Synthetic Biology
Small Genome Synthesis

Small Genome Synthesis

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Small Genome Synthesis

Small Genome Synthesis (SGS) refers to the process of constructing a smaller genome through synthetic methods. It is an artificial synthetic biology technique aimed at assembling genomes into smaller artificial chromosomes or synthetic genomes for genetic engineering or synthetic biology research.

Four design-build-test cycles produced JCVI-syn3.0.(CA Hutchison III,  et al.,2016)Four design-build-test cycles produced JCVI-syn3.0.(CA Hutchison III, et al.,2016)

SGS technology typically involves breaking down DNA sequences into smaller fragments and then combining these fragments together using chemical synthesis methods. These fragments can come from natural genome pruning or synthetic gene fragments designed by computers. With the increase and connection of synthetic fragments, the target genome can ultimately be constructed.

The synthesis of small genomes provides many advantages. Firstly, by constructing smaller genomes, scientists can conduct functional and evolutionary research on microorganisms, understanding how key parts of the genome interact with each other. Secondly, synthetic microgenomes can be used to design and construct specialized microorganisms to produce useful compounds such as drugs and biofuels. In addition, SGS can provide an effective method for genome editing and recombination.

At our company, we offer a comprehensive range of services for small genome synthesis, tailored to meet your specific requirements.

Service Process FAQs

Service Process

Our service process is designed to provide a seamless experience, from initial consultation to the delivery of the synthesized genome. Here's an overview of the steps involved:

  1. Consultation: We begin by working closely with you to understand your project goals, requirements, and specific needs. Our team of experts will collaborate with you to ensure that we have a comprehensive understanding of your desired genome design.
  2. Design and Optimization: Based on the information gathered during the consultation phase, our team will design the genome sequence and optimize it for synthesis efficiency. We take into account factors such as gene function, desired modifications, and compatibility with the host organism.
  3. Synthesis and Assembly: Using advanced techniques and equipment, we synthesize the DNA fragments required for constructing the desired genome. These fragments are then assembled to create the complete genome sequence. Throughout this process, we maintain strict quality control measures to ensure accuracy and integrity.
  4. Quality Control: Once the genome is synthesized and assembled, we implement rigorous quality control measures to verify its accuracy and integrity. Our team conducts thorough testing and analysis to ensure that the synthesized genome meets the highest standards of quality.
  5. Delivery: Finally, we deliver the synthesized genome to you, along with detailed documentation that outlines the sequence, modifications, and any other relevant information. We strive to provide a seamless and hassle-free delivery process, ensuring that you have everything you need to proceed with your research or application.


Here are some frequently asked questions about our small genome synthesis services:

Q: How long does the small genome synthesis process take?

A: The timeframe for small genome synthesis can vary depending on the complexity of the project. During the consultation phase, our team will provide you with an estimated timeline based on your specific project requirements.

Q: Can you synthesize genomes from non-model organisms?

A: Yes, we have expertise in synthesizing genomes from a wide range of organisms, including non-model organisms. Our team of scientists has experience working with diverse species and can adapt our synthesis process to meet your specific needs.

Q: What is the cost of small genome synthesis?

A: The cost of small genome synthesis depends on various factors, including genome size, complexity, and additional customization requirements. We offer personalized quotes based on your specific project, so please contact us for detailed pricing information.

Q: Do you offer post-synthesis support and troubleshooting?

A: Absolutely. We understand that the synthesis process is just the beginning of your journey. Our team provides comprehensive support after synthesis, including troubleshooting and guidance for downstream applications. We are committed to ensuring your success and are available to assist you throughout your project.

Q: Can you assist with genome editing and modification?

A: Yes, we offer genome editing and modification services in addition to small genome synthesis. Our team of experts can help you introduce specific modifications, such as gene knockouts or insertions, in the synthesized genome to meet your research goals.

Q: What types of applications can benefit from small genome synthesis?

A: Small genome synthesis has broad applications across various fields of research and industries. It can be applied in areas such as synthetic biology, genetic engineering, pharmaceutical development, and agricultural biotechnology, among others. The ability to custom-design genomes opens up new possibilities for creating organisms with desired traits and studying gene function.

Q: Is the synthesized genome compatible with different host organisms?

A: Yes, we can design and synthesize genomes that are compatible with a wide range of host organisms. Our team considers the specific requirements and characteristics of the target organism during the design and optimization process to ensure compatibility and functionality.

Q: Can you handle large-scale genome synthesis projects?

A: Yes, we have the capability to handle both small-scale and large-scale genome synthesis projects. Whether you need a single gene synthesized or an entire genome, our team is equipped to deliver high-quality results efficiently.

Q: What level of confidentiality do you provide?

A: We understand the importance of confidentiality in research and development projects. We have strict protocols in place to ensure the protection of your intellectual property and maintain the confidentiality of your project. We are open to signing non-disclosure agreements (NDAs) to provide an extra layer of security and peace of mind.

If you have any other questions or would like to discuss your specific project in more detail, please don't hesitate to contact us. Our team of experts is here to assist you and provide the solutions you need for your small genome synthesis requirements.

Please note that all services are for research use only. Not intended for any clinical use.

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