Synthetic Biology
Semirational Protein Design

Semirational Protein Design

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Semirational Protein Design

CD Biosynsis provides professional semirational protein design services for synthetic biology researchers around the world to facilitate their cutting-edge research.

Introduction to Semirational Protein Design

Protein design tools have become very diverse. As a combination of rational design and directed evolution, semi-rational protein design has been developed to address the limitations of both methods. Information on protein sequence, structure and function is used together with predictive algorithms to identify target amino acid residues. The methodological advances in semirational protein design provide synthetic biology with more powerful and effective strategies. These combinatorial methods have successfully enabled existing proteins to repurpose to adopt new functions.

Figure 1. Overview of approaches for protein engineering by random, rational and combined methods. (Steiner K & Schwab H., 2012)Figure 1. Overview of approaches for protein engineering by random, rational and combined methods. (Steiner K & Schwab H., 2012)

Service Offering

We provide multiple semirational protein design approaches to bring diverse synthetic applications and creative ideas to fruition.

Sequence-Based Approaches

Protein sequences provide important biochemical information. Amino acid sequences can offer insights into the secondary and tertiary structures of proteins as well as their functions. Based on our advanced and innovative sequencing platforms and our expertise in sequence analysis, we provide sequence-based approaches of semirational protein design to meet customer needs.

Structure-Based Approaches

Protein structure determines whether it can interact with other molecules. It is critical to the basic structure of cells and the function of proteins. Structure-based approaches represent important strategies with great potential for protein design. We are committed to providing professional and customized protein design services relying on our integrated platform for the determination of protein structures.

Mutation-Based Approaches

Site saturation mutagenesis (SSM) can be used to establish a mutant library allowing the substitution of specific sites against all 20 possible amino acids at once. We provide mutation-based approaches to help our customers design proteins that meet their specific requirements.

CD Biosynsis offer a variety of cutting-edge approaches of semirational protein design to tailor services for individual customer specifications. We have an experienced scientific team of biologists, bioinformatics, and chemists to help execute innovative designs for unique applications.

We are available to assist you via email or phone with any problems or concerns. If you are interested in our services or have any specific needs, please feel free to contact us for more details.


  1. Steiner K & Schwab H. Recent advances in rational approaches for enzyme engineering. Computational and structural biotechnology journal, 2012, 2(3): e201209010.
  2. Korendovych I V. Rational and semirational protein design. Protein Engineering. Humana Press, New York, NY, 2018: 15-23.
  3. Lutz S. Beyond directed evolution—semi-rational protein engineering and design. Current opinion in biotechnology, 2010, 21(6): 734-743.
Please note that all services are for research use only. Not intended for any clinical use.

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