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Schematic diagram of the biodegradation of toxic recalcitrant compounds.

Biodegradation Pathway Engineering

Synthetic biology has emerged as a promising approach for engineering microorganisms to perform complex metabolic tasks, including biodegradation. Biodegradation pathway engineering involves the modification of microbial metabolism to degrade specific contaminants or pollutants. At CD Biosynsis, we offer a range of services for biodegradation pathway engineering, utilizing the latest synthetic biology tools and techniques to design and optimize microbial consortia for biodegradation applications.

Elimination of the apramycin resistance gene in the host strain genome.

Antibiotic Resistance Marker Removal

The use of antibiotic resistance genes as selection markers in synthetic biology strains has been a common practice for many years. However, the increasing concern over the spread of antibiotic resistance has prompted scientists to develop alternative selection systems. The use of antibiotic-free selection systems is not only a safer option but also more cost-effective in the long run.

The non-recombinant approach to display antigens and enzymes on the surface of bacterial spores.

Spore Engineering

Spore engineering is a cutting-edge technology that has revolutionized the field of synthetic biology. It involves the genetic modification of bacterial spores, which are highly resistant structures that can survive harsh environmental conditions. Spore engineering has various applications in the fields of bioremediation, bioproduction, drug delivery, and biosensing. CD Biosynsis is a leading company in spore engineering, providing innovative solutions to meet the needs of our clients.

A whole-cell biocatalytic strategy was developed to acylate esculin, and the introduction of medium- and long-length fatty acids chains in acylation endowed esculin with non-selective cytotoxicity.

Whole-Cell Biosynthesis

Whole-cell biosynthesis is a rapidly growing field in synthetic biology that involves the use of living cells as a platform for the production of valuable compounds. This approach offers a number of advantages over traditional chemical synthesis, including improved efficiency, reduced waste, and the ability to use renewable resources. CD Biosynsis is a leading company in the field of whole-cell biosynthesis, providing a range of services for the production of a wide variety of compounds using genetically modified microorganisms.

Key advantages of cellular and cell-free bioproduction.

Cell-Free Systems

Cell-free systems are gaining popularity as a platform for protein synthesis due to their rapid and efficient nature. These systems are free from cell walls and membranes and can be used to synthesize proteins directly from DNA templates. They offer several advantages over traditional methods of protein expression, including increased speed, simplicity, and cost-effectiveness. Synthetic biology has played a significant role in advancing cell-free systems by providing new methods for DNA synthesis and assembly, as well as new enzymes and substrates for protein synthesis.

Biocontainment strategies and biosafety guidelines.

Biocontainment Strategies in Strains

In the field of synthetic biology, genetically modified organisms (GMOs) have been engineered to produce a variety of products at an industrial scale. However, concerns have been raised surrounding the ecological risks associated with the release of GMOs outside of controlled environments. Biocontainment systems are needed to neutralize these GMOs and prevent them from causing harm to the environment. In addition, alternative selection markers are needed to replace costly and risky antibiotics.

The abstract formation of an orthogonal DNA replication system by Plasmid/DNA polymerase pair.

Orthogonal Genetic Systems in Strains

In recent years, the field of synthetic biology has made significant advancements in developing new genetic systems that allow for precise and efficient control of gene expression and editing. One promising approach is the use of orthogonal genetic systems, which enable the simultaneous regulation and editing of multiple genes with high specificity and efficiency. CD Biosynsis is leading the way in providing cutting-edge services in this exciting orthogonal genetic systems field.

Overview of protocols of Golden Gate cloning for assembly of multiple DNA fragments.

DNA Assembly and Cloning

DNA assembly and cloning are critical techniques in synthetic biology that enable researchers to create and modify DNA sequences for various applications, including gene editing, protein engineering, and synthetic biology. Traditional cloning methods, such as restriction enzyme digestion and ligation, can be time-consuming and often result in unwanted mutations or errors. However, recent advancements in DNA assembly and cloning techniques have enabled researchers to construct complex DNA sequences with greater speed, accuracy, and efficiency.

Potential uses of synthetic genetic circuits.

Genetic Circuits Design

Genetic circuits are complex systems of interacting genes that can be designed to perform specific functions, such as producing valuable chemicals or sensing environmental signals. The field of synthetic biology has enabled the creation of increasingly sophisticated genetic circuits, which has great potential for various industries. CD Biosynsis provides a wide range of services for genetic circuits design in this field.

Figure 1. Biochemical metrics for assessing designed sequence quality across test cases and design methods. (Anand N, et al., 2020)

Protein Sequence Design and Optimization

CD Biosynsis provides professional sequence design and optimization services for synthetic biology researchers around the world to facilitate their cutting-edge research.

