CD Biosynsis provides professional services for cell-free metabolic engineering to facilitate the cutting-edge research of synthetic biology researchers around the world.
Microbial processes allow for the cleaner and more efficient production of diverse chemicals and natural products to meet the growing demand for sustainability. However, problems including inefficient feedstock conversion, low volumetric yields, accumulation of toxic intermediates, unintentional release of genetically modified microorganisms into the environment, and loss of byproducts through competitive pathways limit the use of bioprocesses that rely on recombinant microorganisms. To overcome these limitations, cell-free metabolic engineering (CFME), a new approach that does not rely on living cells, was developed.
Recently, the freedom relative to in vivo systems has stimulated the development of the engineering fundamentals for cell-free systems. Cell-free synthetic biology enables the engineering of biological components and systems for life science applications without using living cells. It overcomes the inherent limitations of living cells, while also providing simpler and faster engineering solutions with unprecedented freedom in an open environment than a cell system. Cell-free metabolic engineering combined with synthetic biology offers the ability to design metabolic pathways towards the production of desired products.
Figure 1. Paradigms for metabolic engineering. (Dudley Q M, et al., 2015)
CD Biosynsis provides a portfolio of synthetic biology tools for cell-free metabolic engineering. Our services include but not limited to the following:
The physiological constraints required to sustain a living system can limit the conditions for bioconversion using living cells. The use of purified enzymes to build cell-free metabolic pathways is the most straightforward solution to address these limitations. CD Biosynsis offers specialized cell-free metabolic engineering services based on purified enzymes to enable optimization of the participating enzymes.
The requirement for laborious purification of enzymes limits the use of enzyme-based cell-free metabolic engineering. Cell extract-based approaches might be more realistic in cases where the intermediates of the final products can be generated via cellular metabolism. CD Biosynsis provides high-quality cell extract-based cell-free metabolic engineering services to minimize the genetic modification of host cells and the loss of intermediates
CD Biosynsis is fully committed to providing our customers with advanced techniques and tailored one-stop solutions to help them overcome the challenges in synthetic biology. Our scientists have the ability to bring diverse creative ideas to fruition. If you are interested in our services, please feel free to contact us for more details.
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.