Custom Modified Oligos Synthesis Service

Custom Modified Oligonucleotides are synthetic nucleic acids that incorporate non-natural chemical groups, fluorescent dyes, quenchers, linkers, or backbone stabilizing modifications. These specialized molecules are vital tools in cutting-edge research, serving as advanced molecular probes, therapeutic agents (e.g., antisense), aptamers, and components of sophisticated nanotechnology and biosensors. The successful synthesis of these complex molecules requires specialized chemistry and rigorous purification protocols.

CD Biosynsis offers a comprehensive Custom Modified Oligos Synthesis Service , providing access to one of the industry's broadest catalogs of modifications, including over 100 fluorescent labels, quenchers, non-standard bases, conjugation linkers (e.g., thiols, amines), and stabilizing chemistries (e.g., LNA, PTO). Leveraging advanced solid-phase synthesis and mandatory HPLC purification, we guarantee the highest coupling efficiency and purity for your complex modified oligos, accelerating your diagnostic, therapeutic, and bioconjugation projects.

Highlights

Our expertise in complex chemical synthesis ensures reliable delivery of challenging modified oligos:

• Broad Modification Catalog: Access to over 500 catalog and non-catalog modifications, including functional groups (NH _2, SH), complex fluorophores (ATTO, Alexa Fluor), and exotic bases.
• Dual/Multiple Labeling: Expertise in synthesizing oligos with multiple labels (e.g., two different dyes, or a dye/quencher pair) at precise internal or terminal positions, verified by dual Mass Spectrometry.
• Stabilizing Chemistries: Routine synthesis of oligos incorporating LNA (Locked Nucleic Acid), 2'-O-Methyl, and Phosphorothioate (PTO) linkages for enhanced nuclease resistance and binding affinity.
• Guaranteed High Purity: Mandatory HPLC or PAGE purification for all modified oligos to ensure the functional integrity and high activity of the final product.

Applications

Modified oligos are driving innovation in diagnostics, therapeutics, and materials science:

Therapeutic Oligonucleotides

           

Synthesis of siRNA, ASO (Antisense Oligos), and miRNA inhibitors with backbone modifications (PTO, 2'-O-Me) for stability and delivery.

Aptamers & Bioconjugation

Production of high-affinity aptamers and oligos with reactive functional groups (Thiol, Azide) for conjugation to proteins, nanoparticles, or surfaces.

Biosensors & Nanotechnology

Synthesis of fluorescently labeled oligos and specialized structural oligos (e.g., sticky-end, hairpins) for DNA origami and molecular switches.

FRET & Single-Molecule Imaging

Precision synthesis of oligos with specific FRET pairs for distance measurement and high-brightness dyes for single-molecule microscopy (SMF).

Products & Solutions

Our expertise covers the most complex and advanced oligonucleotide modifications available.

Fluorescent & Quencher Labeling

Terminal or internal labeling with FAM, Cy3/Cy5, Alexa Fluor dyes, and BHQ quenchers, with confirmed high coupling efficiency.

Backbone & Sugar Modifications (ASO Grade)

Incorporation of PTO (Phosphorothioate), LNA, 2'-O-Methyl RNA, and Morpholino groups for therapeutic applications.

Reactive Functional Groups

Attachment of Amino (NH _2), Thiol (SH), Biotin, Phosphate, and Click Chemistry (Azide/Alkyne) linkers for covalent conjugation.

Non-Standard & Labeling Bases

Synthesis incorporating Inosine, Locked Nucleic Acid (LNA), Deaza bases, and various T and A internal labels.

Ultra-High Purity (PAGE & Dual HPLC)

Provision of ultra-pure oligos required for structure determination, X-ray crystallography, and NMR applications.