Introduction of Plant Metabolic Pathway Engineering
Plant metabolic pathway engineering is a powerful tool that has revolutionized the field of synthetic biology. It involves the modification of plant metabolic pathways using genetic engineering techniques to produce high-value compounds, such as pharmaceuticals, industrial chemicals, and biofuels. The use of synthetic biology tools in plant metabolic engineering has enabled the production of these valuable compounds in a cost-effective and sustainable manner.
CD Biosynsis has been working in the field of plant metabolic pathway engineering for years. Our expertise in synthetic biology has enabled us to develop innovative solutions for the production of high-value compounds using plant metabolic engineering techniques.
Figure 1: Heterologous reconstitution of plant pathways in yeast, as exemplified by reconstitution of opiate biosynthetic pathways in yeast. (Tatsis E. C., O’connor S. E. 2016)
Our Services for Plant Metabolic Pathway Engineering
At CD Biosynsis, we offer a range of services for plant metabolic pathway engineering. Our services include:
Metabolic Pathway Analysis
Our metabolic pathway analysis service involves using computational models like constraint-based models and genome-scale models to analyze plant metabolic pathways. We identify key regulatory points and bottlenecks, allowing us to optimize metabolic pathways to increase the yield of desired products. Our expertise in metabolic modeling enables us to provide accurate and reliable predictions of the behavior of complex metabolic systems.
Gene Synthesis and Cloning
Our gene synthesis and cloning service involves the construction of metabolic pathways. We design and construct complex metabolic pathways using synthetic biology tools such as Gibson Assembly and Golden Gate Assembly. We use cutting-edge DNA synthesis technologies to synthesize genes, which are then cloned into plant expression vectors for the production of desired compounds. Our expertise in genetic engineering allows us to efficiently produce high-value compounds using plant metabolic engineering techniques.
Plant Transformation
We offer a plant transformation service that includes the transformation of a wide range of plant species, including Arabidopsis thaliana, Nicotiana benthamiana, and tomato. We use Agrobacterium-mediated transformation and biolistic transformation techniques to introduce genetic constructs into plants for the production of desired compounds. Our extensive experience in plant transformation allows us to efficiently produce high-value compounds using plant metabolic engineering techniques.
Metabolite Analysis
Our metabolite analysis service involves the quantification of the levels of desired compounds in plant tissues. We use high-performance liquid chromatography (HPLC) and mass spectrometry (MS) techniques to identify and quantify metabolites in plant samples. This allows us to optimize metabolic pathways for the production of desired compounds.
Our Advantages
- Expertise in Synthetic Biology
- Extensive experience in synthetic biology
- Ability to design and construct complex metabolic pathways
- Expertise in gene synthesis, cloning, and plant transformation
- Cost-Effective Solutions
- Optimization of metabolic pathways to increase the yield of desired compounds
- Reduction of production costs
- Efficient production of high-value compounds using plant metabolic engineering techniques
- Sustainable Solutions
- Use of plant-based systems for the production of high-value compounds
- Renewable and biodegradable systems
- Environmentally friendly alternative to traditional chemical synthesis methods.
CD Biosynsis offers a range of services, including metabolic pathway analysis, gene synthesis and cloning, plant transformation, and metabolite analysis. Our services enable the efficient and cost-effective production of high-value compounds using plant metabolic engineering techniques. Contact us and find more details about our services for plant metabolic pathway engineering.
References
- Tatsis E. C., O’connor S. E. New developments in engineering plant metabolic pathways. Current Opinion in Biotechnology, 2016, 42: 126-132.
- Capell T., Christou P. Progress in plant metabolic engineering. Current Opinion in Biotechnology, 2004, 15(2): 148-154.
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