CD Biosynsis has built an advanced synthetic biology platform with the ability to help our customers achieve breakthroughs in the enhanced microbial production and the cell-free production of butanediol. We combine genetic engineering, metabolic engineering, and cell-free synthetic biochemistry to achieve high titer and high yield production of butanediol to meet the needs of diverse industries.
Advantages of Synthetic Biology-Driven Butanediol Production
Butanediol (BDO) with the molecular formula C4H10O2 refers to all the isomers of butylene glycol. BDO is an important organic compound that is widely used in different industries. Petroleum-derived BDO produced from substrates such as acetylene and propylene, is mainly produced via catalytic hydrogenation, which is a tedious and difficult process. Coupled with the instability of these substrates and the fact that the petroleum-based production is a costly process, there is a need to develop a commercial process for the biological production of alternatives to petroleum-derived BDO from renewable resources. The emerging enabling technologies and synthetic biology-inspired strategies allow the redesign, engineering, and optimization of novel biological pathways, enabling the modular development of engineered microorganisms for successful sustainable biological production of butanediol.
Figure 1. Comparison of chemical and biological processes for the production of 2,3-BD. (Song C W, et al., 2019)
What We Provide
Leveraging our powerful synthetic biology platform, CD Biosynsis can provide our customers with custom synthetic biology services to help them develop effective strategies to achieve efficient biosynthesis of butanediol.
Featured Services
- Engineering of pathway and channeling of metabolic flux towards BDO to achieve enhanced production in native BDO producing microorganisms.
- Construction and optimization of heterologous biosynthetic pathways for BDO production in non-native BDO producing microorganisms.
- Blocking competing pathways and overexpression of rate-limiting enzymes to minimize by-product formation and improve the yield of BDO.
- Development of metabolic engineering strategies to achieve improved BDO productivity from inexpensive substrates such as crude glycerol.
Key Strategies and Technologies
Deliverables
- Butanediol-producing microorganisms.
- High-quality butanediol products, including 1,2-butanediol, 1,3-butanediol, 1,4-butanediol, and 2,3-butanediol.
How We Can Help
CD Biosynsis offers various enabling technologies and tools in synthetic biology to help our customers solve any problems that may arise in the biological production of butanediol. Our scientists are committed to harnessing the power of synthetic biology to achieve high yield, economical and sustainable BDO production.
Development of Synthetic Biology Chassis for BDO Production
We have developed a synthetic biology toolbox including a wide range of techniques for different microbial species to help our customers identify key genes and biosynthetic pathways for improved microbial production of BDO. Our scientists can help our customers choose the right host strain for chassis development from the following table. If you are interested in other microbial strains, please fill out the online inquiry form and tell us more about your project.
| Bacillus amyloliquefaciens |
Bacillus licheniformis |
Bacillus subtilis |
Corynebacterium glutamicum |
Enterobacter aerogenes |
| Enterobacter cloace |
Escherichia coli |
Klebsiella oxytoca |
Klebsiella pneumoniae |
Lactococcus lactis |
| Paenibacillus polymyxa |
Saccharomyces cerevisiae |
Serratia marcescens |
Synechococcus elongatus |
Zymomonas mobilis |
Construction of Microbial Cell Factories for BDO Production
We have the ability to help our customers overcome genetic and metabolic engineering challenges of chassis strains. Our advanced computational tools allow for de novo pathway design in engineered microorganisms to enhance BDO production capacity. We can help design and develop microbial cell factories to meet the requirements for large-scale production of butanediol.
Development of Cell-Free Systems for BDO Production
Our expertise and experience enable us to develop cell-free systems as an alternative to microbial BDO production. Our multidisciplinary team can help our customers achieve high-yield BDO production by designing synthetic enzyme cascades in cell-free systems.
Applications of Butanediol
CD Biosynsis provides customized solutions for synthetic biology-driven butanediol production to meet a wide range of applications from chemical to energy industries.
- Used as a key intermediate for the production of various chemicals.
- Used as a building block and a precursor of many polymers and resins, such as degradable plastics, polyesters, polyurethanes, spandex fibers, and elastic fibers.
- Used in the manufacture of food additives, flavors, and fragrances.
- Used as a solvent in cosmetics.
- Used as a humectant in pharmaceutical products.
- Used as a liquid fuel.
- Used to manufacture printing inks, floor stripper, paint thinner, perfumes, synthetic rubber, fumigants, antifreeze agents and many other products.
Want to Learn More?
As a rapidly growing synthetic biology company, CD Biosynsis is committed to helping our customers meet the growing and evolving demand for bio-based chemical production. All of our deliverables will undergo a rigorous quality testing process to ensure the quality and reliability and can be delivered on time. If you are interested in our services or have any further questions, please do not hesitate to contact us.
References
- Song C W, et al. Microbial production of 2, 3-butanediol for industrial applications. Journal of Industrial Microbiology and Biotechnology, 2019, 46(11): 1583-1601.
- Liu H & Bowie J U. Cell-free synthetic biochemistry upgrading of ethanol to 1, 3 butanediol. Scientific reports, 2021, 11(1): 1-10.
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