Introduction
Adaptive evolution is a powerful tool used in synthetic biology to improve the performance of microbial strains for various biotechnological applications. The technique involves the selection and propagation of genetic variants with desirable traits over multiple generations, resulting in the evolution of the microbial population towards a specific objective. One of the significant advantages of adaptive evolution is that it can be used to modify a strain's genome without the need for genetic engineering. This approach allows for the optimization of a strain's performance by selecting for natural genetic variation that confers a selective advantage under specific growth conditions.
CD Biosynsis has a mature technology and platform, which is the preferred choice for adaptive evolution services in the biotechnology industry.
Figure 1: Applications of adaptive laboratory evolution (ALE) in strain development for biomanufacturing and in basic science research. (Long C.P., Antoniewicz M.R. 2018)
Our Services for Adaptive Evolution
At CD Biosynsis, we offer a range of services for adaptive evolution, specifically designed to meet the needs of clients in the biotechnology industry. Our services for adaptive evolution can be divided into three main categories:
Strain Isolation Services
Our strain isolation services involve the identification of microbial strains with desirable traits, such as increased growth rates, improved product yields, and enhanced stress tolerance. We use a range of techniques to isolate these strains, including:
Screening of natural microbial populations
Selection of mutants
Genetic engineering
Strain Characterization Services
Once the desired strain has been isolated, we proceed with strain characterization. This involves the analysis of the strain's genetic and phenotypic properties, including:
Genome sequencing
Transcriptomics
Proteomics
Metabolic profiling
By characterizing the strain in detail, we can better understand its strengths and weaknesses and identify areas for improvement.
Strain Optimization Services
The next step in our adaptive evolution services is strain optimization. This involves the selection and propagation of genetic variants with desirable traits over multiple generations, resulting in the evolution of the microbial population towards a specific objective. We use a range of techniques to optimize strains, including:
Serial transfer
Chemostat culturing
Directed evolution
At CD Biosynsis, we have extensive experience in using adaptive evolution to optimize microbial strains for various industrial processes, such as biofuel production, bioremediation, and biopharmaceutical production. We work closely with our clients to understand their specific needs and develop customized solutions that meet their requirements. Our services are designed to provide maximum value to our clients, allowing them to optimize their microbial strains while minimizing costs.
Our Advantages
- Expertise
Our team of experts has extensive experience in using adaptive evolution to optimize microbial strains for various biotechnological applications.
- State-of-the-Art Equipment
We use state-of-the-art equipment and techniques to monitor the progress of adaptive evolution experiments. This includes:
High-throughput sequencing
Transcriptomics
Proteomics
These technologies allow us to track changes in the microbial population's genetic and phenotypic properties over time accurately.
- Customization
- Rapid Turnaround Time:
Minimum turnaround time is 6 weeks.
- Cost-Effective Solutions
Adaptive evolution is a powerful tool for improving the performance of microbial strains for various biotechnological applications. CD Biosynsis is a leading provider of adaptive evolution services, offering customized solutions to meet the specific needs of each client. Our team of experts, state-of-the-art equipment, and streamlined processes ensure that we deliver high-quality results quickly and efficiently. Contact us today to learn more about our adaptive evolution services and how we can help optimize your microbial strains for maximum performance.
References
- Long C.P., Antoniewicz M.R. How adaptive evolution reshapes metabolism to improve fitness: recent advances and future outlook. Current Opinion in Chemical Engineering, 2018, 22: 209-215.
- Xiang W., Shanwei L., et al. Adaptive evolution of microalgal strains empowered by fulvic acid for enhanced polyunsaturated fatty acid production. Bioresource Technology, 2019, 277: 204-210.
- Patyshakuliyeva, A., Arentshorst, M., et al. Improving cellulase production by Aspergillus niger using adaptive evolution. Biotechnology Letters, 2016, 38: 969–974.
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