CD Biosynsis is committed to helping customers achieve continuous production of citric acid based on a synthetic biology platform, and to improve the efficiency and reduce the cost of production of citramalic acid.
Citramalic acid is an important organic acid that is widely distributed in plant, animal, and microbial cells. Natural citramalic acid is found in the fruits of plants such as lemons and pineapples and the bones, muscles, and blood of animals. Citramalic acid has a wide range of uses in food, chemical, pharmaceutical, construction, leather, detergent, and other industries. Currently, microbial fermentation is the main way to produce citramalic acid. The types of microorganisms used for industrial citric acid production are mainly Aspergillus and yeast. However, Aspergillus and yeast strains are mostly intolerant to high temperatures and require large amounts of cooling water to control the fermentation temperature during citramalic acid production. Therefore, synthetic biology-driven development services for citramalic acid production are of great importance.
Figure 1. Metabolic pathways for citramalate synthesis in Escherichia coli. (Parimi NS, et al., 2017)
Our scientists hope to help our customers further understand the citramalic acid production pathway of microorganisms through transcriptomic and proteomic analysis. We are able to use genetic engineering, protein engineering, and metabolic engineering tools to help our customers break through bottlenecks in citramalic acid biosynthesis.
We are able to use genetic engineering tools to help our customers select and breed citramalic acid producing strains with excellent traits.
We are able to optimize the metabolic pathways of natural citramalic acid producing strains using metabolic engineering tools to improve citramalic acid production.
We are able to optimize the citramalic acid synthesis pathway by using protein engineering tools to design key enzymes in the citramalic acid synthesis process that contain specific mutations.
We are able to help our customers construct drug-resistant, high-temperature, high-yield citramalic acid production strains using cell engineering tools.
We use proteomic analysis to help our customers gain insight into the mechanism of citramalic acid production at the protein expression level and to provide a basis for strain modification.
Development of Synthetic Biology Chassis for Citramalic Acid Production
Our citramalic acid production services are based on microbial fermentation methods. The following table shows the microbial chassis we can offer for citramalic acid production. If you have other microbial chassis of interest for citramalic acid production, please contact us directly.
|Aspergillus nige||Yarrowia lipolytic||Candida lipolytic||Escherichia coli|
Screening of Strains with Excellent Traits
Construction of Immobilized Fermentation System
We can help our customers to build and optimize immobilized fermentation systems. By establishing a fermentation process with a high concentration of citramalic acid-producing bacteria and uninterrupted production, we can save production and time costs for citric acid fermentation.
Morphological Regulation of Filamentous Fungi
The morphology of mycelium significantly affected the yield of citramalic acid-producing bacteria. We are able to use molecular biology techniques to regulate the expression of morphological genes to help our customers improve citramalic acid production.
CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive citramalic acid production and meet the needs of customers in a variety of industries.
CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in citramalic acid production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.