Introduction
Recombinant protein expression in CHO cells is an important aspect of synthetic biology that has revolutionized the pharmaceutical industry. Therapeutic proteins require proper folding and post-translational modifications to be effective and biologically active. CHO cells are the most frequently used host for commercial production of therapeutic proteins due to their ability to perform these modifications. However, an unpredictable decrease in protein productivity during the time required for scale-up impairs process yields, time, finance, and regulatory approval for the desired product. Therefore, it is important to assess cell lines at stages throughout the period of long-term culture in terms of productivity and various molecular parameters including plasmid and mRNA copy numbers and location of the plasmid on the host cell chromosome.
At CD Biosynsis, we offer services for improving the stability of CHO cells and enhancing recombinant protein expression. Our services are designed to address the challenges faced by the pharmaceutical industry during the production of therapeutic proteins.
Figure 1: CHO Cell Community Effort Toward Better Biopharmaceutical Production Costs. (Harcum S. W., Lee K. H. 2016)
Our Services for Genetic Engineering of CHO Cells Modification
CHO Cell Line Development
Our CHO cell line development service is customized to meet the specific needs of our clients. We use advanced genetic engineering techniques to optimize gene expression and enhance protein folding, resulting in improved protein yields. Our team of experts can develop stable CHO cell lines that can produce high levels of recombinant proteins. The process of CHO cell line development involves the following steps:
- Design plasmid vector carrying gene of interest
- Select appropriate CHO cell line
- Perform transfection
- Select stable cell lines that can produce high levels of the recombinant protein
Our expertise in cell line development allows us to optimize gene expression and enhance protein folding, resulting in improved protein yields.
Protein Expression Optimization
We use various methods to optimize the expression of your recombinant protein by modifying factors such as media composition, culture conditions, and transfection methods. Our team of experts can optimize protein expression for both stable and transiently transfected CHO cells. The process of protein expression optimization involves the following steps:
- Analyze the expression level of the protein of interest
- Identify any factors that may be limiting its expression
- Modify factors such as media composition, culture conditions, and transfection methods to enhance protein expression
- Optimize protein expression for both stable and transiently transfected CHO cells
Cell Line Characterization
We use various methods such as western blotting, ELISA, real-time PCR, and fluorescent in situ hybridization (FISH) to analyze the stability of your recombinant CHO cells over a long period of culture. The process of cell line characterization involves the following steps:
- Evaluate the stability of the CHO cell line over long-term culture
- Use various methods such as western blotting, ELISA, real-time PCR, and FISH to analyze the stability of the recombinant CHO cells
- Identify potential issues early on and ensure the stability of the CHO cell line over time
Process Development and Scale-up
We can design a scalable process that meets your specific needs and provide guidance on process optimization and scale-up. The process of process development and scale-up involves the following steps:
- Analyze the current production process and identify areas for improvement
- Design a scalable process that meets the specific needs of the client
- Provide guidance on process optimization and scale-up
- Help increase process yields, reduce production costs, and improve the efficiency of the production process.
Our Advantages
- Expertise in CHO Cell Line Development
- Optimal Protein Expression
- Stable CHO Cell Lines
CD Biosynsis uses advanced genetic engineering techniques and bioprocessing technologies to optimize gene expression and enhance protein folding, resulting in improved protein yields and quality. Our services are customized to meet the specific needs of our clients and are designed to address the challenges faced by the pharmaceutical industry during the production of therapeutic proteins. Contact us today to learn more about our services and how we can help you achieve your production goals.
References
- Ritacco F.V, et al. Cell culture media for recombinant protein expression in Chinese hamster ovary (CHO) cells: History, key components, and optimization strategies. Biotechnology progress, 34(6), 1407–1426.
- Wang W., et al. RETRACTED ARTICLE: Impact of different promoters, promoter mutation, and an enhancer on recombinant protein expression in CHO cells. Scientific Reports, 2017, 7(1): 10416.
- Harcum S. W., Lee K. H. CHO Cells Can Make More Protein. Cell Systems, 2016, 3(5): 412-413.
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