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Trusted by Leading Research & Pharma Institutions

Custom Membrane Protein Expression Services

From GPCRs to multi-pass transmembrane proteins, our specialized platforms deliver high-quality membrane proteins with native structure and authentic post-translational modifications. Trusted by leading pharmaceutical companies, biotechnology firms, and academic research institutions worldwide for structural biology, drug discovery, and diagnostic development applications.

Native Structure
Multiple Platforms
High Success Rate
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Trusted by leading research and pharmaceutical institutions

Harvard
Pfizer
MIT
Roche
Stanford
Novartis

Why Choose Us

200+ membrane proteins successfully delivered
Multiple expression platforms available
Expert consultation included
Comprehensive QC documentation

Mammalian Cells

HEK293, Expi293F for human proteins

Insect Cells

Sf9, High Five for complex proteins

Cell-Free Systems

High-throughput screening options

Success Rate
85%+
Overview Technology Specifications Workflow Applications FAQ
Service Overview

Expert Membrane Protein Expression Services

Membrane proteins represent over 60% of all drug targets yet remain among the most challenging proteins to express and purify. Our specialized platforms, combined with extensive technical expertise, overcome these obstacles.

Native Structure Preservation

Our specialized platforms maintain native protein structure throughout the expression and purification process. Proper lipid environment, post-translational modifications, and functional conformation are preserved for downstream applications.

  • Authentic post-translational modifications
  • Proper protein folding
  • Functional activity verification

Multiple Expression Platforms

We offer a comprehensive suite of expression systems optimized for different membrane protein types. From mammalian cells for human proteins to insect cells and cell-free systems, we match the right platform to your project.

  • Mammalian cells (HEK293, Expi293F)
  • Insect cells (Sf9, High Five)
  • Cell-free expression systems

Structural Biology

Cryo-EM, X-ray crystallography, and NMR studies require high-quality, homogeneous samples.

Drug Discovery

High-throughput screening and binding assays for therapeutic development.

Diagnostic Development

Receptor proteins for immunoassays and diagnostic platform development.

Ready to Express Your Membrane Protein?

Get expert consultation and customized quote for your project.

Technology Platform

Advanced Expression Technologies

Multiple platforms optimized for diverse membrane protein types and applications.

Mammalian Cell Platforms

HEK293 and Expi293F cells provide authentic human-like processing including proper glycosylation, disulfide bond formation, and lipid modifications essential for functional membrane proteins.

HEK293 Expi293F

Insect Cell Systems

Sf9 and High Five cells offer cost-effective production with proper eukaryotic processing. Ideal for structural studies requiring larger quantities of protein.

Sf9 High Five

Cell-Free Expression

High-throughput screening of expression conditions using cell-free systems. Direct incorporation of labeled amino acids and rapid optimization.

HTP Screening Labeled Proteins

Quality Control

SDS-PAGE Purity assessment
SEC-MALS Aggregation analysis
MS Mass spectrometry verification
Activity Functional assay when applicable

Purification Options

Affinity His-tag, Flag-tag, Strep-tag
Detergent DDM, LMNG, GDN screening
Nanodiscs MSP-based stabilization
Lipids Native-like environment
Specifications

Flexible Options for Diverse Needs

Comprehensive specifications to meet your research requirements.

Parameter Mammalian Cells Insect Cells Cell-Free
Protein Types GPCRs, Channels, Transporters Multi-pass, Large complexes Screening, Labeled proteins
Scale Range 1-50 mg 5-100 mg 0.1-5 mg
Turnaround Time 4-8 weeks 3-6 weeks 1-3 weeks
Glycosylation Human-like Complex-type None
Purification Affinity, SEC, IMAC Affinity, SEC Affinity, His-tag
QC Analysis SDS-PAGE, SEC-MALS, MS SDS-PAGE, SEC-MALS SDS-PAGE, MS
Workflow

Streamlined Process from Design to Delivery

Our proven workflow ensures quality and efficiency at every stage.

1

Consultation

Project assessment and strategy design

2

Expression

Optimized platform selection

3

Purification

Multi-step purification

4

QC

Comprehensive quality analysis

5

Delivery

Secure delivery with COA

Applications

Diverse Applications Across Research

Our membrane protein expression services support research in multiple fields.

Structural Biology

High-quality, homogeneous membrane protein samples for Cryo-EM, X-ray crystallography, and NMR studies. Our optimized expression and purification protocols deliver samples suitable for high-resolution structural determination.