Fig 1. Sustainable production of chemicals by combining industrial process optimization and genetic engineering approaches.

Chemical production Metabolic pathways Engineering

CD Biosynsis is committed to providing high-quality services to our clients. Our specialized synthetic biology platform specializes in providing tools, libraries of gene parts for metabolic pathways, and bioengineering for chemical production, as well as designing and optimizing metabolic pathways for maximum efficiency.

Major aspects of the de novo protein design.

De Novo Protein Design

De novo protein design is revolutionizing synthetic biology by enabling the creation of tailor-made proteins with unprecedented functions. Our services empower industries and researchers to harness the potential of these engineered biomolecules for diverse applications, from therapeutics to materials science.

Assay design for high-throughput screening.

Strain Hight-throughput screening

Our high-throughput screening services are dedicated to assisting researchers and organizations in achieving their scientific goals efficiently and effectively, leveraging state-of-the-art technology and expertise to accelerate progress in diverse fields of study.

Automation Screening

Strain Automation screening

Automation screening is a pivotal technology in synthetic biology, revolutionizing research across various domains. By harnessing the power of automation, CD Biosynsis' services contribute to the advancement of life sciences, enabling rapid, systematic, and high-throughput analysis that was previously unattainable through manual methods.

Concept of the systems metabolic engineering framework.

Bacterial Strain Modification

Bacterial strain modification is a powerful tool in synthetic biology, allowing for the manipulation of bacterial characteristics to improve their efficiency in various applications.

Fig 1. Direct combinatorial pathway optimization.

Biosynthesis Pathway design and optimization services

CD Biosynsis has expertise in pathway engineering, computational tools, and collaborative approaches that are well suited to meet the diverse needs of our clients in synthetic biotechnology.

Oligo Synthesis

Oligo Synthesis Service

Oligo Synthesis is the process of creating short sequences of nucleic acids, known as oligonucleotides. These oligonucleotides are widely used in various research applications, such as PCR, gene synthesis, and DNA sequencing. They play a crucial role in molecular biology and biotechnology.

Gene Knockout Services

Gene knockout services offer a powerful approach in molecular biology for understanding gene function and creating disease models. This advanced technique involves the complete inactivation or "knocking out" of a specific gene, allowing researchers to study the resulting phenotypic changes and gain insights into the gene's role in biological processes. Gene knockout is an essential tool for genetic research, enabling the dissection of gene pathways and the development of therapeutic strategies.

Potential uses of synthetic genetic circuits.

Genetic Circuit Design and Engineering

Genetic circuits are complex systems of interacting genes that can be designed to perform specific functions, such as producing valuable chemicals or sensing environmental signals. The field of synthetic biology has enabled the creation of increasingly sophisticated genetic circuits, which has great potential for various industries. CD Biosynsis provides a wide range of services for genetic circuits design in this field.

In silico screening of peptide from edible peptide database.

Strain In silico screening

In silico screening is revolutionizing synthetic biology by offering a powerful and cost-effective approach to biological research and drug discovery. Our comprehensive services, coupled with cutting-edge technology and expert guidance, position us at the forefront of this transformative field. Unlock the full potential of your research with our In Silico Screening Services.

Genetic Engineering and Gene Editing

Genetic Engineering and Gene Editing

Genetic Engineering is the manipulation of an organism's genetic material to introduce desired characteristics or traits. It involves altering the DNA sequence of an organism to achieve specific outcomes, such as increased crop yield or improved disease resistance.

Service process flow for site-directed mutagenesis at CD Biosynsis.

Site-Directed Mutagenesis

Site-directed mutagenesis is a very useful in vitro technique to create specific, targeted mutations in a known DNA sequence. It can efficiently change the characterization of target proteins. Site-directed mutagenesis can be used as a precision tool to enable synthetic biology. It plays an essential role in a variety of synthetic biology research, such as the study of gene regulatory elements, protein structure and functions, enzyme active sites and novel proteins.

A schematic diagram representing typical bottlenecks in the secretory pathway of heterologous proteins in yeast.

Yeast Strain Modification

Yeast strain modification is a process of altering the genetic material of yeast cells to introduce new traits or improve existing ones. This process has been widely used in various industries such as food and beverage, pharmaceuticals, and biofuels. Yeast strain modification involves the use of genetic engineering techniques to introduce genes or delete genes that control specific traits, such as fermentation rate, sugar metabolism, or stress resistance.

The primary lutein biosynthetic pathway.

