CD Biosynsis is dedicated to the development of dextran-producing bacteria and dextran sucrase-producing bacteria using genetic engineering and enzyme engineering technologies to promote the industrial production of dextran based on synthetic biology.
Dextran is a polymer formed by dehydration of several glucose, which is a mucus substance secreted by the mesenteric bacterium and is usually produced by microbial fermentation, which produces products with different molecular weights depending on the fermentation process parameters. The main methods currently used to obtain dextran are enzymatic synthesis and direct fermentation of the strain. It has been widely used in many fields such as medicine, food, and chromatographic analysis.
Figure 1. Predicted LAB pathways for sugar transport and catabolism with concomitant dextran production. (Besrour-Aouam N, et al., 2021)
We are able to use genetic engineering to clone and express the gene for dextran sucrase, and to mine for mutated genes related to enzyme function and recombine them homologously with the chromosomal DNA of existing engineered bacteria, thereby screening for engineered bacteria containing high-performance dextran sucrase.
Based on the catalytic mechanism of dextran sucrase and the conformational relationship of dextran, we synthesized recombinant dextran sucrase using enzyme engineering technology.
Development of Genetically Engineered Strains to Produce Dextran
We use molecular biology and genetic engineering techniques to analyze the regulation of sucrose metabolic pathway-specific regulators on dextran biosynthesis, mine the dextran synthesis gene cluster, screen for dextran sucrase synthesis genes, and use transcriptomics techniques to identify regulatory genes that potentially affect dextran sucrase expression, thus helping our customers to build dextran high-yield strains and laying the foundation for industrial production of dextran.
Development of Genetically Engineered Strains to Produce Dextran Sucrosease
Dextran sucrase is a glucosyltransferase produced by the fermentation of Leuconostoc mesenteroides and Streptococcu, which uses sucrose as a substrate. Since dextran sucrase is an inducible enzyme, both the inducer and the enzyme substrate are sucrose, and the resulting high-viscosity glucan forms a complex with the enzyme and the cell, causing difficulties in the extraction and purification of the enzyme and quality control of the catalytic product. Therefore, we are able to obtain recombinant dextran sucrase using genetic engineering and synthesize dextran by in vitro enzymatic methods.
Regulation of Dextran Enzymatic Synthesis
The molecular mass and the distribution of branched chains of dextran have a great influence on its properties. Therefore, the regulation of multiple parameters is important for the synthesis of dextran during the enzymatic synthesis of dextran. We are able to provide services for the regulation of parameters such as substrate concentration, enzyme amount, reaction temperature, and pH to help our customers establish the optimal parameters to optimize the synthesis process of dextran.
CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive dextran production and meet the needs of customers in a variety of industries.
CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in dextran production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.