CD Biosynsis is committed to using our expertise and experience to help our customers overcome challenges and obstacles in engineering mammalian cells as synthetic biology chassis. We work closely with our customers to expand the mammalian synthetic biology toolbox and advance the biological and medical applications of mammalian synthetic biology. We can also help our customers advance their cellular and molecular research in mammalian cells and address key questions in mammalian biology using synthetic biology technologies.
Background
In synthetic biology, an ideal chassis should be one organism that is well understood and does not have any unintended consequences after gene editing. However, a major problem for mammalian cell engineering is that unpredictable long-range interactions may always occur along the DNA in mammalian chromosomes. In addition, the enabling technologies underlying synthetic biology may not be feasible in mammalian cells. While pioneering synthetic biology research has focused on prokaryotic cells, more recent studies in mammalian cell lines are emerging. Some success has been achieved in genetic circuit engineering for mammalian cell-based applications. The focus of mammalian synthetic biology will begin to shift towards the exploration of new synthetic biology tools to exploit the potential of mammalian cells as synthetic biology chassis.
What We Can Do
CD Biosynsis offers a range of enabling techniques and is constantly developing novel synthetic biology tools to open up new opportunities for the engineering and application of mammalian cells. Our scientists working on mammalian synthetic biology have the ability to help our customers solve any problems encountered in their projects and bring their breakthrough ideas to life. Our capabilities include, but are not limited to:
| Tools for transcriptional control |
Zinc-finger-containing factors |
Transcription activator-like effectors |
CRISPR-based regulators |
Transcriptional gene switches |
| Tools for RNA control |
Aptamers |
Self-cleaving ribozyme |
siRNA- and miRNA-based regulators |
Post-transcriptional regulatory switches |
| Tools for protein regulation |
Ligand-induced degradation (LID) domain |
Modified degradation domain (DD) |
Ligand-induced stabilization |
Post-translational synthetic circuits |
| Tools for genome engineering |
Site-specific genome editing |
CRISPR-Cas systems |
Multiplexed genome engineering (MAGE) |
Synthetic gene expression circuits |
| Tools for signaling pathway engineering |
Ligand specificity engineering |
Modified intracellular receptor domains |
Modified membrane receptors |
Engineered split inteins |
Applications of Mammalian Chassis
CD Biosynsis' mammalian synthetic biology capabilities enable us to help our customers engineer mammalian chassis for a wide range of basic research and medical applications. We are well positioned to support the emerging applications of mammalian synthetic biology.
Mammalian Chassis for Basic Research Applications
We use our mammalian synthetic biology toolbox to help our customers explore complex cellular and molecular regulatory mechanisms in mammalian cells.
- The study of molecular mechanisms, such as genomic and epigenomic mechanisms.
- The study of gene regulatory networks.
- The study of cell signaling.
- The study of multicellular behavior, such as cellular development and differentiation and cell-to-cell communication.
Mammalian Chassis for Medical Applications
We can design and introduce synthetic open- or closed-loop genetic circuits into mammalian chassis to help our customers explore the potential of mammalian cells for medical applications.
- Disease diagnosis (e.g., detection of elevated uric acid levels, elevated fatty acid levels, and decreased blood pH levels).
- Drug discovery (e.g., screening for antibiotics and discovery of disease drivers).
- Development of therapeutics (e.g., gene therapy, CAR T-cell therapy, and protein-based therapies).
- Vaccine development (e.g., influenza vaccines and cancer vaccines).
- Disease treatment (e.g., treatment of obesity, diabetes, and cancer).
Want to Learn More?
CD Biosynsis has been continuously expanding our synthetic biology toolbox and keeping our knowledge and skills current. We provide full support for our customers' innovations in synthetic biology. If you require any further details, please feel free to contact us and let us know how we can support your new idea or project.
References
- Way J C, et al. Integrating biological redesign: where synthetic biology came from and where it needs to go. Cell, 2014, 157(1): 151-161.
- Lienert F, et al. Synthetic biology in mammalian cells: next generation research tools and therapeutics. Nature reviews Molecular cell biology, 2014, 15(2): 95-107.
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