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Trusted by Leading Research & Pharma Institutions

Glycoprotein Production Services

Produce high-quality recombinant glycoproteins with authentic human-like glycosylation patterns using state-of-the-art mammalian cell expression systems. From therapeutic antibodies to complex multi-domain proteins, we deliver optimal folding, sialylation, and efficacy.

Human-like Glycosylation
High Success Rate
Full QC Package
Learn More

Trusted by leading research and pharmaceutical institutions

Harvard
Pfizer
MIT
Roche
Stanford
Novartis

Why Choose Us

Authentic human-like glycosylation
Glycoengineering options available
Comprehensive analytical support
Expert consultation included

CHO Cells

Gold standard for therapeutic proteins

HEK293 Cells

Rapid expression for screening

Glycoengineering

Customized glycosylation profiles

Success Rate
90%+
Overview Technology Specifications Workflow Applications FAQ
Service Overview

Why Choose Our Glycoprotein Production Service?

Glycosylation is the most complex and heterogeneous form of protein post-translational modification. Our mammalian cell platforms deliver authentic human-like glycosylation patterns essential for therapeutic efficacy.

Mammalian Cell Platforms

Chinese Hamster Ovary (CHO) and HEK293 cells provide authentic human-like N-glycosylation, ensuring proper protein folding and biologically active glycan structures essential for therapeutic applications.

  • CHO cells for therapeutic production
  • HEK293 for rapid screening
  • Proper disulfide bond formation

Glycoengineering Options

Customize glycosylation profiles to optimize therapeutic efficacy. Options include afucosylation, enhanced sialylation, bisecting GlcNAc, and controlled glycan heterogeneity for specific applications.

  • Afucosylation for enhanced ADCC
  • Controlled sialylation levels
  • Bisecting GlcNAc incorporation

Therapeutic Antibodies

Full-length IgG antibodies with optimal glycosylation for effector functions.

Fc Fusion Proteins

Engineered fusion proteins with controlled glycosylation for extended half-life.

Complex Glycoproteins

Multi-domain proteins requiring authentic glycosylation for structural integrity.

Ready to Produce Your Glycoprotein?

Get expert consultation and customized quote for your project.

Technology Platform

Advanced Expression Technology

Cutting-edge mammalian cell platforms optimized for high-yield glycoprotein production.

CHO Cell Expression System

Chinese Hamster Ovary cells are the gold standard for therapeutic glycoprotein production. Our optimized CHO platforms deliver high yields with consistent, human-like glycosylation.

High Yield FDA-Approved

HEK293 Expression System

HEK293 cells offer rapid expression for screening and early development. Ideal for quick turnaround and glycan profile optimization before scale-up to CHO.

Rapid Screening

Glycoengineering Platform

Engineered glycoforms for optimized therapeutic properties. Control sialylation, fucosylation, and glycan branching to enhance efficacy and half-life.

Custom Glycans ADCC Enhanced

Quality Control

SEC-HPLC Purity and aggregation
Endotoxin LAL testing
Glycan Glycan profiling
MS Mass spectrometry

Glycosylation Analysis

Sialylation Sialic acid content
Fucosylation Core Fucose analysis
G0/G1/G2 Glycan distribution
Charge Charge variant analysis
Specifications

Flexible Options for Diverse Needs

Comprehensive specifications to meet your research requirements.

Parameter CHO Cells HEK293 Cells
Typical Yield 1-10 g/L 0.1-1 g/L
Glycosylation Human-like, complex-type Human-like, high mannose possible
Scale Range 100 mg - 100+ g 1-100 mg
Turnaround Time 3-8 weeks 2-4 weeks
Glycoengineering Available Limited
Best For Therapeutic development Research, screening
Workflow

Streamlined Process from Design to Delivery

Our proven workflow ensures quality and efficiency at every stage.

1

Consultation

Project assessment and strategy

2

Expression

Cell culture and transfection

3

Purification

Multi-step purification

4

QC

Glycan and purity analysis

5

Delivery

Secure delivery with COA

Applications

Diverse Applications Across Research

Our glycoprotein production services support research in multiple fields.

Therapeutic Development

High-quality glycoproteins for therapeutic antibody development, biosimilar characterization, and novel biologics. Our CHO platforms meet regulatory requirements for clinical development.

