Custom miRNA Synthesis Service

MicroRNAs (miRNAs) are small, non-coding RNA molecules (18-25 nt) that regulate gene expression by targeting mRNAs for degradation or translational repression. Customized synthetic miRNA molecules, including miRNA Mimics (agonists, agomirs) and miRNA Inhibitors (antagonists, antagomirs), are powerful tools for studying gene function and developing novel therapeutics. Due to their regulatory role, miRNA function is highly dependent on sequence fidelity and chemical stability within cellular and in vivo environments.

CD Biosynsis provides specialized Custom miRNA Synthesis Service focused on achieving the highest purity and incorporating necessary chemical modifications for maximum efficacy. We synthesize both single-stranded Inhibitors and double-stranded Mimics, utilizing proprietary modification patterns like 2'-O-Methyl (2'-OMe) and Phosphorothioate (PTO) backbone alterations to ensure resistance against RNase degradation. Our service offers a wide range of purification options, including HPLC for In Vitro use and HPLC plus endotoxin testing for In Vivo grade material , guaranteeing reliable results for your functional genomics and drug development studies.

Highlights

Specialized capabilities for superior miRNA molecules:

• Enhanced Stability: Routine incorporation of 2'-O-Methyl (2'-OMe) modifications on ribose sugars to confer strong resistance to RNase degradation in serum and tissue.
• Therapeutic Grade Options: Capability to produce In Vivo Grade miRNA with HPLC purification, endotoxin testing, and cholesterol conjugation for targeted delivery.
• Dual Product Range: Synthesis of both miRNA Mimics (double-stranded agonists) and miRNA Inhibitors (single-stranded antagonists, antagomirs).
• Rigorous QC: All products are verified by Mass Spectrometry (MS) to confirm the exact molecular weight and HPLC for guaranteed purity ( >90% standard).

Applications

Custom miRNA tools drive functional studies and drug development:

miRNA Loss-of-Function Studies

           

Using miRNA Inhibitors (Antagomirs) to block endogenous miRNA function, enabling the study of target gene upregulation and disease mechanisms.

miRNA Gain-of-Function Studies

Introducing miRNA Mimics (Agomirs) into cells to enhance the function of a specific miRNA, testing its role in cell growth, differentiation, or apoptosis.

Drug Target Validation

Modulating specific miRNAs to validate their regulatory networks and determine their potential as therapeutic targets or biomarkers for disease intervention.

In Vivo Pharmacology

Utilizing In Vivo grade Agomirs with targeting modifications (e.g., cholesterol conjugation) for animal model studies and preclinical testing.

Products & Modifications

Essential products and key chemical modifications for functional miRNA research:

miRNA Mimics (Agomirs)

Chemically synthesized double-stranded RNA designed to replicate endogenous mature miRNA function, delivered as ready-to-transfect duplexes.

miRNA Inhibitors (Antagomirs)

Single-stranded, highly modified RNA that tightly binds and sequesters endogenous miRNA to block its function, optimized for stability.

2'-O-Methyl (2'-OMe) Modification

The standard modification for miRNA stabilization, offering enhanced RNase resistance without significantly altering RNA structure or targeting.

Phosphorothioate (PTO) Backbone

Replacement of a non-bridging oxygen atom with sulfur, further enhancing nuclease resistance and improving pharmacokinetic properties for in vivo use.

Targeting Ligands (Cholesterol, GalNAc)

Chemical conjugation of cholesterol or other delivery ligands, enabling improved cellular uptake and targeted delivery in animal models.