post-translationally modified peptides (RiPPs) are a large group of natural products synthesized from ribosomes and post-translationally modified, with a wide range of structural and biological activity diversity, which have a wide range of applications in the food industry, pharmaceutical industry, and agriculture fields. Traditional methods for the production of ribosomal peptides are usually based on the solid-phase synthesis, liquid-phase synthesis, and in vivo biotechnological recombination techniques, but this approach possesses many limitations, such as high production costs and low purity of the resulting products. The revolutionary technologies and innovative engineering concepts of synthetic biology have opened a new avenue for the development of microbial platforms for the production of Ribosomal Peptides. Moreover, the industrialization of synthetic biology-driven microbial production processes is an effective way to improve the functional properties of Ribosomal Peptides and reduce the production cost of Ribosomal Peptides.
Figure 1. Peptide bioengineering strategies. (Huang HM, et al., 2019)
CD Biosynsis leverages our advanced technologies in bacterial synthetic biology, fungal synthetic biology, enzyme engineering, genetic engineering, and metabolic engineering to help our customers overcome the challenges of ribosomal peptide production. Our exceptional scientists can provide customized solutions to meet our customers' specific needs for ribosomal peptide production and applications.
We are able to use genetic engineering techniques to genetically manipulate the biosynthetic gene clusters for ribosomal biosynthesis and post-translational modification of peptides to produce new derivatives.
We can use proteomic approaches to drive the mining of new ribosomal peptides by linking the chemotypes of ribosomally synthesized and post-translationally modified peptides to their genotypes.
Based on genome sequencing technology and mass spectrometry platform, we can provide development and identification services for microbial ribosomal synthesis and post-translational modification of peptides.
Heterologous Production of Ribosomal Peptides
We can help our customers design and develop microbial cell factories and reconfigure biosynthetic pathways in the heterologous hosts through the powerful synthetic biology-based genetic manipulation platform and computer-aided technologies for large-scale, efficient ribosomal peptide production. The following is a list of microbial strains that can be used for the heterologous production of ribosomal peptides. If you can't find the strain you are interested in, please fill out the online inquiry form and tell us more about your project.
|Aspergillus niger||Bacillus subtilis||Saccharomyces avermitilis||Saccharomyces coelicolor|
|Bacillus amyloliquefaciens||Escherichia coli||Saccharomyces cerevisiae||Saccharomyces lividans|
Artificial Evolution of Ribosomal Peptides Biosynthetic Pathways
Our scientists have established synthetic promoter libraries to reconstitute biosynthetic pathways for the production of specific ribosomal enzymes based on high-throughput screens. We have optimized the expression of ribosomal peptides by establishing a rational combination strategy of promoters with different intensities to improve the yield of ribosomal peptides.
CD Biosynsis leverages our specialized synthetic biology platform and extensive technical experience to customize synthetic biology-driven ribosomal peptide production solutions for our customers to meet the growing needs in the following areas.
CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in protein and peptide production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.