  • Therapeutic antibodies
  • Fc fusion proteins
  • Biosimilar reference standards
  • ADC payloads
GMP
Compliant production

Research Applications

Recombinant glycoproteins for basic research, structural biology, and mechanism studies. HEK293 systems provide rapid turnaround for research applications.

  • Structural biology
  • Binding studies
  • Glycobiology research
  • Enzyme characterization
2-4
Weeks turnaround

Diagnostic Development

Glycoproteins for diagnostic assay development including ELISA standards, reference materials, and calibration controls. Consistent glycosylation ensures reliable assay performance.

  • ELISA standards
  • Calibration controls
  • Reference materials
  • Quality control reagents
90%+
Batch consistency
Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The glycan profile of their CHO-expressed antibodies was exactly what we needed. The sialylation levels and afucosylation were well-controlled. Excellent service."

S
Senior Scientist
Pharmaceutical Company

"Their glycoengineering platform allowed us to produce antibodies with enhanced ADCC. The detailed glycan analysis was comprehensive. Highly recommend for therapeutic development."

R
Research Director
Biotechnology Company

"Fast turnaround and excellent communication. The CHO cell platform delivered high yields with consistent quality. Our go-to partner for glycoprotein production."

L
Lead Researcher
Academic Research Institution
Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

89 Citations

Cell-free systems for accelerating glycoprotein expression and biomanufacturing

Hershewe J, Kightlinger W, Jewett MC. J Ind Microbiol Biotechnol. 2020.

Review of cell-free glycoprotein synthesis systems using natural and synthetic N-linked glycosylation pathways.

View DOI
26 Citations

Glycoengineering Chinese hamster ovary cells: a short history

Donini R, Haslam SM, Kontoravdi C. Biochem Soc Trans. 2021.

Comprehensive review of glycoengineering strategies in CHO cells for remodelling N-glycosylation.

View DOI
New

Immobilized enzyme cascade for targeted glycosylation

Makrydaki E, Donini R, Krueger A, et al. Nature Chemical Biology. 2024.

Continuous glycosylation platform using immobilized enzymes for controlled N-linked glycan production.

View DOI
New

Advances in bacterial glycoprotein engineering: A critical review

Li Z, Wang Y, Zhao X, et al. Biotechnology Advances. 2025.

Review of bacterial protein glycosylation technologies from synthetic biology and metabolic engineering perspectives.

View DOI
New

Production of eukaryotic glycoproteins using Expi293F cells

Greven JA, Brett TJ. Current Protocols. 2022.

Detailed protocols for glycoprotein production in Expi293F mammalian cells with proper post-translational modifications.

View DOI
FAQ

Frequently Asked Questions

Find answers to common questions about our service.

CHO cells produce higher yields (1-10 g/L) with complex human-like glycosylation, making them the gold standard for therapeutic protein production. HEK293 cells offer faster turnaround (2-4 weeks vs 3-8 weeks) with more human-like glycosylation patterns, ideal for research and early screening. Our team will recommend the optimal platform based on your application.
We offer multiple glycoengineering options: afucosylation for enhanced ADCC activity, controlled sialylation for optimized half-life, bisecting GlcNAc incorporation, and homogeneous glycan production. Our glycoengineered CHO platforms can produce specific glycoforms with consistent quality.
Standard glycosylation analysis includes glycan profiling, sialic acid content determination, fucosylation analysis, and G0/G1/G2 distribution. Mass spectrometry and charge variant analysis are included for comprehensive characterization. Detailed glycan maps are provided in the Certificate of Analysis.
HEK293 expression typically takes 2-4 weeks for small-scale production. CHO cell expression requires 3-8 weeks depending on scale and complexity. Expedited services are available for urgent projects. Contact us for a detailed timeline based on your specific requirements.
Yes, we have experience with challenging targets including multi-domain proteins, heavily glycosylated proteins, and proteins with complex disulfide bond patterns. Our platforms include strategies for optimizing expression, folding, and secretion to maximize success with difficult proteins.
We offer flexible scale options from milligram to multigram quantities. Small-scale expression (1-100 mg) is ideal for initial characterization and screening. Medium-scale production (100 mg - 1 g) supports most research applications. Large-scale production (1-100+ g) is available for therapeutic development.

Ready to Start Your Project?

Get a customized quote for your Glycoprotein Production Services project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

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