Biosynthesis Screen assay and characterization

CD Biosynsis provides a comprehensive suite of tools to accelerate your synthetic biology research. By harnessing the power of high-throughput screening and in-depth characterization, we empower researchers and innovators to unlock the full potential of biological systems for a wide range of applications, from drug discovery to biotechnology advancements.

DNA Assembly and Cloning Services

DNA Assembly and Cloning Services

Through DNA assembly and cloning services, CD Biosynsis aims to provide researchers and innovators with streamlined access to complex genetic constructs, which will alleviate the technological challenges associated with DNA manipulation and enable researchers to focus on their core objectives, facilitating their breakthroughs in different fields.

Figure 1. Schematic representation of the differences and convergence between synthetic biology and metabolic engineering. (Pouvreau B, et al., 2018)

Plant Metabolic Engineering

CD Biosynsis provides comprehensive services for plant metabolic engineering to facilitate the cutting-edge research of synthetic biology researchers around the world.

Metabolic Pathway Synthesis and Optimization

Metabolic Engineering

Metabolic engineering and synthetic biology have combined to become one of the most promising fields. CD Biosynsis provides a comprehensive portfolio of metabolic services for the build phase of the Design-Build-Test-Learn (DBTL) cycle to help synthetic biology researchers around the world facilitate their cutting-edge research. We provide powerful tools and tailored solutions to help our customers overcome the challenges in synthetic biology and bring diverse creative ideas to fruition.

Figure 1. Comparison of various commercial recombinant protein expression systems. (Raskin I, et al., 2002)

Cell-Based Protein Expression

CD Biosynsis has a team of experienced and highly skilled scientists to provide synthetic biology researchers around the world with high-quality cell-based protein expression services to facilitate their cutting-edge research.

Figure 1. Cloned libraries – RNAi and peptide libraries. (Murgha Y E., 2012)

Synthetic DNA Library Construction

CD Biosynsis provides high-quality synthetic DNA library construction services for synthetic biology researchers around the world to facilitate their cutting-edge research. Our strong expertise coupled with advanced techniques can help bring diverse synthetic applications and creative ideas to fruition.

Various types of epigenetic modifications under stress conditions.

Plant Strain Modification

Plant strain modification, also known as plant breeding or genetic engineering, involves the deliberate alteration of a plant's genetic makeup to achieve specific desired traits. This process aims to improve crop yield, quality, resistance to pests and diseases, and tolerance to various environmental conditions. Plant strain modification is a powerful tool in plant biotechnology that enables the manipulation of plant traits to improve crop yields, enhance disease resistance, and increase nutritional value.

Figure 1. Schematic diagram of the backbone building process at CD Biosynsis.

Protein Backbone Building

CD Biosynsis provides professional backbone building services for synthetic biology researchers around the world to facilitate their cutting-edge research. Our aim is to obtain protein backbone configurations that satisfy a set of predefined requirements by the customers.

Integrated approaches in metabolic engineering(A Lechner, et al.,2016)

Metabolic Engineering and Pathway Design

Metabolic Engineering and Pathway Design is a specialized offering provided by our company. It involves the modification of metabolic pathways in microorganisms, particularly E. coli, to optimize the production of desired chemicals. Through targeted genetic modifications, we can enhance the metabolic capabilities of these organisms, enabling them to efficiently synthesize valuable chemicals. Our team of experts utilizes state-of-the-art techniques and tools to design and engineer metabolic pathways that are tailored to meet your specific requirements.

Figure 1. A snapshot of synthetic biology tools used for modification of microbial enzymes. (Shukla P, 2019)

Enzyme Engineering

CD Biosynsis provides professional enzyme engineering services to facilitate the cutting-edge research of synthetic biology researchers around the world.

Strain Development Services

Strain Development Services

Strain Development Services involve the modification, optimization, and customization of microorganisms, cells, or other biological entities for specific applications. This field plays a crucial role in various industries, including biotechnology, pharmaceuticals, agriculture, and environmental science. The goal is to engineer or develop strains with desired characteristics, such as increased productivity, enhanced functionality, improved resistance to environmental factors, or the expression of specific traits.

An overview of the key approaches towards developing a microorganism platform for improved viability.

Strain Engineering Services

CD Biosynsis has been at the forefront of strain engineering research and development for years. The team of experts has dedicated to understanding the complex interactions between microorganisms and their environments, and have developed innovative strategies to optimize their performance in various applications.

Protein Structure Determination

Protein Structure Determination

CD Biosynsis provides comprehensive protein characterization services for synthetic biology researchers around the world to facilitate their cutting-edge research.

