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Strain Engineering for Aspartic Acid

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As a customer-focused synthetic biology company, CD Biosynsis has a team of experienced scientists to help our customers develop synthetic biology tools and strategies to improve the production of aspartic acid and meet rising global market demand. We are committed to harnessing the power of synthetic biology to drive sustainable bioproduction of aspartic acid and towards scale-up production.

Advantages of Synthetic Biology-Driven Aspartic Acid Production

Aspartic acid or aspartate with the molecular formula C4H7NO4 is a nonessential amino acid used in protein biosynthesis. The successful discovery of aspartame and the development of poly(aspartic acid) have greatly contributed to accelerate the aspartic acid market growth. Therefore, it is important to develop innovative technologies that can improve the efficiency of aspartic acid production and reduce the cost. Enzymatic conversion in the form of simple enzyme-substrate interaction process or fermentation is currently the preferred method of choice for aspartic acid production compared to protein extraction and chemical synthesis. Rapid developments in synthetic biology have made it possible to produce aspartic acid and its derivatives from renewable raw material in microbial cell factories by biological methods, which will help to establish a sustainable and green approach as a new paradigm for the industrial production of aspartic acid in a cost-effective manner.

Figure 1. L-Aspartic acid formation, from fumaric acid and maleic acid. (Yukawa H, et al., 2009)Figure 1. L-Aspartic acid formation, from fumaric acid and maleic acid. (Yukawa H, et al., 2009)

What We Provide

Leveraging our powerful synthetic biology platform, CD Biosynsis can provide our customers with custom synthetic biology services to help them develop effective strategies to achieve efficient biosynthesis of aspartic acid.

Featured Services

  • Development of metabolic engineering strategies for efficient microbial production of aspartic acid from biomass.
  • Design and construction of novel biosynthetic pathways to maximize the stoichiometric yield of aspartic acid through the shortest pathway.
  • Development of effective fine-tuning strategies to direct metabolic flux toward aspartic acid biosynthesis rather than by-product formation.
  • Identification and overexpression of key enzymes in host strains for the improvement of aspartic acid productivity.


  • Aspartic acid-producing microorganisms.
  • High-quality L-aspartic acid, D-aspartic acid, and their derivatives such as polyaspartic acid.

How We Can Help

CD Biosynsis has been working on developing synthetic biology tools and strategies for engineering microorganisms to improve microbial production of aspartic acid. Our focus is on the biological process of aspartic acid production from fumaric acid and maleic acid. To meet growing demand for bio-based aspartic acid and their derivatives, our scientists are committed to helping our customers develop genetically modified strains for high-yield and cost-effective production of aspartic acid and optimize the production process.

Discovery and Selection of Microbial Strains for Aspartic Acid Production

We can help discover microbial strains with high aspartase and meleate isomerase activity and select high-aspartic acid-producing strains. Our scientists have experience in the optimization of culture conditions for aspartic acid production in different microbial strains. Cell membrane permeabilization is also an important factor that we consider in the discovery and selection of aspartic acid-producing strains. We have built a collection of aspartic acid-producing microorganisms as follows. If you are interested in other microorganisms, please fill out the online inquiry form and tell us more about your project.

Pseudomonas fluorescens Pseudomonas aeroginosa Pseudomonas dacuntae Pseudomonas trifolli
Bacillus subtilis Bacillus megatherium Bacillus stearothermophilus Proteus vulgaris
Escherichia coli Candida hydrocarbofumarica Brevibacterium flavum Cornybacterium glutamacium

Enzyme Discovery and Engineering for Aspartic Acid Production

We aim to develop effective enzyme engineering strategies to improve activity, stability, and selectivity of key enzymes such as aspartase and maleate isomerase in aspartic acid production. We are also committed to discovering novel highly active and stable enzyme, and develop new pathways for efficient aspartic acid production. Our scientists can help select candidate microorganisms and develop genetically engineered strains that overexpress genes encoding highly active enzymes to enhance aspartic acid production and eliminate byproducts such as L-malic acid.

Applications of Aspartic Acid

CD Biosynsis offers our expertise in synthetic biology to meet the increased demand for aspartic acid in various industries including food, feed, beverage, cosmetic, agriculture, and pharmaceutical industries.

  • Used as artificial sweetener in the form of aspartame.
  • Used as a flour coating in bakery products.
  • Used as nutritional supplement and flavor enhancer in food and beverages.
  • Used as nutritional additives in fertilizers.
  • Used in the manufacturing of organic chemicals.
  • Used as a raw material for the manufacture of synthetic resin and environmental protection materials.
  • Used as an intermediate in pharmaceuticals.
  • Used as biochemical reagents and culture agents.
  • Used as an active ingredient in anti-aging cosmetics and nutritional additive in cosmetics.
  • Used as a component of ammonia detoxifier, fatigue eliminating agent, and liver function promoters in medicine.

Want to Learn More?

As a rapidly growing synthetic biology company, CD Biosynsis is committed to helping our customers meet the growing and evolving demand for amino acid production. All of our deliverables will undergo a rigorous quality testing process to ensure the quality and reliability and can be delivered on time. If you are interested in our services or have any further questions, please do not hesitate to contact us.


  1. Yukawa H, et al. L-aspartic acid, production processes. Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, 2009: 1-3.
  2. Appleton H, Rosentrater K A. Sweet dreams (Are made of this): A review and perspectives on aspartic acid production. Fermentation, 2021, 7(2): 49.

Please note that all services are for research use only. Not intended for any clinical use.

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