CD Biosynsis is committed to integrating advanced protein engineering, metabolic engineering, and gene circuit design technologies to optimize membrane protein synthesis systems and further improve the personalization, diversification, and stability of membrane protein synthesis.
Membrane proteins are functionally rich, involved in many cellular reactions, and play a crucial role in the life activities of humans and other species. Membrane proteins can be classified into peripheral membrane proteins, integral membrane proteins, and lipid-anchored membrane proteins. Membrane proteins have become a focus of research in the fields of protein science, structural biology, medicine, and pharmacology due to their important applications in industry, environment, and defense. The biosynthesis of membrane proteins consists of two main components, which are protein expression and subsequent localization and assembly on biological membranes. Currently, the expression of membrane proteins is usually achieved using heterologous expression systems.
Figure 1. Membrane protein biogenesis in prokaryotes. (Freigassner M, et al., 2009)
Based on the synthetic biology platform, our scientists are dedicated to exploring the application of genetic circuits, protein engineering, and other technologies in the process of membrane protein production.
Membrane Protein Synthesis in Cell-Free Systems
The cell-free protein synthesis system is a classical protein synthesis technique. We have successfully developed a variety of cell-free protein expression systems for extracts, each with its own advantages and disadvantages, and customers can choose the right system according to the target membrane protein to be expressed. The cell-free protein synthesis systems we have successfully constructed are listed below.
|Escherichia coli cell extract||Saccharomyces cerevisiae cell extract||Wheat germ cell extract||Tobacco cell extract|
|Rabbit reticulocyte lysate||Chinese hamster cell extract||Insect cell lysate||Recombinant elements system|
We have successfully constructed a variety of microbial chassis for membrane protein production. At the same time, we use different strategies to optimize the membrane protein production process based on the influencing factors in the process. Currently, most strategies to optimize membrane protein expression focus on modifying the membrane protein itself and optimizing microbial culture conditions. The following are the microbial chassis and optimization strategies we can provide for membrane protein production.
|Escherichia coli||Lactococcus lactis||Bacillus subtilis|
CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive membrane protein production and meet the needs of customers in a variety of industries.
CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in membrane protein production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.