Protein Analysis Services

Protein Analysis Services refers to a series of experimental technologies and services used to characterize proteins. These services cover everything from the detection, purification, and identification of proteins to detailed analysis of their structure and function. These services are widely used in fields such as biomedical research, drug development and industrial production.

Diagram of modular plasmid design.

Plasmid Design and Transformation

Plasmids are small, self-replicating circular DNA molecules that play a crucial role in synthetic biology. They are used to introduce genes of interest into host cells, allowing for the manipulation and engineering of biological systems.

Gene Circuit Design Services

Gene circuit design services provide an innovative approach to synthetic biology, enabling the creation of custom genetic circuits that can regulate cellular behavior, control gene expression, and perform complex biological functions. These services offer comprehensive solutions for designing, constructing, and validating gene circuits tailored to your specific research or biotechnological needs. By leveraging advanced tools and expertise in genetic engineering, we help you develop sophisticated gene circuits that can revolutionize research and therapeutic applications.

Metabolic Pathway Optimization in Strains

Strain Pathway Optimization

Synthetic biology is a rapidly growing field that has revolutionized the way we think about the production of biological compounds. One of the key challenges in synthetic biology is the optimization of metabolic pathways in strains to produce high-value compounds. Metabolic pathway optimization involves the design, engineering, and optimization of the enzymatic and regulatory processes that control the production of specific compounds in microbial cells. However, the complexity of cellular metabolism and the limited understanding of the underlying mechanisms make the optimization of metabolic pathways a tedious and time-consuming process. Therefore, new approaches are required to accelerate the pathway optimization process and improve the efficiency and accuracy of the metabolic engineering process.

CRISPR‒Cas9-based genome-editing approaches.

Genetic Engineering

Genetic engineering has revolutionized various industries, including healthcare, agriculture, and biomanufacturing. The field of synthetic biology, which involves the design and construction of new biological systems or redesigning existing ones, has further expanded the possibilities of genetic engineering. Genetic engineering has been a rapidly evolving field over the past few decades, with the development of new technologies and techniques that have made it possible to manipulate genes with unprecedented precision and efficiency. The potential of genetic engineering to revolutionize various industries has been well-established, but it also raises ethical and regulatory challenges that need to be addressed.

Graphical abstract of evaluation of strains.

Strain Performance Evaluation

In the rapidly advancing field of synthetic biology, the performance evaluation of strains plays a pivotal role in optimizing biological systems for various applications. Synthetic biology combines principles from biology, engineering, and computer science to design and construct new biological parts, devices, and systems with enhanced functionalities.

Graphical abstract of UV-Vis process monitoring.

Strain Fermentation Monitoring

Fermentation is a fundamental biological process that has been used for centuries to produce a wide range of products, including food, beverages, pharmaceuticals, and biofuels. Traditional methods of fermentation monitoring involve time-consuming and costly methods such as manual sampling and offline analysis. However, monitoring the fermentation process in real-time can be challenging due to the complex interactions between microorganisms, substrates, and environmental factors.

Figure 1. Large-scale reengineering of organisms. (Standage-Beier K & Wang X, 2017)

Genome Reprogramming Service

CD Biosynsis provides custom genome reprogramming services for synthetic biology researchers around the world to facilitate their cutting-edge research. Our strong expertise coupled with advanced techniques can help bring diverse synthetic applications and creative ideas to fruition.

Time-resolved extracellular metabolite concentrations (mmol/l) of COL and HG001 were visualized with MeV as color coded chart.

Strain Metabolic Throughout Analysis

Synthetic biology involves engineering biological systems through the design and construction of new biological parts, devices, and systems, or the modification of existing ones. One of the key challenges in synthetic biology is to create efficient and reliable metabolic pathways in microbial strains.

(Z Wu, et al.,2021)

Protein sequence design and optimization

Protein sequence design and optimization is a scientific process of creating or modifying protein sequences to achieve desired functional properties or enhance protein stability. It involves utilizing computational algorithms and experimental techniques to design novel protein sequences with specific structural and functional characteristics.

Novel CRISPR System

Novel CRISPR Gene Editing Service

In a world where scientific breakthroughs hinge on innovative tools, our novel CRISPR system stands at the forefront of genetic engineering innovation. With its precision, versatility, and diverse applications, it empowers researchers and industry leaders to push the boundaries of what's possible in the realm of synthetic biology.

Fig 1. Unbiased libraries in protein directed evolution.

Protein library design

CD Biosynsis's synthetic biology platform is dedicated to providing high-quality protein library design services to help biologists around the world design protein variant collections to support innovation in a variety of fields.

Components of precision engineering technology for microbial strain improvement.

