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Biosynthesis Pathway design and optimization services

Biosynthesis Pathway design and optimization services

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Biosynthesis Pathway design and optimization services

CD Biosynsis has expertise in pathway engineering, computational tools, and collaborative approaches that are well suited to meet the diverse needs of our clients in synthetic biotechnology.

Introduction to Pathway Design and Optimization

Pathway design and optimization within the realm of synthetic biology refer to the procedure of designing and refining biological pathways, with the aim of attaining targeted functionalities or generating distinct compounds. In the context of synthetic biology, pathways are sequences of enzymatic reactions that orchestrate the conversion of initial substrates into ultimate products.

Pathway design and optimization hold significant importance in synthetic biology, as they facilitate the creation of novel and valuable biological systems tailored towards industrial, medical, and environmental applications. By manipulating and engineering these intricate pathways, scientists possess the capability to develop organisms endowed with perform specific functions, create new bio-based products, and even tackle global challenges such as pollution and resource scarcity.

Fig 1. Direct combinatorial pathway optimization.Fig 1. Direct combinatorial pathway optimization. (Coussement P, et al., 2017)

Service Offering

Our services include but not limited to the following:

Custom Pathway Design

Designing novel pathways tailored to clients' specific needs and desired end products.

Metabolic Engineering

Modifying existing pathways or creating new ones to enhance production yields and efficiency.

Host Organism Selection

Recommending suitable host organisms based on the pathway requirements and desired outputs.

Strain Construction

Assembling the genetic components and integrating them into the chosen host organism.

Pathway Optimization

Iteratively fine-tuning the pathway for optimal performance through experimental testing and computational modeling.

Fig 2. Key steps involved in the gene editing process.


Identifying the biological pathway that produces the desired compound or function.

Pathway Assembly

Integrating the selected genes into the host organism's genome or other expression systems.

Balancing and Fine-tuning

Adjusting enzyme expression levels and pathway intermediates to achieve optimal performance and prevent bottlenecks.

Gene Selection and Manipulation

Choosing the appropriate genes and enzymes to construct the pathway, and potentially modifying them for improved efficiency.

Metabolic Engineering

We will validate the performance of the assay by testing it with known positive and negative samples and measuring sensitivity, specificity, and reproducibility.

Fig 2. Key Steps in pathway design and optimization.

Our Advantages

Fig 3. Expertise.


A team of skilled scientists with expertise in synthetic biology, metabolic engineering, and pathway optimization.

Fig 4. Collaboration.


Ability to collaborate with clients to understand their needs and deliver tailored solutions.

Fig 5. Efficiency.


Streamlined processes that lead to quicker turnaround times for pathway development and optimization.

Fig 6. Computational Tools.

Computational Tools

Access to advanced computational tools for pathway design and prediction.

Biosynsis offers a wide range of cutting-edge technologies and has an experienced scientific team of biologists, bioinformaticians, and chemists dedicated to helping synthetic biology researchers around the world solve any challenges they encounter in their projects. If you have any specific needs, please feel free to contact us for more details.


  1. Coussement P.; et al. Direct combinatorial pathway optimization. ACS synthetic biology, 2017, 6(2): 224-232.
Please note that all services are for research use only. Not intended for any clinical use.

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