Recombinant protein expression in CHO cells is an important aspect of synthetic biology that has revolutionized the pharmaceutical industry. Therapeutic proteins require proper folding and post-translational modifications to be effective and biologically active. CHO cells are the most frequently used host for commercial production of therapeutic proteins due to their ability to perform these modifications. However, an unpredictable decrease in protein productivity during the time required for scale-up impairs process yields, time, finance, and regulatory approval for the desired product. Therefore, it is important to assess cell lines at stages throughout the period of long-term culture in terms of productivity and various molecular parameters including plasmid and mRNA copy numbers and location of the plasmid on the host cell chromosome.
At CD Biosynsis, we offer services for improving the stability of CHO cells and enhancing recombinant protein expression. Our services are designed to address the challenges faced by the pharmaceutical industry during the production of therapeutic proteins.
Figure 1: CHO Cell Community Effort Toward Better Biopharmaceutical Production Costs. (Harcum S. W., Lee K. H. 2016)
Our CHO cell line development service is customized to meet the specific needs of our clients. We use advanced genetic engineering techniques to optimize gene expression and enhance protein folding, resulting in improved protein yields. Our team of experts can develop stable CHO cell lines that can produce high levels of recombinant proteins. The process of CHO cell line development involves the following steps:
Our expertise in cell line development allows us to optimize gene expression and enhance protein folding, resulting in improved protein yields.
We use various methods to optimize the expression of your recombinant protein by modifying factors such as media composition, culture conditions, and transfection methods. Our team of experts can optimize protein expression for both stable and transiently transfected CHO cells. The process of protein expression optimization involves the following steps:
We use various methods such as western blotting, ELISA, real-time PCR, and fluorescent in situ hybridization (FISH) to analyze the stability of your recombinant CHO cells over a long period of culture. The process of cell line characterization involves the following steps:
We can design a scalable process that meets your specific needs and provide guidance on process optimization and scale-up. The process of process development and scale-up involves the following steps:
CD Biosynsis uses advanced genetic engineering techniques and bioprocessing technologies to optimize gene expression and enhance protein folding, resulting in improved protein yields and quality. Our services are customized to meet the specific needs of our clients and are designed to address the challenges faced by the pharmaceutical industry during the production of therapeutic proteins. Contact us today to learn more about our services and how we can help you achieve your production goals.
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.