Gene knockout is a powerful tool for studying gene function and has numerous applications in various fields, including basic research, biotechnology, and medicine. E. coli is an ideal organism for gene knockout studies due to its ease of manipulation, fast growth rate, and well-characterized genome. By disrupting specific genes in E. coli, researchers can investigate the role of those genes in various biological processes, including metabolism, protein synthesis, and stress response.
At CD Biosynsis, we offer a range of gene knockout services, including CRISPR-Cas9, homologous recombination, and RNAi, to help researchers unlock the full potential of synthetic biology.
Figure 1: Overview of CRISPR/Cas9 genome editing strategy in Escherichia coli. (König, E., et al. 2018)
At CD Biosynsis, we offer a wide range of gene knockout services for E. coli, including:
CRISPR-Cas9 is a revolutionary gene editing tool that allows for precise and efficient gene knockout in E. coli. Our experts use this technology to disrupt specific genes of interest, enabling researchers to study the resulting phenotype and its implications for bioproduct development.
Homologous recombination is another gene knockout method we offer, which involves the use of a selectable marker to replace the target gene with a new gene. This method allows for more precise control over the knockout event and can be used to study the effects of gene knockout on gene expression and protein function.
RNA interference (RNAi) is a gene silencing mechanism that can be used to knockdown specific genes in E. coli. By using RNAi, researchers can investigate the role of specific genes in various biological processes without completely disrupting them.
Gene knockout in E. coli offers several advantages, including:
Gene knockout in E. coli is a powerful tool for studying gene function and developing novel bioproducts. Our expertise and cost-effective services make us the ideal partner for any gene knockout project in E. coli. Contact us today to learn more about our services and how we can help you.
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.