  • Cryo-EM sample preparation
  • X-ray crystallography
  • NMR spectroscopy
  • Nanodisc incorporation
200+
Membrane proteins delivered

Therapeutic Development

Functional membrane proteins for drug discovery, high-throughput screening, and therapeutic antibody development. Our mammalian expression systems ensure proper folding and post-translational modifications.

  • HTS assay development
  • Binding studies
  • Lead optimization
  • Biophysical characterization
85%+
Success rate

Diagnostic Development

Receptor proteins and antigens for diagnostic assay development. From ELISA components to lateral flow assays, we provide the proteins you need for accurate and reliable diagnostics.

  • Immunoassay development
  • Lateral flow assays
  • Biosensor development
  • Quality control reagents
18+
Years experience
Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The membrane protein samples exceeded our expectations. The Cryo-EM data showed excellent particle quality. The team's expertise in membrane protein biology was evident throughout the project."

R
Principal Scientist
Structural Biology Company

"Their expertise in GPCR expression was invaluable. They recommended the optimal expression system and detergent conditions. The protein worked perfectly in our HTS assay."

P
Research Director
Pharmaceutical Company

"Excellent service from start to finish. The consultation phase helped us design the best experimental approach. Deliverables were on time with comprehensive QC documentation."

L
Lead Researcher
Academic Research Institution
Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

287 Citations

Expression of eukaryotic membrane proteins in eukaryotic and prokaryotic hosts

Kesidis A, Depping P, Lode A, Vaitsopoulou A, Bill RM, Goddard AD, Rothnie AJ. Methods. 2020.

Comprehensive comparison of membrane protein expression systems including E. coli, yeast, insect cells, and mammalian cells.

View DOI
78 Citations

Database Study on the Expression and Purification of Membrane Proteins

Chen-Yan Zhang, Shi-Qi Zhao, Shi-Long Zhang, et al. Protein and Peptide Letters. 2021.

Systematic analysis of membrane protein expression systems based on PDB database, recommending HEK293 for human proteins.

View DOI
89 Citations

High-throughput cell-free screening of eukaryotic membrane protein expression in lipidic mimetics

Lyons JA, Stefanić MP, Wallbrecht N, Chen Y, et al. Scientific Reports. 2021.

Cell-free expression platform for high-throughput screening of 35 eukaryotic membrane proteins.

View DOI
52 Citations

Screening of Membrane Protein Production by Comparison of Transient Expression in Insect and Mammalian Cells

Kaipa JM, Krasnoselska GO, Owens RJ, van den Heuvel J. Biomolecules. 2023.

Comparison of eukaryotic membrane protein expression in insect and mammalian cells for structural studies.

View DOI
64 Citations

Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells

Krasnoselska GO, Dumoux M, Gamage N, et al. Methods in Molecular Biology. 2021.

Detailed protocols for transient transfection and expression of membrane proteins in Expi293F cells.

View DOI
FAQ

Frequently Asked Questions

Find answers to common questions about our service.

The best expression system depends on your protein type and downstream application. Mammalian cells (HEK293, Expi293F) are recommended for human membrane proteins requiring authentic post-translational modifications. Insect cells (Sf9, High Five) offer higher yields for complex proteins. Cell-free systems are ideal for screening conditions and producing labeled proteins. Our consultation team will help you select the optimal platform.
We offer multiple purification strategies including affinity chromatography (His-tag, Flag-tag, Strep-tag), detergent-based purification, and alternative stabilization methods. Detergent screening is available to identify the optimal conditions for your protein stability. We also offer nanodisc incorporation for proteins requiring a native-like lipid environment.
Standard QC includes SDS-PAGE analysis for purity, SEC-MALS for aggregation assessment, and mass spectrometry for sequence verification. Additional analyses such as thermal stability assays, binding assays, and functional activity testing are available upon request. All deliverables include a Certificate of Analysis.
Turnaround times vary by expression system and project complexity. Cell-free screening typically takes 1-3 weeks. Mammalian cell expression requires 4-8 weeks. Insect cell expression takes 3-6 weeks. Expedited services are available for urgent projects. Contact us for a detailed timeline based on your specific requirements.
Yes, we have extensive experience with challenging targets including multi-pass transmembrane proteins, G-protein coupled receptors, ion channels, and large protein complexes. Our platforms include specialized strategies such as codon optimization, signal peptide screening, and detergent formulation optimization to maximize expression success.
We offer flexible scale options from milligram to multigram quantities. Small-scale expression screening (1-5 mg) is ideal for initial characterization. Medium-scale production (5-50 mg) supports most structural biology and drug discovery applications. Large-scale production (100+ mg) is available for crystallography screening and therapeutic development.

Ready to Start Your Project?

Get a customized quote for your Custom Membrane Protein Expression Services project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

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