CD Biosynsis is committed to helping customers achieve high succinic acid production at low pH by building more robust microbial hosts or developing low-cost feedstock-based fermentation processes.
Advantages of Synthetic Biology-Driven Succinic Acid Production
Succinic acid is an intermediate product of the tricarboxylic acid cycle as well as a fermentation product of anaerobic metabolism. Succinic acid is widely found in humans, animals, plants, and microorganisms, and is a highly promising C4 platform chemical that has been widely used in food, chemical, pharmaceutical, surfactant, biodegradable plastic, and other fields. Traditional production of succinic acid uses petroleum as raw material, which has the disadvantages of high prices and high pollution. Due to the depletion of fossil fuels and environmental concerns, the biosynthesis of succinic acid has received increasing attention. The main source of raw material for succinic acid biosynthesis is maize, which not only provides the carbohydrates necessary for the growth of the bacterium but also provides a cheap source of carbon. The production of succinic acid by synthetic biology method has the advantages of low raw material cost and environmental friendliness.
Figure 1. Succinic acid production biosynthetic pathways. (Liu X, et al., 2022)
What We Provide
Our scientists have strong background knowledge in synthetic biology and extensive experience in chemical biosynthesis. We are able to help our customers solve problems in succinic acid biosynthesis based on synthetic biology platforms and using bioengineering techniques.
Genetic Engineering
We are able to use genetic engineering tools to knock out genes encoding by-products in the fermentation pathway and develop high succinic acid producing strains to increase succinic acid production.
Metabolic Engineering
We are able to use metabolic engineering tools to improve the main metabolic pathways for succinate production such as the tricarboxylic acid cycle reduction branch, the oxidation branch and the glyoxalate pathway.
Genomic Analysis
We are able to predict the yield of succinic acid and analyze the reasons affecting its yield based on a multi-omics platform using tools such as genomic analysis and metabolomic analysis.
Deliverables
- Efficient cell factory for succinic acid production.
- Succinic Acid.
How We Can Help
Strain Improvement
We are able to apply genetic engineering tools to modify existing strains for succinic acid production. We expect to work with our customers to solve the problems of low succinic acid production capacity of natural strains, variety of fermentation products, and poor tolerance of strains to sugar or succinic acid. We are able to develop high succinic acid-producing strains through targeted mutations, genetic modifications, genome editing, and other technologies. For example, the overexpression of genes that promote succinic acid production, and the development of strains that can utilize amino acids to produce succinic acid. The following table shows the strains for which we can provide succinic acid biosynthesis development services. If the strain you are interested in is not on the table, please contact us directly.
| Saccharomyces cerevisiae |
Actinobacillus succinogenes |
Corynebacterium glutamicum |
| Escherichia coli |
Mannheimia succiniciproducens |
Yarrowia lipolytica |
Metabolic Evolution of Pathways for High-Yield Succinic Acid Production
Metabolic evolution refers to the metabolic rearrangement of imbalances compensated by the adaptation of the organism. We are able to help our customers activate the pentose phosphate pathway and transhydrogenase activity to increase succinate production in microbial strains.
Fermentation Process Improvement
We are able to help our customers optimize the batch fermentation process, the make-up batch fermentation process, and the continuous fermentation process involved in the production of succinic acid. Based on a precision fermentation platform, we improve the production efficiency of succinic acid strains and reduce the total cost of production.
Applications of Succinic Acid
CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive succinic acid production and meet the needs of customers in a variety of industries.
- Used as an ideal acidifier in food processing.
- Used as a preservative, pH adjuster, and co-solvent.
- Used in the pharmaceutical industry as a synthetic antidote, diuretic, sedative, hemostatic agent, etc.
- As a good surfactant.
- Used in the production of depilatories, toothpaste, and cleaning agents.
- Treatment of seeds in agricultural production to improve germination rates.
- As an additive to herbicides.
Want to Learn More?
CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in succinic acid production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.
References
- Liu X, et al. Biosynthetic Pathway and Metabolic Engineering of Succinic Acid. Front Bioeng Biotechnol. 2022 Mar 8; 10: 843887.
- Dessie W, et al. Opportunities, challenges, and future perspectives of succinic acid production by Actinobacillus succinogenes. Appl Microbiol Biotechnol. 2018 Dec; 102(23): 9893-9910.
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