Strain Improvement Service

Strain improvement is a critical aspect of synthetic biology that involves the targeted enhancement of microorganisms or cell lines for specific applications. It revolves around modifying the genetic, metabolic, and physiological characteristics of these organisms to optimize their performance in various industrial, medical, or research contexts.

The mechanisms of genome editing and DSB repair.

Strain Gene Editing

Synthetic biology is a rapidly growing field that has the potential to revolutionize many industries, including biotechnology, medicine, and agriculture. One of the key tools in synthetic biology is gene editing, which allows scientists to manipulate the DNA of organisms to create new functions, optimize metabolic pathways, and engineer new traits. In recent years, the development of new gene editing tools, such as CRISPR-Cas9, has made gene editing faster, cheaper, and more precise than ever before.

PCR Cloning

PCR Cloning Service

PCR Cloning is a powerful molecular biology technique that allows scientists to amplify a specific DNA sequence and insert it into a vector for further study or manipulation. This method has revolutionized the field of genetic engineering by enabling the creation of multiple copies of DNA fragments, which can be used for various applications in molecular biology and biotechnology.

Single-Cell Gene Editing Service

Single-cell gene editing represents a pinnacle of precision and specificity in molecular biology, offering an unparalleled approach to genetic research and therapeutic development. This advanced service involves the modification of genetic material within individual cells, allowing researchers to dissect gene function with exceptional accuracy, model intricate disease mechanisms, and develop tailored therapeutic strategies. The importance of this service is profound, as it provides critical insights into cellular heterogeneity and genetic variability, paving the way for innovative treatments and personalized medicine.

Protein Purification Service

Protein Purification Service is an important service in the field of biotechnology. It aims to extract and purify high-purity, biologically active proteins from organisms or protein-containing solutions through a series of complex experimental steps. This process usually includes multiple stages such as rough separation, preliminary purification and fine purification, using various technical means such as extraction, precipitation, centrifugation, chromatography, etc. to remove impurities while maintaining the biological activity of the protein.

(Y Cai, et al.,2020)

Base editing service

Base editing is an innovative and groundbreaking technology that allows for precise modifications to be made in the DNA of living organisms. It provides a unique approach to genetic engineering by enabling the direct conversion of one DNA base pair to another, without the need for introducing foreign genetic material. This targeted approach holds tremendous potential for a wide range of applications, including the treatment of genetic diseases, the enhancement of crop yields, and the advancement of scientific research.

Biosynthetic gene cluster and proposed biosynthetic pathway of 1.

Biosynthesis Gene Cluster

CD Biosynsis specializes in Biosynthesis of gene cluster services, offering cutting-edge solutions for synthetic biology applications. Our comprehensive services encompass gene cluster analysis, design, and optimization to drive innovation in biotechnology.

 Engineering protein, metabolism and artificial cell in the open cell-free system.

Biosynthesis Cell free system

CD Biosynsis harnesses the transformative power of the Cell-Free System to catalyze innovation across the biotech landscape. With an unwavering focus on precision, quality, and versatility, we empower researchers and biotech entities to explore new horizons and unlock the full potential of molecular biology. Join us on the journey to redefine the future of biotechnology through our pioneering Cell-Free System Services.

Schematic representation of cell-free methodology modification for the production of diverse proteins.

Protein Biosynthesis

CD Biosynsis specializes in cutting-edge protein biosynthesis services. Leveraging synthetic biology techniques, we deliver precise and efficient protein synthesis solutions tailored to your unique research and industrial needs.

Salmonella Genome Editing Service

Our Salmonella Genome Editing Service offers precise and efficient solutions for genetic modifications in Salmonella species, optimizing them for use in research, vaccine development. Utilizing state-of-the-art genome editing technologies such as CRISPR/Cas9, we provide comprehensive support from project design to final validation, ensuring your genome editing goals are achieved with high accuracy and efficiency.

Pseudomonas aeruginosa Genome Editing Service

Our Pseudomonas aeruginosa Genome Editing Service offers precise and efficient solutions for genetic modifications in Pseudomonas aeruginosa, optimizing them for use in research, industrial applications, and medical biotechnology. Utilizing state-of-the-art genome editing technologies such as CRISPR/Cas9, TALENs, and recombineering, we provide comprehensive support from project design to final validation, ensuring your genome editing goals are achieved with high accuracy and efficiency.

Schematic of insulin production and secretion.

Insulin Strain Engineering

Our specialized biotechnology services are dedicated to the precise biosynthesis of insulin, exclusively for scientific research purposes. Our commitment is to provide state-of-the-art technical solutions in the realm of insulin production, catering to the exacting needs of researchers and laboratories.

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