Codon optimization is a crucial technique used to strategically modify the genetic code of a gene in order to enhance protein expression in a specific host organism. By fine-tuning codon usage, we can significantly improve protein production yield, enhance protein functionality, and increase overall efficiency.
The main purpose of Codon optimization is to increase the expression level of recombinant proteins. In common expression hosts, the frequency of use of different codons varies, and lower frequency of use of certain codons may limit protein expression. By adjusting the codon sequence of recombinant genes, the expression efficiency of proteins in specific hosts can be improved, thereby increasing protein production.
Codon frequencies in human (VP Mauro, et al.,2014)
Highlights
- Customized Codon Optimization: Our team of experienced experts will work closely with you to understand your unique requirements and goals. We will then develop a customized codon optimization strategy that is optimized for your specific needs.
- Enhanced Protein Expression: With our advanced algorithms and extensive knowledge in the field, we can maximize protein expression levels to achieve optimal results. By carefully selecting and modifying codons, we can enhance the translation efficiency and overall performance of your target protein.
- Multi-Host Compatibility: Our codon optimization services are available for a wide range of host organisms, including bacteria, yeast, plants, and mammalian cells. Whether you are working with a standard host organism or a non-standard one, our team has the expertise to optimize codon usage effectively.
- Quality Assurance: We understand the importance of quality in scientific research and production. Therefore, we ensure the highest quality standards throughout the codon optimization process. Our team conducts rigorous quality control checks at every stage to guarantee the accuracy and reliability of the optimized gene sequence.
Service Process
Case Studies
FAQs
Service Process
Our service process is designed to provide a seamless experience and deliver exceptional results. Here is a step-by-step overview of our service process:
- Project Consultation: We begin by conducting a thorough project consultation to gain a clear understanding of your requirements, goals, and target organism. This consultation allows us to tailor our codon optimization strategy specifically to your needs.
- Sequence Analysis: Our team of experts performs a detailed analysis of your gene sequence. We identify areas for optimization and determine the most effective codon modifications to enhance protein expression.
- Codon Optimization: Using state-of-the-art algorithms and our in-depth knowledge of codon usage patterns, we optimize the codon usage in your gene sequence. This optimization process involves strategically replacing codons with more favorable alternatives to improve protein expression.
- Gene Synthesis: Once the optimized gene sequence has been approved, we proceed with gene synthesis. Our team utilizes cutting-edge synthesis techniques to accurately synthesize the optimized gene sequence for further experimentation or production.
- Delivery and Support: We understand the importance of timely delivery. We provide prompt and reliable delivery of the optimized gene sequence, ensuring that you have the necessary materials for your research or production endeavors. Additionally, we offer comprehensive technical support to address any questions or concerns you may have along the way.
To learn more about our Codon Optimization services or to discuss your specific project, please feel free to contact us at info@biosynsis.com or call us at +1-631-637-0420. Our team of experts is ready to assist you and provide the guidance you need to achieve your research or production goals.
Case Studies
Thirty native and codon-optimized (synthetic) human SDR genes were expressed in E. coli BL21(DE3)-R3, with an induction temperature of 18 o C, and their expression and solubility profiles were compared. All of the experiments mentioned were carried out independently in triplicate. Expression was scored by the visualization of a band of the correct size by SDS–PAGE. Presence of a protein band in the total cell lysate which is not seen in control cultures (E. coli cells bearing expression constructs for differently-sized proteins) is scored as ‘‘expression”; the recovery of soluble protein after Ni-affinity purification is referred to as ‘‘solubility”. Improved expression and/or solubility was identified as an increase in the intensity of the band or better consistency seen between the three experiments and is therefore not fully quantitative.
Fig. 1 shows the result of an experiment with three genes, DHRS4, HADH2 and HSD17B4, in which codon optimization had a significant impact on expression and, at least in the case of HSD17B4, on the recovery of soluble protein. (The recovery of soluble protein seen for DHRS4 corresponds to yields of 1–2 mg/L of culture, while that of HSD17B4 corresponds to >10 mg/L.)
Fig. 1. Comparison of expression levels of native and synthetic genes in BL21(DE3)-R3. (NA Burgess-Brown, et al.,2008)
FAQs
Here are some frequently asked questions about Codon Optimization:
Q: How long does the codon optimization process take?
A: The duration of the process varies depending on the complexity of the gene and the specific requirements of the project. Our team will work closely with you to provide an estimated timeline based on your project's details.
Q: Can you optimize codons for non-standard host organisms?
A: Absolutely! We have extensive experience in optimizing codons for a wide range of host organisms. Whether you are working with a standard host organism or a non-standard one, our team has the expertise to optimize codon usage effectively.
Q: What are the potential benefits of codon optimization?
A: Codon optimization offers several potential benefits, including increased protein expression levels, improved protein functionality, enhanced translation efficiency, and overall better performance in the target organism. By fine-tuning codon usage, we can unlock the full potential of your gene of interest.
Q: Do you provide gene synthesis services as well?
A: Yes, we offer gene synthesis services to complement our codon optimization expertise. Our team can synthesize the optimized gene sequence for your downstream applications, providing you with a complete solution for your research or production needs.
We are committed to providing exceptional Codon Optimization services that meet the highest standards of quality and precision. Contact us today to learn more and embark on a successful journey of enhancing protein expression in your target organism.
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