CD Biosynsis

Engineered polyketide synthase with applied codon optimization strategies and targeted heterologous hosts

Codon Optimization for Different Expression Systems: Key Points and Case Studies

1 month ago1 month ago019 mins

1. Introduction to Codon Optimization Codon optimization represents an influential molecular biology method that modifies a gene’s nucleotide sequence to enhance protein production in a target organism. Scientists achieve improved translation efficiency and protein yield while preserving functional integrity when they match the gene’s codon usage to the host’s translational system. Achieving high expression levels…

Comparison of two alternative strategies for coupling phenotype and genotype in vitro

mRNA Display vs. Ribosome Display: Choosing the Right Technology for Your Research

1 month ago1 month ago018 mins

The protein engineering and drug discovery domains have undergone transformative changes due to mRNA display and ribosome display technologies which allow scientists to study protein functionality and evolve enzymes while identifying novel therapeutic agents. These techniques allow for the examination of extensive protein and peptide libraries although their operational principles and practical uses show significant…

Selection and evolution of proteins by mRNA display

Building Custom Libraries for mRNA Display: Strategies and Innovations

1 month ago1 month ago016 mins

The field of protein engineering and drug discovery now benefits from mRNA display technology as an effective means to identify proteins and antibodies with high affinity alongside peptides. Custom mRNA display libraries represent the fundamental element of this technology because they enable the identification of functional molecules. This blog post examines the techniques and innovative…

mRNA Display: Revolutionizing Drug Discovery Through In Vitro

1 month ago1 month ago021 mins

mRNA Display: Revolutionizing Drug Discovery Through In Vitro Evolution Introduction The identification of new drugs traditionally depended on examining natural substances or creating small molecular structures that interact with proteins. Traditional methods find many disease-related proteins like protein-protein interaction elements to be “undruggable.” Through mRNA display technology, scientists can now conduct in vitro evolution to produce…

Insights from Literature: Mannheimia succiniciproducens metabolic engineering for succinic acid production

5 months ago08 mins

Succinic acid is a 4-carbon dicarboxylic acid. Not only a major intermediate in the TCA, it is also a major raw material in food, pharmaceutical and chemical production. Since the market for sustainable development is increasing, renewable energy for microbial fermentation of succinic acid is gaining traction in the industry. But old-school fermentation tends to be coupled with…

Improved n-Butanol Production from Clostridium cellulovorans

Insights from Literature: Integrated metabolic and evolutionary engineering to help green production of n-butanol

5 months ago5 months ago010 mins

Driven by the global fossil fuel crisis and environmental issues, the development of biotechnology-based renewable fuels and chemicals has become a research hotspot. As an ideal petroleum alternative, n-butanol has attracted attention due to its superior fuel properties (high energy density, low volatility) and wide range of industrial uses. However, traditional butanol production relies on…

Biosynthetic pathways of L-malic acid in eukaryotes

Insights from Literature: L-Malate Biosynthesis and Metabolic Engineering Strategies

5 months ago05 mins

L-maleic acid is a four carbon dicarboxylic acid found in food, chemical and pharmaceutical industries. L-malic acid is an intermediate in the tricarboxylic acid cycle (TCA), one of the 12 most promising biobased platform chemicals – according to the US Department of Energy. Yet industrial production today mainly depends on chemical production of petrochemical raw materials and…

Application of CRISPR-Cas9 technology in metabolic engineering

6 months ago6 months ago023 mins

Introduction A brief introduction to CRISPR-Cas9 technology CRISPR-Cas9 technology is a revolutionary gene editing tool based on the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system found in bacteria and archaea. This system originally existed as a defense mechanism against phage infection. CRISPR-Cas9 technology uses Cas9 protein and guide RNA (gRNA) to identify and cut…

CRISPR/Cas9 gRNA library screening of functional genes

8 months ago8 months ago010 mins

CRlSPR/Cas9 is an acquired immune system used by bacteria and paleontological bacteria to resist the invasion of genetic material such as foreign plasmids or bacteriophages. The CRlSPR/Cas9 technology was used to establish a mammalian genome-wide mutation library or a gene mutant library related to a certain type of function, and the genes related to the…

Timeline of major events in the development of CRISPR Cas technology and representative Cas9 variants

Application of CRISPR/Cas9 gene editing technology

8 months ago8 months ago020 mins

Gene editing is an emerging genetic engineering technology that can modify specific target genes in the genome of organisms. Gene editing can efficiently perform site-directed genome editing, so it has shown great application prospects in gene research, gene therapy and gene breeding. To date, CRISPR/Cas9 has been widely used in the scientific community as an…

The world’s first AI designed and open-source CRISPR gene editor has successfully edited human DNA

Frontier | FDA approved new gene editing therapies for the treatment of OTC deficiency in clinical practice; LSTA1, a new drug for the treatment of osteosarcoma, has been recognized as an orphan drug

Gene editing therapy: Is Base editing and Prime editing technology the future?

Avantium stops its bio based polyol business and focuses on FDCA and PEF!

EU funded project to develop 24 bio based products using enzymes and strains from olive leaves

Ginkgo announces Q3 financial report: $55 million recorded, establishing new partnerships with Pfizer and Google

For the first time, a brand new enzyme was designed from scratch using AI, which can achieve ultra-high performance and unlimited quantity

High screening of non classical amino acid enzyme activity through the ability of coupling essential genes to resist termination codons

mRNA Display: Revolutionizing Drug Discovery Through In Vitro

Generative model enhances enzyme activity and stability

Construction of DNA Library~Fragmentation of DNA

Guidelines for metabolic flux analysis in metabolic engineering: methods, tools, and applications

Codon Optimization for Different Expression Systems: Key Points and Case Studies

mRNA Display vs. Ribosome Display: Choosing the Right Technology for Your Research

Building Custom Libraries for mRNA Display: Strategies and Innovations

mRNA Display: Revolutionizing Drug Discovery Through In Vitro

Insights from Literature: Mannheimia succiniciproducens metabolic engineering for succinic acid production

Insights from Literature: Integrated metabolic and evolutionary engineering to help green production of n-butanol

Insights from Literature: L-Malate Biosynthesis and Metabolic Engineering Strategies

Application of CRISPR-Cas9 technology in metabolic engineering

CRISPR/Cas9 gRNA library screening of functional genes

Application of CRISPR/Cas9 gene editing technology

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Artificial intelligence and synthetic biology are not bad omens

Targeted metabolism: the phenylalanine pathway metabolites you missed!

Glutathione, an antioxidant “artifact”

Comparative Analysis of Golden Gate Assembly and Gibson Assembly Techniques

Overview of Ergothioneine

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