Our Protein Expression Service offers a comprehensive solution for researchers and organizations seeking high-quality protein production. Protein expression is a vital process in the field of molecular biology, enabling the production of specific proteins for various research purposes. At our company, we have established ourselves as experts in protein expression, utilizing advanced techniques and state-of-the-art facilities to efficiently express and purify proteins of interest.
Overview of processing pathways and major N-glycans produced by insect and mammalian cell systems (TA Kost, et al.,2005)
Services Offered
Service Process
FAQs
Services Offered
We understand that different research projects have unique requirements, which is why we offer a range of protein expression systems tailored to meet diverse needs. Our services cover expression in bacterial, yeast, insect, and mammalian cell systems, providing flexibility and versatility in protein production. Whether you require proteins for structural biology, enzymology, drug screening, or other applications, we have the expertise to deliver reliable and high-yield protein expression solutions.
| Recombinant Protein Expression Type |
Applications |
Description |
| Bacterial Expression Systems |
- Biopharmaceuticals, industrial enzymes, protein production for research. |
- Description: Utilizes bacterial cells, such as E. coli, for the expression of recombinant proteins. Bacterial systems are often chosen for their simplicity, rapid growth, and cost-effectiveness. However, they may lack post-translational modifications present in eukaryotic cells. |
| Yeast Expression Systems |
- Production of eukaryotic proteins, industrial enzymes, vaccine antigens. |
- Description: Utilizes yeast cells, such as Saccharomyces cerevisiae or Pichia pastoris, for the expression of recombinant proteins. Yeast systems offer eukaryotic protein folding and post-translational modifications, making them suitable for certain applications requiring native-like protein structures. |
| Mammalian Expression Systems |
- Production of therapeutic proteins, antibodies, and proteins requiring complex post-translational modifications. |
- Description: Utilizes mammalian cells, often human or CHO (Chinese Hamster Ovary) cells, for the expression of recombinant proteins. Mammalian systems provide native-like post-translational modifications, making them suitable for the production of therapeutic proteins and complex biologics. |
| Insect Cell Expression Systems |
- Production of eukaryotic proteins, vaccine antigens, virus-like particles. |
- Description: Utilizes insect cells, often derived from baculoviruses, for the expression of recombinant proteins. Insect cell systems offer advantages of eukaryotic protein folding and post-translational modifications, making them suitable for certain applications, especially when mammalian systems are not feasible. |
Service Process
Our Protein Expression Service follows a streamlined process to ensure efficient and successful protein production. We understand that every project is unique, and we work closely with our clients to meet their specific requirements. Here is a breakdown of our service process:
- Consultation: We begin by understanding your specific protein expression needs. During this phase, we discuss factors such as the target protein, desired yield, and any specific purification needs. Our team of experts will guide you through the process and provide valuable insights to optimize your protein expression project.
- Design and Optimization: Based on the information gathered during the consultation phase, our team of experienced scientists designs a customized expression strategy. We take into account factors such as the characteristics of the target protein, the expression system to be used, and the desired protein yield. Through rigorous optimization, we aim to maximize protein yield and quality.
- Expression and Purification: Once the expression strategy is finalized, we proceed with the expression and purification of the protein of interest. We utilize advanced techniques and state-of-the-art equipment to ensure efficient protein expression and purification. Our team is experienced in employing chromatography and other purification methods to achieve high purity levels.
- Quality Control: Quality control is a crucial aspect of our Protein Expression Service. We perform rigorous assessments to ensure that the delivered protein meets your specifications. This includes protein quantification, SDS-PAGE analysis, and endotoxin testing. By adhering to stringent quality control measures, we guarantee the reliability and consistency of our protein expression services.
- Delivery: After successfully expressing and purifying the protein, we carefully package and deliver it to you. We understand the importance of timely delivery, and we strive to provide quick turnaround times. Along with the protein, we provide a detailed report summarizing the expression and purification process, ensuring transparency and traceability.
We are dedicated to delivering reliable and high-quality Protein Expression Services. With our expertise and commitment to scientific excellence, we are here to support you in achieving your research goals. Contact us today to discuss your project and how we can assist you in your protein expression needs.
FAQs
Q: How long does the protein expression process typically take?
A: The timeline for protein expression can vary depending on several factors, including the complexity of the protein and the expression system used. During the consultation phase, we will provide you with an estimated timeline based on the specific requirements of your project.
Q: Can you handle difficult-to-express proteins?
A: Yes, we have extensive experience in expressing a wide range of proteins, including those that are considered challenging. Our team of experts employs various strategies and optimization techniques to enhance the expression of difficult proteins. We are committed to finding innovative solutions to overcome expression challenges and deliver successful results.
Q: Do you offer protein characterization services?
A: While our primary focus is on protein expression and purification, we understand the importance of protein characterization in many research projects. Therefore, we also provide additional services, including protein characterization. Please let us know your specific requirements, and we will be happy to discuss the available options and provide you with comprehensive solutions.
Q: What are the payment options for your Protein Expression Service?
A: We offer flexible payment options to accommodate the needs of our clients. We accept various payment methods, including bank transfers, credit cards, and electronic payment platforms. Our team will work with you to determine the most convenient payment option for your project.
Q: Can you assist with scale-up protein production?
A: Yes, we have the capabilities to assist with scale-up protein production. Whether you require small-scale production for research purposes or large-scale production for commercial applications, we can tailor our services to meet your specific needs. Contact us to discuss your scale-up requirements and we will provide you with customized solutions.
Q: Is confidentiality ensured for my protein expression project?
A: Absolutely. We prioritize confidentiality and take measures to ensure the security of your project. Our team adheres to strict confidentiality agreements, and access to your project information is limited to authorized personnel. We are committed to safeguarding your intellectual property and maintaining the privacy of your research.
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